Springer Online Journal Archives 1860-2000
Summary Bovine insulin, proinsulin, and intermediate form were prepared by Sephadex gelfiltration and chromatography on DEAE-cellulose. Using a goat anti-insulin serum, the cross-reactions of proinsulin and intermediate form could be shown by immuno-precipitation. A method is described for the separation of proinsulin from insulin in agar gel. New compounds related to proinsulin or insulin were found in human insulin preparations by means of immunoelectrophoresis.
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