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  • 1
    ISSN: 0730-2312
    Keywords: human oocytes ; immunogold labeling ; splicing factors ; coilin ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: The distribution of two splicing components (snRNP and SC-35) and coilin were studied by immunogold/electron microscopy in human oocytes from antral follicles at different levels of transcriptional activity (i.e., active, intermediate, and inactive). The results showed a decrease of snRNPs and SC-35 in the karyoplasm as the oocytes progress from a transcriptionally active to the inactive state. The main areas of accumulation of both these splicing components in all stages of oocytes appeared to be the interchromatin granule clusters (IGCs). Within the IGCs, the two splicing components seemed to be spatially segregated, with the snRNPs predominantly bound to the fibrillar region, whereas the SC-35 factors are being enriched in the granular zone. The p80 coilin was found only in the nucleolus-like body (NLB), which is present in all three stages of oocytes; no coiled bodies were evident. These data are consistent with the notion that splicing occurs in the karyoplasm and that the splicing components are mobilized from a storage site (IGCs) to the site of action. J. Cell. Biochem. 69:72-80, 1998. © 1998 Wiley-Liss, Inc.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 0148-7280
    Keywords: human oocyte nucleus ; karyosphere ; RNA synthesis ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: The functional organization of the nucleus in the oocytes from human antral follicles was examined by morphological and autoradiographic analysis methods at the light and electron microscopic level. According to the position of the nucleus, the level of its transcriptional activity, and the pattern of distribution of structures in it, oocytes fall into two groups. In the first one, the oocytes with the nucleus in the central position are characterized by the distribution of numerous structures all over the nucleus or by a different extent of aggregation of chromatin around the nucleolus. The nuclei of these oocytes are characterized by [3H]uridine incorporation, the label being localized over purely fibrillar, agranular nucleoli and over dispersed fibrillar chromatin adjacent to either the regions of densely packed chromatin or fibrillar-granular material of the nucleolus-like bodies. The latter, the same as condensed chromatin, do not incorporate [3H]uridine. In the second group, the nuclei are displaced towards the oocyte's periphery, and chromosomes surround the nucleolus as a continuous mass closely adjacent to its surface, thus forming a karyosphere. The karyosphere formation takes place on the background of cessation of nuclear transcriptional activity. A fully formed karyosphere represents a complex of closely associated inactivated structures: Nucleolus, chromosomes, and nucleolus-like bodies. The karyosphere nucleolus bears no granules and consists of densely packed finely fibrillar material (fibrils 3 nm thick). Two zones (central and peripheral) can be distinguished in a nucleolus. Nucleolus-like bodies, consisting of granules 20 nm in diameter embedded in finely fibrillar material, are often associated with chromosomes.In this study, data obtained by observations on the loss of association between the oocyte (with karyosphere) and corona radiata cells are evaluated. The relation of the karyosphere formation to the atresia process and the duration of karyosphere existence in human antral follicles are also discussed.
    Additional Material: 15 Ill.
    Type of Medium: Electronic Resource
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