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  • 1
    Electronic Resource
    Electronic Resource
    [s.l.] : Nature Publishing Group
    Nature 177 (1956), S. 336-337 
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] Various workers have shown that the iron determined by leaf analysis is considerably less after washing the leaves either in water containing a detergent or in dilute hydrochloric acid3. This difference has been assumed to result from the removal of iron contamination on leaf surfaces, although it ...
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  • 2
    Electronic Resource
    Electronic Resource
    [s.l.] : Nature Publishing Group
    Nature 189 (1961), S. 312-312 
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] Tomato plants were raised from seed germinated.in acid-washed sand and grown in an iron-free nutrient solution in the glasshouse. Ten days after the first true leaves had appeared, three plants of uniform size were transferred to each of six culture dishes. The nutrient solution included 1 p.p.m. ...
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  • 3
    ISSN: 1399-3054
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Field bean (Vicia faba L.) cv. Maris Bead seeds were inoculated with Rhizobium Catalogue No. 1001, supplied by Rothamsted Experimental Station, and grown in sand culture supplied with a complete nutrient solution which included nitrate at either 1.5 or 6.0 mM. Nodules were detached from the roots at intervals during plant development and their rates of nitrogen fixation estimated by both acetylene reduction and 15N gas technique.There was a constant relationship, independent of nitrate supply, between the results obtained by these two methods at all samplings. The amounts of acetylene reduced divided by a factor of 5.75 gave the amount of true nitrogen fixation; this factor is about twice the theoretical value. It is suggested that this discrepancy arose because, with acetylene, all the electrons available to the nitrogenase were used to form ethylene, whereas during normal fixation only about half the electron supply was used to fix nitrogen, the remainder having been consumed in the production of hydrogen gas.
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  • 4
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Physiologia plantarum 48 (1980), S. 0 
    ISSN: 1399-3054
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Field bean (Vicia faba L.) cv. Maris Bead seeds were inoculated with Rhizobium Catalogue No. 1001, supplied by Rothamsted Experimental Station and grown in sand culture supplied with 15N-labelled nitrate at two concentrations. Plants were sampled at intervals throughout their growth for 15N and total N analysis. The rate of nitrate uptake was almost uniform up to pod-fill and was proportional to the nitrate concentration. Nodule weight was slightly depressed by the larger nitrate concentration at all samplings, and there was a corresponding reduction in the amount of atmospheric nitrogen fixed. However, at harvest the bean seeds from plants given most nitrate contained slightly more total N, as the enhanced nitrate uptake outweighed the reduction in fixation.
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  • 5
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Physiologia plantarum 60 (1984), S. 0 
    ISSN: 1399-3054
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Whole bean plants, ev. Cockfield, grown in pots crowded or well-spaced (50 or 10 plants m2, respectively) were treated with 14CO2 at the pod-fill stage (25 modes) and the radioactivity in each leaf was determined after 30 min. With spaced plants the uptake was greatest in the mid-stem leaves and was proportional to leaf area. In contrast, 70% of the total assimilation took place in the upper six leaves of crowded plants and there was a steady decrease down the stem.When 14CO2 was fed to single leaves of similar crowded plants the resultant distribution of labelled assimilates varied with the position of the treated leaf. After 6 h, 67% of the 14C fixed by a mid-stem leaf (node 13) was recovered from the beans, whereas 76% of that from an upper leaf (node 23) had accumulated along the stem. Due to the shading of mid-stem leaves at the higher planting densities, seed yield becomes increasingly dependent upon re-distribution of assimilates from stem to beans.
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  • 6
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Physiologia plantarum 51 (1981), S. 0 
    ISSN: 1399-3054
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: When whole plants were exposed to 14CO2, almost the same amount of radioactivity was taken up initially by each leaf regardless of its position on the stem and of the presence of beans at that node. Thus, although developing beans are a powerful sink for assimilated carbon, they do not increase the CO2 uptake by adjoining leaves.The distribution of labelled assimilates 6 hours after feeding 14CO2 to a single leaf for 1 hour varied with both the position of the treated leaf and the stage of development of the plant. Before any flowers were set most of the radioactivity from all expanded leaves moved downwards to the roots and the stem below the treated leaf (lower stem). Later, during pod-fill, the upper leaves maintained this supply to the roots and lower stem, whilst most of the carbon translocated from the lower and mid-stem leaves went to the beans. However, we found no exclusive relationship between a leaf and the supply to beans developing on the same node.The amount of radioactivity moving out of a source leaf at a fruiting node increased over successive samplings up to 48 h; the pattern of distribution of the 14CO2 however remained virtually unchanged.
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  • 7
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Physiologia plantarum 29 (1973), S. 0 
    ISSN: 1399-3054
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: At intervals through the year a study was made of the absorption and metabolism of arginine-C 14(U) which had been perfused into the wood of apple stem internodes. Absorption was greater during the period May to September than at any other time of the year. The metabolic breakdown of arginine took place only after absorption but the products found varied widely with season. From the analyses it is concluded that arginine is metabolized by the Krebs-Henseleit cycle at all times of the year, in addition to which during the period from May to August there are other pathways in operation that result in a more extensive breakdown so that all the N in arginine becomes available for new growth.
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  • 8
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Physiologia plantarum 38 (1976), S. 0 
    ISSN: 1399-3054
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: The absorption of nitrate by field bean plants at different times of development was investigated to determine the distribution and subsequent utilization of N up to the maturity of the beans.Nodulated plants were grown in sand culture and pulse-labelled with 15NO3 for 4 short periods during development. Whole plants were sampled at intervals until maturity and analysed for total nitrogen and 15N.There was a rapid increase in the bean dry weight and total N after fruit set and, at maturity, the beans contained about three times as much N as had ever been in the rest of the plant. Nitrate absorption continued steadily throughout plant development but nodule N fixation made a progressively greater contribution to the total N in the plant. Over a quarter of the total 15N absorbed was found in the roots immediately after each treatment and little of this was subsequently translocated upwards in spite of the large increase in shoot N taking place. Above ground the most powerful “sink” for recently absorbed and redistributed N was initially the youngest vegetative tissue. However after fruit set competition developed between the growing tip and the developing beans until at the later stages the beans became the more powerful. Quantitatively redistribution made only a small contribution to bean N.
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  • 9
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Physiologia plantarum 29 (1973), S. 0 
    ISSN: 1399-3054
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: A study was made of the absorption and metabolism of arginine-guanido-14C, citrulline-carbamyl-14C and ornithine 1-14C which had been applied to apple stem internodes by a perfusion technique at intervals throughout the year.The results showed that these amino acids were interconverted according to the Krebs-Henseleit cycle always in the direction arginine-ornithine-citrulline-arginine. In addition ornithine was degraded by another pathway with loss of CO2. This loss was particularly extensive during the period June to August when labelled proline and glutamic acid were also found. The evidence is consistent with the initial breakdown of ornithine taking place via glutamic semi-aldehyde.
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  • 10
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Physiologia plantarum 28 (1973), S. 0 
    ISSN: 1399-3054
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Solutions of amino acids are introduced rapidly into the xylem of short lengths of stem. At sampling the intact stem is first perfused with a salt solution to remove the amino acids still reversibly adsorbed by cation exchange. This fraction of the applied amino acids is thus distinguished from that absorbed into the tissues which is subsequently extracted from the disintegrated stem by aqueous ethanol.The potential value of this technique is discussed and illustrated with some results using carbon-14 labelled amino acids in apple stems.
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