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  • 1
    Electronic Resource
    Electronic Resource
    Growth and nitrate uptake properties of plants grown at different relative rates of nitrogen supply. I. Growth of Pisum and Lemna in relation to nitrogen (1989)
    Oxford, UK : Blackwell Publishing Ltd
    Plant, cell & environment 12 (1989), S. 0 
    Details
    ISSN: 1365-3040
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Abstract The relations between growth and internal nitrogen concentrations were investigated in nonnodulated Pisum sativum L. cv. Marma and Lemna gibba L. grown at relative rates of nitrate-N additions (RA) varying from 0.03 to 0.27 d 1(Pisum) and 0.05 to 0.40 d 1 (Lemna). At RA≤0.21 d 1(Pisum) and ≤0.30 d 1 (Lemna), the relative growth rate (RGR) correlated well with RA whereas higher RA was not met by any further increawse in growth rate. The tissue nitrogen concentrations at growth-limiting RA increased linearly with RGR. The slope of these lines indicate a maximum nitrogen productivity (amount of biomass formed per unit nitrogen and time) of 14.4 g DW g 1 Nd 1 for Pisum and 15.9 g DW g 1 N d 1 for Lemna. Extrapolation of the plots to RGR=0 yielded intercepts of 10–15 mg N g−1 DW for Pisum tissue, whereas for Lemna the intercepts were closer to the origin than for Pisum. These intercepts formally define a fraction of the total plant nitrogen that appears not to be active in production of new biomass, her termed ‘non-growth nitrogen’. The partitioning of nitrogen as well as biomass to the roots increased at low RA, and is discussed in relation to activity of shoots and roots, respectively.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-3040.1989.tb01639.x
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  • 2
    Electronic Resource
    Electronic Resource
    Preparation of algal extracts for bacterial luciferase assay of pyridine nucleotides
    Amsterdam : Elsevier
    Plant Science 45 (1986), S. 189-194 
    Details
    ISSN: 0168-9452
    Keywords: bacterial luciferase ; cyanobacteria ; extraction procedures ; green algae ; nicotineamide adeninedinucleotide (phosphate)
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology
    Type of Medium: Electronic Resource
    URL: http://linkinghub.elsevier.com/retrieve/pii/0168-9452(86)90138-X
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  • 3
    Electronic Resource
    Electronic Resource
    Nitrate regulation of zeation riboside levels in barley roots: effects of inhibitors of N assimilation and comparison with ammonium
    Amsterdam : Elsevier
    Plant Science 93 (1993), S. 77-84 
    Details
    ISSN: 0168-9452
    Keywords: Cytokinins ; Nitrate ; Nitrogen assimilation ; Zeatin riboside
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology
    Type of Medium: Electronic Resource
    URL: http://linkinghub.elsevier.com/retrieve/pii/0168-9452(93)90036-Y
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  • 4
    Electronic Resource
    Electronic Resource
    Growth and nitrate uptake properties of plants grown at different relative rates of nitrogen supply. II. Activity and affinity of the nitrate uptake system in Pisum and Lemna in relation to nitrogen availability and nitrogen demand (1989)
    Oxford, UK : Blackwell Publishing Ltd
    Plant, cell & environment 12 (1989), S. 0 
    Details
    ISSN: 1365-3040
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Abstract Net nitrate uptake rates were measured and the kinetics calculated in non-nodulated Pisum sativum L. cv. Marma and Lemna gibba L. adapted to constant relative rates of nitrate-N additions (RA), ranging from 0.03 to 0.27 d−1 for Pisum and from 0.05 to 0.40 d−1 for Lemna, Vmax of net nitrate uptake (measured in the range 10 to 100 mmol m−3 nitrate, i.e. ‘system I’) increased with RA in the growth limiting range but decreased when RA exceeded the relative growth rate (RGR), Km was not significantly related to changes in RA. On the basis of previous 13N-flux experiments, it is concluded that the differences in Vmax at growth limiting RA are attributable to differences in influx rates. Linear relationships between Vmax and tissue nitrogen concentrations were obtained in the growth limiting range for both species, and extrapolated intercepts relate well with the previously defined minimal nitrogen concentrations for plant growth (Oscarson, Ingemarsson & Larsson, 1989). Analysis of Vmax for net nitrate uptake on intact plant basis in relation to nitrogen demand during stable, nitrogen limited, growth shows an increased overcapacity at lower RA values in both species, which is largely explained by the increased relative root size at low RA. A balancing nitrate concentration, defined as the steady state concentration needed to sustain the relative rate of increase in plant nitrogen (RN), predicted by RA, was calculated for both species. In the growth limiting range, this value ranges from 3.5 mmol m−3 (RA 0.03 d−1) to 44 mmol m−3 (RA 0.21 d−1) for Pisum and from 0.2 mmol m−3 (RA 0.05 d−1) to 5.4 mmol m−3 (RA 0.03 d−1) for Lemna. It is suggested that this value can be used as a unifying measure of the affinity for nitrate, integrating the performance of the nitrate uptake system with nitrate flux and long term growth and demand for nitrogen.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-3040.1989.tb01640.x
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  • 5
    Electronic Resource
    Electronic Resource
    Translocation and cycling through roots of recently absorbed nitrogen and sulphur in wheat (Triticum aestivum) during vegetative and generative growth (1991)
    Oxford, UK : Blackwell Publishing Ltd
    Physiologia plantarum 82 (1991), S. 0 
    Details
    ISSN: 1399-3054
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: 15N-Nitrate and 35S-sulphate labelling experiments were performed with spring wheat (Triticum aestivum L. cv. Timmo) 44. 64, 79, 95 and 115 days after sowing (growth stages arbitrarily denoted I to V). Label was fed to the plants via a fraction of the root system, termed “donor root”, whereas the rest of the root (“receiver root”) was fed non-labelled nutrient solution. Net uptake rates for both nitrate and sulphate per unit root weight changed little from growth stage I to IV, but were considerably lower at stage V. On a whole-plant weight basis, uptake declined from stage I to IV, because root contribution to total plant weight declined. Between 80 and 95% of absorbed label was translocated to the shoot at all growth stages. At stage V, up to 30% of absorbed label was recovered in the ears. Labelling of the receiver root indicated that, at all growth stages, 10 to 17% of N and 12 to 32% of S translocated to the shoot was retranslocated to the root. This corresponds to between 35 and 85% of the label actually recovered in the roots. Analysis of 15N-labelling of xylem sap collected from receiver roots at growth stages I to IV indicated that about half of the reduced N in the sap is derived from cycling through roots of recently assimilated N. Evidence of cycling was also obtained at stage V. Labelled sulphate was the only form of S cycled in the plant, but it accounted for only 1 to 7% of the sulphate in the xylem sap.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1399-3054.1991.tb02916.x
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  • 6
    Electronic Resource
    Electronic Resource
    Relations between uptake and utilization of NO−3 in Pisum growing exponentially under nitrogen limitation (1986)
    Oxford, UK : Blackwell Publishing Ltd
    Physiologia plantarum 67 (1986), S. 0 
    Details
    ISSN: 1399-3054
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: The utilization and translocation of nitrogen was investigated in exponentially growing, nitrogen-limited Pisum sativum L. cv. Marma. The plants were given N daily at exponentially increasing, although suboptimal, relative nitrogen addition rates (RN) calculated to yield a relative increment in N of 0.06 day−1 and 0.12 day−1. After 10 days of NO−3 additions (26 days after sowing), the relative growth rate more or less equaled RN. Uptake of NO−3 was several-fold higher than the N requirement for the growth rate set by RN. The daily addition of NO−3 was taken up after 7 to 8 h, resulting in a cyclic behaviour in the NO−3 utilization. During the phase of net NO−3 influx, the filling phase (0 to 8 h), in vitro nitrate reductase activity (NR activity) and intracellular levels of soluble N in the root increased. In the phase of no net influx of NO−3 the depletion phase (8 to 24 h), the plants were entirely dependent on stored N. During this phase both in vitro NR activity and intracellular levels of soluble N decreased. Also the calculated actual rate of NO−3 reduction was high in the filling phase, while it was close to zero in the depletion phase. The pattern of these fluctuations indicates that the regulation of NO−3 utilization involves an interplay between transmembrane fluxes of NO−3, the cytosolic NO−3 concentration and NR activity. Cyclic fluctuations in N-containing compounds were also found in the xylem. Nitrogen was mainly transported as amino acids. The pattern of NO−3 transport in the xylem and the fluctuations in the shoot of in vitro NR activity indicate that a reasoning similar to that for the regulation of NO−3 assimilation in the root also applies for the shoot. The results also indicate a substantial supply of amino acids to the xylem through recirculation from the shoot.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1399-3054.1986.tb01271.x
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  • 7
    Electronic Resource
    Electronic Resource
    Photophosphorylation in Scenedesmus in vivo: O2 evolution, ATP pools and transients, and phosphate binding during photoreduction of NO3−, NO2− and CO2 (1980)
    Oxford, UK : Blackwell Publishing Ltd
    Physiologia plantarum 48 (1980), S. 0 
    Details
    ISSN: 1399-3054
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: The relation between light-induced electron transport with NO3−, NO2− or CO2 as acceptors, ATP pools and transients in dark-light-dark transitions, and phosphate uptake was examined in phosphorus-starved cells of Scenedesmus obtusiusculus Chod. Net O2 evolution at saturating light was around 6 μmol × (mg chlorophyll × h)−1 in the absence of any acceptor, but reached average rates of 21, 65 and 145 μmol × (mg chlorophyll × h)−1 upon additions of 5 mM KNO3, KNO2 and KHCO3, respectively. The apparent rate of photophosphorylation in transition experiments was only a few percent of the rate calculated from CO2-dependent O2 evolution. Blocking non-cyclic electron transport with DCMU inhibited phosphate assimilation, but acceleration of non-cyclic electron flow by addition of NO3− or NO2− did not stimulate phosphate assimilation as compared to the situation without an acceptor. A functional non-cyclic system might primarily be needed for an efficient shuttle transfer of ATP from the chloroplast to the cytoplasm. An inhibition of the non-cyclic system due to lack of reducible substrates accelerates the cyclic system and thus indicates a regulation mechanism between the two systems.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1399-3054.1980.tb03263.x
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  • 8
    Electronic Resource
    Electronic Resource
    Light-Induced Phosphate Binding in Relation to Photophosphorylation and Levels of ATP, ADP and AMP in the Green Alga Scenedesmus (1978)
    Oxford, UK : Blackwell Publishing Ltd
    Physiologia plantarum 44 (1978), S. 0 
    Details
    ISSN: 1399-3054
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Synchronous cultures of Scenedesmus obtusiusculus Chod. were starved for phosphorus for 48 h. Such cells develop an efficient mechanism for phosphate binding which is very sensitive to metabolic inhibitions. Phosphate binding, fluctuations in the ATP pool during dark-light-dark transitions, and steady state levels of ATP, ADP and AMP were studied. The experiments were carried out in a CO2-free N2 atmosphere. DCMU, phloridzin and 2,5-dibromo-3-methyl-6-isopropyl-p-benzoquinone (DBMIB) were used as inhibitors of photophosphorylation.Light-induced phosphate uptake was inhibited to various extents by all the inhibitions. The dark-light-dark transition experiments show that neither the light-induced increment in ATP nor the decrease at darkening are affected by DCMU, but DBMIB and phloridzin inhibit both processes. DCMU seems to affect the regulation of the ATP pool size. The steady state levels of the adenylate pools were almost the same in the light as in the dark, and they were also little sensitive to the inhibitors. In unpoisoned cells in the light the steady state ATP/ADP ratio was 1.7 and the energy charge was 0.66.The rates of phosphate binding are not correlated to any of the adenylate parameters studied. This is probably due to the diverse effects of the inhibitors on light-stimulated production of reducing equivalents, photophosphorylation and transfer of energy from the chloroplast to the cytoplasm.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1399-3054.1978.tb01624.x
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  • 9
    Electronic Resource
    Electronic Resource
    Short-term studies of NO 3 − uptake in Pisum using 13NO 3 − (1987)
    Springer
    Planta 170 (1987), S. 550-555 
    Details
    ISSN: 1432-2048
    Keywords: Nitrate (uptake, influx, efflux) ; Nitrogen (limination) ; Pisum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Influx, efflux and net uptake of NO 3 − was studied in Pisum sativum L. cv. Marma in short-term experiments where 13NO 3 − was used to trace influx. The influx rate in N-limited plants was similar both during net uptake at external concentrations of around 50 μM, and at low external NO 3 − concentrations (4–6 μM) when net uptake was practically zero. Efflux could be inferred from discrepancies between influx and net uptake but was never very high in the N-limited plants during net uptake. Close to the threshold concentration for not NO 3 − uptake, efflux was high and equalled influx. Thus, the threshold concentration can be regarded as a NO 3 − compensation point. The inclusion of NH 4 + in the outer medium decreased influx by about 40% but did not significantly affect efflux. The roles of NO 3 − fluxes and nitrate-reductase activity in regulating/limiting NO 3 − utilization are discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00402990
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  • 10
    Electronic Resource
    Electronic Resource
    Characteristics of NO 3 − uptake in lemna and pisum (1988)
    Springer
    Plant and soil 111 (1988), S. 203-205 
    Details
    ISSN: 1573-5036
    Keywords: efflux ; influx ; kinetics ; net uptake ; nitrate
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02139939
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