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  • 1
    Electronic Resource
    Electronic Resource
    Lipid and protein histochemistry of enamel — Effects of fluoride (1977)
    Springer
    Calcified tissue international 24 (1977), S. 119-125 
    Details
    ISSN: 1432-0827
    Keywords: Protein ; Lipid ; Histochemistry ; Enamel
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Physics
    Notes: Summary Staining reactions for a number of histochemical procedures for lipophilic staining and protein were studied in the enamel matrix along the length of rat incisors. Sudan Black gave a positive stain across the whole thickness of very early enamel (up to 30 μm) but this staining only continued as a narrow band close to the ameloblasts as the enamel matured. A variety of tests for protein produced almost identical staining patterns in enamel matrix up to 100 μm thick. Since the pattern of “lipid” staining persisted, after using a number of procedures which could normally be expected to remove lipid, it is suggested that Sudan Black positive staining may be due to lipophilic protein rather than lipid itself. Fluoride did not significantly alter the staining reactions for “lipid” and protein but did proceduce matrix which was much more effectively stained by cross-linking agents FFDNB and FF sulphene.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02223305
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  • 2
    Electronic Resource
    Electronic Resource
    A microspectrophotometric analysis of the effect of fluoride on immature enamel matrix protein of rat molar teeth (1980)
    Springer
    Calcified tissue international 30 (1980), S. 57-66 
    Details
    ISSN: 1432-0827
    Keywords: Dental enamel ; Sodium fluoride ; Microspectrophotometry ; Enamel matrix protein ; Amelogenesis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Physics
    Notes: Summary Over a 24-h period, Wistar rats from 4 litters, 6 to 9 days old, were given five intraperitoneal injections of a solution of 0.9% sodium chloride containing sodium fluoride (3 mg F/kg body weight). Within-litter controls were used. All rats were killed by decapitation 2 h after the final injection and the rat heads, cut sagittally, were processed for protein histochemistry. The intensity of staining of the protein in the enamel matrix of the upper jaw molar tooth buds was quantified using the two-wavelength method of microphotometry. A significant increase in the intensity of staining of fluoride-treated tissues over controls was observed with the histochemical methods specific for arginine (P〈0.01), tyrosine (P 〈 0.05), and cysteine (P〈0.05). Other histochemical methods specific for amino acid groups failed to show any significant difference between fluoride and non-fluoride-treated enamel matrix.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02408607
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  • 3
    Electronic Resource
    Electronic Resource
    Calcitonin cell population and distribution in the thyroid gland of the rat (1985)
    New York, NY : Wiley-Blackwell
    Journal of Morphology 186 (1985), S. 271-278 
    Details
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Calcitonin-containing cells (C cells) were identified in male Wistar white rats using an immunoperoxidase technique. They occupied a central position within the thyroid; very few were found peripherally, inferiorly, and superiorly; and none were present in the isthmus. The number of calcitonin-containing cells present per gram of body weight increased with age up to 70 days and had declined by 100 days. Determining the true total C-cell count through the entire thyroid is a very laborious procedure. However, a simple estimate of this total count can be made; the total number of C cells in every tenth section (6 μm) of thyroid was found to be highly correlated with the weight of the animal expressed as an allometric function. A better estimate can be derived from counts of just three sections: the tenth, twentieth, and thirtieth after the section of greatest cross sectional area.
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jmor.1051860304
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