ISSN:
1013-9826
Source:
Scientific.Net: Materials Science & Technology / Trans Tech Publications Archiv 1984-2008
Topics:
Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
Notes:
This study was designed to investigate the influence of demineralized bone particles(DBP)/PLGA hybrid scaffold on angiogenesis and osteogenesis in a calvarial defect model.DBP/PLGA scaffolds were manufactured by solvent casting/salt leaching method, and each scaffoldcontained 0, 10, 20, 40, and 80 wt% DBP of PLGA, respectively. A total of 34 rats were operatedand bicortical holes were placed on their calvaria. The defects were filled with different ratioDBP/PLGA scaffolds. After 3, 7, 14, and 28 days, specimens were taken and, histologic,immunohistologic and RT-PCR analyses were carried out concerning number of vessels and densityof regenerated bone, and angiogenic activation. On days 7, in all experimental groups, boneformation occurred in a direction from defected margin of calvarium to center of implanted scaffoldand new vessel formation took place in front of the osteogenic regeneration front. We found that the20 and 40 wt% DBP/PLGA scaffold was superior in its ability to regenerate new bone, inducedmore intensive formation of microvasculature and expressed in a higher level of osteocalcin mRNAthan other groups
Type of Medium:
Electronic Resource
URL:
http://www.tib-hannover.de/fulltexts/2011/0528/01/54/transtech_doi~10.4028%252Fwww.scientific.net%252FKEM.342-343.161.pdf
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