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Interaction of a G-protein β-subunit with a conserved sequence in Ste20/PAK family protein kinases (1998)

Leeuw, Thomas ; Wu, Cunle ; Schrag, Joseph D. ; [et al.]

[s.l.] : Macmillan Magazines Ltd.

Nature 391 (1998), S. 191-195

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ISSN: 1476-4687

Source: Nature Archives 1869 - 2009

Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics

Notes: [Auszug] Serine/threonine protein kinases of the Ste20/PAK family have been implicated in the signalling from heterotrimeric G proteins to mitogen-activated protein (MAP) kinase cascades,. In the yeast Saccharomyces cerevisiae, Ste20 is involved in transmitting the mating-pheromone signal from the ...

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1038/34448

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Genetic interactions indicate a role for Mdg1p and the SH3 domain protein Bem1p in linking the G-protein mediated yeast pheromone signalling pathway to regulators of cell polarity (1996)

Leberer, E. ; Chenevert, J. ; Leeuw, Thomas ; [et al.]

Springer

Molecular genetics and genomics 252 (1996), S. 608-621

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ISSN: 1617-4623

Keywords: Key words Cell polarity ; G-protein ; MDG1 gene ; Signal transduction ; Yeast

Source: Springer Online Journal Archives 1860-2000

Topics: Biology

Notes: Abstract The pheromone signal in the yeast Saccharomyces cerevisiae is transmitted by the β and γ subunits of the mating response G-protein. The STE20 gene, encoding a protein kinase required for pheromone signal transduction, has recently been identified in a genetic screen for high-gene-dosage suppressors of a partly defective Gβ mutation. The same genetic screen identified BEM1, which encodes an SH3 domain protein required for polarized morphogenesis in response to pheromone, and a novel gene, designated MDG1 (multicopy suppressor of defective G-protein). The MDG1 gene was independently isolated in a search for multicopy suppressors of a bem1 mutation. The MDG1 gene encodes a predicted hydrophilic protein of 364 amino acids with a molecular weight of 41 kDa that has no homology with known proteins. A fusion of Mdg1p with the green fluorescent protein from Aequorea victoria localizes to the plasma membrane, suggesting that Mdg1p is an extrinsically bound membrane protein. Deletion of MDG1 causes sterility in cells in which the wild-type Gβ has been replaced by partly defective Gβ derivatives but does not cause any other obvious phenotypes. The mating defect of cells deleted for STE20 is partially suppressed by multiple copies of BEM1 and CDC42, which encodes a small GTP-binding protein that binds to Ste20p and is necessary for the development of cell polarity. Elevated levels of STE20 and BEM1 are capable of suppressing a temperature-sensitive mutation in CDC42. This complex network of genetic interactions points to a role for Bem1p and Mdg1p in G-protein mediated signal transduction and indicates a functional linkage between components of the pheromone signalling pathway and regulators of cell polarity during yeast mating.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/BF02172407

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Harmonised data from underway navigation, meteorology and surface water measurements during the Tara Oceans expedition in 2009-2013 (2017)

Boss, Emmanuel ; Picheral, Marc ; Searson, Sarah ; [et al.]

PANGAEA

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Publication Date: 2024-01-06

Description: The Tara Oceans Expedition (2009-2013) sampled the world oceans on board a 36 m long schooner, collecting environmental data and organisms from viruses to planktonic metazoans for later analyses using modern sequencing and state-of-the-art imaging technologies. Tara Oceans Data are particularly suited to study the genetic, morphological and functional diversity of plankton. During the entire expedition (2009-2013), underway measurements were obtained from a meteorological station (BATOS), a thermosalinograph (TSG, SBE 45), a Fast Repetition Rate Flurometer (FRRF, LIFT-FRR01), and a spectrophotometer (WETLabs AC-S). In 2013 underway measurements were enhanced by adding a Photosynthetically Available Radiation (PAR) sensor (QCP2350, Biospherical Instruments, Inc.), a laser spectrofluorometer (WETLabs ALFA, Chekalyuk et al., 2012) that replaced the FRRF, a spectral backscattering sensor (WETLabs Eco-bb3), a pCO2 sensor (ProOceanus CO2-Pro), a pH sensor (Satlantic, SeaFET) and a particle imaging system triggered by chlorophyll-a fluorescence (a prototype of McLane Research Laboratories' Imaging FlowCytobot, Sosik Lab, WHOI). Discrete measurements of CDOM absorption measurements were made using an Ultrapath (WPI Inc.) to calibrate the in-line AC-S. Hence the AC-S was also used to provide CDOM absorption in addition to particulate matter properties. The present collection includes three data sets that are harmonised with a common time stamp. The source data sets given in the reference section.

Keywords: Fondation Tara Expeditions; FondTara; Tara_Oceans_2009-2013; Tara Oceans Expedition

Type: Dataset

Format: application/zip, 3 datasets

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Underway surface water data during the Tara Oceans expedition in 2013 (2017)

Boss, Emmanuel ; Picheral, Marc ; Searson, Sarah ; [et al.]

PANGAEA

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Publication Date: 2024-02-17

Description: The Tara Oceans Expedition (2009-2013) sampled the world oceans on board a 36 m long schooner, collecting environmental data and organisms from viruses to planktonic metazoans for later analyses using modern sequencing and state-of-the-art imaging technologies. Tara Oceans Data are particularly suited to study the genetic, morphological and functional diversity of plankton. During the entire expedition (2009-2013), underway measurements were obtained from a meteorological station (BATOS), a thermosalinograph (TSG, SBE 45), a Fast Repetition Rate Flurometer (FRRF, LIFT-FRR01), and a spectrophotometer (WETLabs AC-S). In 2013 underway measurements were enhanced by adding a Photosynthetically Available Radiation (PAR) sensor (QCP2350, Biospherical Instruments, Inc.), a laser spectrofluorometer (WETLabs ALFA, Chekalyuk et al., 2012) that replaced the FRRF, a spectral backscattering sensor (WETLabs Eco-bb3), a pCO2 sensor (ProOceanus CO2-Pro), a pH sensor (Satlantic, SeaFET) and a particle imaging system triggered by chlorophyll-a fluorescence (a prototype of McLane Research Laboratories' Imaging FlowCytobot, Sosik Lab, WHOI). Discrete measurements of CDOM absorption measurements were made using an Ultrapath (WPI Inc.) to calibrate the in-line AC-S. Hence the AC-S was also used to provide CDOM absorption in addition to particulate matter properties. The present dataset contains surface water data measured during the 2013 campaigns of the Tara Oceans Expedition. Latitude and Longitude were obtained from TSG data. The time stamp of this data set is harmonised with that of other underway data in this collection.

Keywords: [CSSS]; [day/night] [surface water layer (ENVO:00002042)]; Aquatic Laser Fluorescence Analyzer (ALFA); Backscattering coefficient of particles, 470 nm; Backscattering coefficient of particles, 526 nm; Backscattering coefficient of particles, 660 nm; Basis of event; Campaign of event; Chlorophyll a; Comment of event; Continuous Surface Sampling System; DATE/TIME; DEPTH, water; Dudinka to Pevek, Stations: TARA_182-192; Event label; Fluorescence, chlorophyll; Fluorescence, colored dissolved organic matter; Fondation Tara Expeditions; FondTara; Fugacity of carbon dioxide (water) at equilibrator temperature (wet air); Ilulissat to Québec, Stations: TARA_208-210; LATITUDE; LONGITUDE; Lorient to Tromsø, Stations: TARA_155-166; Maximum photochemical quantum yield of photosystem II; Method/Device of event; Murmansk to Dudinka, Stations: TARA_167-181; NASA Ocean Biology & Biogeochemistry Programme; OBB; Optional event label; Particle size distribution slope; Particulate organic carbon; Pevek to Tuktoyaktuk, Stations: TARA_193-199; pH; pH sensor, Satlantic SeaFET; Québec to Lorient, Stations: none; registered at PANGAEA, Data Publisher for Earth and Environmental Science; registered at the European Nucleotides Archive (ENA); Salinity; Spectral backscattering sensor (WET Labs, Eco-bb3); Spectrophotometer, WET Labs, Inc., AC-S; Station label; SV Tara; TARA_20130519Z; TARA_20130519Z_UDW_EVENT_CSSS; TARA_20130629Z; TARA_20130629Z_UDW_EVENT_CSSS; TARA_20130801Z; TARA_20130801Z_UDW_EVENT_CSSS; TARA_20130907Z; TARA_20130907Z_UDW_EVENT_CSSS; TARA_20130921Z; TARA_20130921Z_UDW_EVENT_CSSS; TARA_20131020Z; TARA_20131020Z_UDW_EVENT_CSSS; TARA_20131116Z; TARA_20131116Z_UDW_EVENT_CSSS; Tara_Oceans_2009-2013; Tara Oceans Expedition; Temperature, water; Thermosalinograph; TSG; Tuktoyaktuk to Ilulissat, Stations: TARA_200-207; underway pCO2 measuring instrument (ProOceanus CO2-Pro)

Type: Dataset

Format: text/tab-separated-values, 345386 data points

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Underway surface water data during the Tara Oceans expedition in 2009-2012 (2017)

Boss, Emmanuel ; Picheral, Marc ; Searson, Sarah ; [et al.]

PANGAEA

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Publication Date: 2024-01-06

Description: The Tara Oceans Expedition (2009-2013) sampled the world oceans on board a 36 m long schooner, collecting environmental data and organisms from viruses to planktonic metazoans for later analyses using modern sequencing and state-of-the-art imaging technologies. Tara Oceans Data are particularly suited to study the genetic, morphological and functional diversity of plankton. During the entire expedition (2009-2013), underway measurements were obtained from a meteorological station (BATOS), a thermosalinograph (TSG, SBE 45), a Fast Repetition Rate Flurometer (FRRF, LIFT-FRR01), and a spectrophotometer (WETLabs AC-S). In 2013 underway measurements were enhanced by adding a Photosynthetically Available Radiation (PAR) sensor (QCP2350, Biospherical Instruments, Inc.), a laser spectrofluorometer (WETLabs ALFA, Chekalyuk et al., 2012) that replaced the FRRF, a spectral backscattering sensor (WETLabs Eco-bb3), a pCO2 sensor (ProOceanus CO2-Pro), a pH sensor (Satlantic, SeaFET) and a particle imaging system triggered by chlorophyll-a fluorescence (a prototype of McLane Research Laboratories' Imaging FlowCytobot, Sosik Lab, WHOI). Discrete measurements of CDOM absorption measurements were made using an Ultrapath (WPI Inc.) to calibrate the in-line AC-S. Hence the AC-S was also used to provide CDOM absorption in addition to particulate matter properties. The present dataset contains surface water data measured during the 2009-2012 campaigns of the Tara Oceans Expedition. Latitude and Longitude were obtained from TSG data. The time stamp of this data set is harmonised with that of other underway data in this collection.

Keywords: [CSSS]; [day/night] [surface water layer (ENVO:00002042)]; Abu Dhabi to Muscat, Stations: none; Algiers to Barcelona, Stations: TARA_008-011; ALTITUDE; Ascencion to Rio de Janiero, Stations: TARA_072-076; Athens to Beyrouth, Stations: TARA_027-028; Atseranana to Mamoudzou, Stations: TARA_053-054; Barcelona to Nice, Stations: none; Basis of event; Bermuda to Horta, Stations: TARA_148-151; Beyrouth to Alexandria, Stations: TARA_029-030; Bizerte to Naples, Stations: TARA_014; Buenos Aires to Ushuaia, Stations: TARA_079b-083; Campaign of event; CapeTown to Ascencion, Stations: TARA_067-071; Chlorophyll a; Comment of event; Continuous Surface Sampling System; DATE/TIME; Dubrovnik to Athens, Stations: TARA_025-026; Easter Island to Guayaquil, Stations: TARA_098-102; Event label; Fast repetition rate fluorometry (FRRF) (Kolber & Falkowski, 1993); Fluorescence, maximum; Fluorescence, minimum; Fondation Tara Expeditions; FondTara; Functional absorption cross section; Guayaquil to Puerto Ayora, Stations: TARA_103-106; Guayaquil to Totegegie, Stations: TARA_110-113; Honolulu to San Diego, Stations: TARA_131-135; Horta to La Coruna, Stations: TARA_152-154; Initial slope of the photosynthesis/irradiance relationship; Irradiance half-saturation level; Jeddah to Djibouti, Stations: TARA_034; LATITUDE; Lisbon to Tangier, Stations: TARA_003-004; LONGITUDE; Lorient to Lisbon, Stations: TARA_001-002; Malé to Saint Brandon, Stations: TARA_044-049; Mamoudzou to Cape Town, Stations: TARA_055-066; Maximum level of photosynthetic electron transport; Maximum photochemical quantum yield of photosystem II; Method/Device of event; Mumbai to Malé, Stations: TARA_041-043; Muscat to Mumbai, Stations: TARA_036-040; Naples to Valetta, Stations: TARA_015-017; NASA Ocean Biology & Biogeochemistry Programme; New-York City to Bermuda, Stations: TARA_146-147; Nice to Bizerte, Stations: TARA_012-013; OBB; Optional event label; Panama City to Savannah, Stations: TARA_141-143; Papeete to Honolulu, Stations: TARA_126-130; Particle size distribution slope; Particulate organic carbon; Plastoquinone pool size; Port Louis to Saint Denis, Stations: TARA_050-051; Puerto Ayora to Guayaquil, Stations: TARA_107-109; Puerto Montt to Valparaiso, Stations: TARA_090-092; Puerto Williams to Puerto Montt, Stations: none; registered at PANGAEA, Data Publisher for Earth and Environmental Science; registered at the European Nucleotides Archive (ENA); Rio de Janiero to Buenos Aires, Stations: TARA_077-079a; Saint Brandon to Saint Brandon, Stations: Coral sampling; Saint Denis to Antseranana, Stations: TARA_052; Salinity; San Diego to Panama City, Stations: TARA_136-140; Savannah to New-York City, Stations: TARA_144-145; Sharm El Sheikh to Jeddah, Stations: TARA_031-033; Spectrophotometer, WET Labs, Inc., AC-S; Station label; SV Tara; Taiohae harbour to Papeete, Stations: TARA_122-125; Tangier to Algiers, Stations: TARA_005-007; TARA_20090905Z; TARA_20090905Z_UDW_EVENT_CSSS; TARA_20090913Z; TARA_20090913Z_UDW_EVENT_CSSS; TARA_20090919Z; TARA_20090919Z_UDW_EVENT_CSSS; TARA_20090926Z; TARA_20090926Z_UDW_EVENT_CSSS; TARA_20091004Z; TARA_20091004Z_UDW_EVENT_CSSS; TARA_20091011Z; TARA_20091011Z_UDW_EVENT_CSSS; TARA_20091019Z; TARA_20091019Z_UDW_EVENT_CSSS; TARA_20091025Z; TARA_20091025Z_UDW_EVENT_CSSS; TARA_20091031Z; TARA_20091031Z_UDW_EVENT_CSSS; TARA_20091111Z; TARA_20091111Z_UDW_EVENT_CSSS; TARA_20091122Z; TARA_20091122Z_UDW_EVENT_CSSS; TARA_20091128Z; TARA_20091128Z_UDW_EVENT_CSSS; TARA_20091214Z; TARA_20091214Z_UDW_EVENT_CSSS; TARA_20100108Z; TARA_20100108Z_UDW_EVENT_CSSS; TARA_20100118Z; TARA_20100118Z_UDW_EVENT_CSSS; TARA_20100302Z; TARA_20100302Z_UDW_EVENT_CSSS; TARA_20100309Z; TARA_20100309Z_UDW_EVENT_CSSS; TARA_20100327Z; TARA_20100327Z_UDW_EVENT_CSSS; TARA_20100410Z; TARA_20100410Z_UDW_EVENT_CSSS; TARA_20100423Z; TARA_20100423Z_UDW_EVENT_CSSS; TARA_20100508Z; TARA_20100508Z_UDW_EVENT_CSSS; TARA_20100515Z; TARA_20100515Z_UDW_EVENT_CSSS; TARA_20100523Z; TARA_20100523Z_UDW_EVENT_CSSS; TARA_20100624Z; TARA_20100624Z_UDW_EVENT_CSSS; TARA_20100905Z; TARA_20100905Z_UDW_EVENT_CSSS; TARA_20101003Z; TARA_20101003Z_UDW_EVENT_CSSS; TARA_20101031Z; TARA_20101031Z_UDW_EVENT_CSSS; TARA_20101126Z; TARA_20101126Z_UDW_EVENT_CSSS; TARA_20101231Z; TARA_20101231Z_UDW_EVENT_CSSS; TARA_20110203Z; TARA_20110203Z_UDW_EVENT_CSSS; TARA_20110220Z; TARA_20110220Z_UDW_EVENT_CSSS; TARA_20110311Z; TARA_20110311Z_UDW_EVENT_CSSS; TARA_20110401Z; TARA_20110401Z_UDW_EVENT_CSSS; TARA_20110501Z; TARA_20110501Z_UDW_EVENT_CSSS; TARA_20110509Z; TARA_20110509Z_UDW_EVENT_CSSS; TARA_20110520Z; TARA_20110520Z_UDW_EVENT_CSSS; TARA_20110626Z; TARA_20110626Z_UDW_EVENT_CSSS; TARA_20110716Z; TARA_20110716Z_UDW_EVENT_CSSS; TARA_20110726Z; TARA_20110726Z_UDW_EVENT_CSSS; TARA_20110826Z; TARA_20110826Z_UDW_EVENT_CSSS; TARA_20110928Z; TARA_20110928Z_UDW_EVENT_CSSS; TARA_20111124Z; TARA_20111124Z_UDW_EVENT_CSSS; TARA_20111230Z; TARA_20111230Z_UDW_EVENT_CSSS; TARA_20120126Z; TARA_20120126Z_UDW_EVENT_CSSS; TARA_20120212Z; TARA_20120212Z_UDW_EVENT_CSSS; TARA_20120223Z; TARA_20120223Z_UDW_EVENT_CSSS; TARA_20120314Z; TARA_20120314Z_UDW_EVENT_CSSS; Tara_Oceans_2009-2013; Tara Oceans Expedition; Temperature, water; Thermosalinograph; Time constant, electron transport from plastoquinone to photosystem I pool; Time constant, electron transport from primary electron acceptor to plastoquinone pool; Totegegie to Taiohae harbour, Stations: Coral sampling; Totegegie to Totegegie, Stations: TARA_114-121; TSG; Ushuaia to Puerto Williams, Stations: TARA_084-089; Valetta to Dubrovnik, Stations: TARA_020-024; Valetta to Tripoli, Stations: TARA_018; Valparaiso to Easter Island, Stations: TARA_093-097

Type: Dataset

Format: text/tab-separated-values, 1249146 data points

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[RAW VALIDATED DATA] Properties of seawater and particulate matter from a WETLabs AC-S spectrophotometer and a WETLabs chlorophyll fluorometer mounted on the continuous surface water sampling system during the Tara Oceans expedition 2009-2013 (2014)

Boss, Emmanuel ; Picheral, Marc ; Slade, Wayne ; [et al.]

PANGAEA

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Publication Date: 2024-01-06

Description: The Tara Oceans Expedition (2009-2013) was a global survey of ocean ecosystems aboard the Sailing Vessel Tara. It carried out extensive measurements of evironmental conditions and collected plankton (viruses, bacteria, protists and metazoans) for later analysis using modern sequencing and state-of-the-art imaging technologies. Tara Oceans Data are particularly suited to study the genetic, morphological and functional diversity of plankton. The present data publication provides raw validated data files containing measurements from the Continuous Surface Sampling System [CSSS]. Water was pumped at the front of the vessel from ~2m depth, then de-bubbled and circulated to a WETLabs AC-S spectrophotometer and a WETLabs chlorophyll fluorometer. Systems maintenance (instrument cleaning, flushing) was done approximately once a week and in port between successive legs. All data were stamped with a GPS.

Keywords: Fondation Tara Expeditions; FondTara; NASA Ocean Biology & Biogeochemistry Programme; OBB; Tara_Oceans_2009-2013; Tara Oceans Expedition

Type: Dataset

Format: application/zip, 154.5 MBytes

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