Publication Date:
2024-03-06
Description:
The present dataset contains the source data for Figure 2B of Tentner et al. (2012). The data shows the percentage of cultured cell-populations that stained positively and/or negatively for apoptotic markers cleaved caspase-3 and cleaved PARP, following DNA damage treatments induced by various doses of doxorubicin (0, 2 and 10 µmole/L) in the presence (100 ng/mL) or absence (0 ng/mL) of TNF-alpha co-treatment. For the six treatment conditions investigated, cell counts were made by flow cytometry at times 6, 12, 24, and 48 h following treatment; CULTURE DETAILS: U2OS cells were obtained from ATCC were maintained at 21% oxygen and 5% CO2 in Dulbecco's modified Eagle medium supplemented with 10% fetal bovine serum, penicillin, streptomycin, 2mM L-glutamine, and used within 15–20 passages. The first thymidine block was released by washing the plates three times with PBS, and incubating them in fresh thymidine-free media for 12 h. A second thymidine block was then performed by re-addition of thymidine to 2.5 mM followed by incubation for an additional 18 h. Media was aspirated, plates were washed 3 with PBS, and replaced with fresh media in the presence or absence of 10 mM aphidicolin; ANALYSIS DETAILS: See supplementary journal publication; RESULT: The authors of the supplementary journal publication conclude that TNF enhances dose-dependent cell death following doxorubicin-induced DNA damage with minimal affect on dose-dependent cell-cycle arrest.
Keywords:
775607_Experiment_Culture_Treatment_a; 775607_Experiment_Culture_Treatment_b; 775607_Experiment_Culture_Treatment_c; 775607_Experiment_Culture_Treatment_d; 775607_Experiment_Culture_Treatment_e; 775607_Experiment_Culture_Treatment_f; Cell counts, percent of total; Event label; Flow cytometry; Marker: apoptosis, cleaved caspase-3; Marker: apoptosis, cleaved PARP; Treatment: chemical concentration, of doxorubicin; Treatment: chemical concentration, of TNF alpha; Treatment: time after
Type:
Dataset
Format:
text/tab-separated-values, 3744 data points
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