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  • 1
    Electronic Resource
    Electronic Resource
    Plasmid-directed mechanisms for bacteriophage defense in lactic streptococci (1987)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 46 (1987), S. 0 
    Details
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Abstract Genetic studies with lactic streptococci have identified a variety of plasmids coding for systems that interfere with phage adsorption, direct restriction and modification activities, and disrupt various stages in the phage lytic cycle. This review describes mechanisms of phage defense that are plasmid-directed in lactic streptococci, examines the physical and genetic properties of the plasmids involved, and discusses genetic strategies for construction of phage-insensitive starter cultures for dairy fermentations.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1574-6968.1987.tb02468.x
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  • 2
    Electronic Resource
    Electronic Resource
    Electrotransformation of Lactobacillus acidophilus Group A1 (1996)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 138 (1996), S. 0 
    Details
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Abstract Two strains of Lactobacillus acidophilus Group A1, the neotype ATCC 4356 and a human isolate NCFM-N2, widely used as a dietary adjunct in milk and cultured dairy products, were transformed with plasmid DNA by electroporation. The transformation characteristics exhibited by the two L. acidophilus strains were found to differ markedly even though they appeared similar at the genomic level based on the DNA patterns of SmaI restriction fragments. To our knowledge, this is the first report of a consistent, reproducible transformation system of Lactobacillus acidophilus strains comprising the A1 DNA homology group.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1574-6968.1996.tb08163.x
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  • 3
    Electronic Resource
    Electronic Resource
    Insertional mutagenesis in Lactococcus lactis subsp. lactis mediated by IS946 (1993)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 107 (1993), S. 0 
    Details
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Abstract Insertional mutagenesis by an IS946-based integration vector (pTRK145) was demonstrated in Lactococcus lactis subsp. lactis. The suicide vector pTRK145 does not replicate in Gram-positive bacteria and, therefore, expression of its erythromycin resistance (Emr) determinant in lactococci requires integration into the bacterial genome. Random integration of pTRK145 in L. lactis MG1363, a Rec+ strain with regions of homology to IS946, was demonstrated by digesting the chromosomal DNA from 20 integrants with EcoRI, which cuts pTRK145 once. Hybridization with a pTRK145-specific probe identified junction fragments which varied in size, indicating random insertion. The utility of pTRK145 was demonstrated by screening integrants for a mutant deficient in maltose utilization (Mal−). A Mal− mutation coincident with pTRK145 integration was isolated. Upon excision of the insert in vivo, the Mal+ Ems phenotype was restored. IS946-based vectors have the potential for development into effective insertional mutagens which may be valuable for localization and cloning of chrosomal genes in lactococci.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1574-6968.1993.tb06001.x
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  • 4
    Electronic Resource
    Electronic Resource
    Marker-free chromosomal integration of the manganese superoxide dismutase gene (sodA) from Streptococcus thermophilus into Lactobacillus gasseri (2005)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 246 (2005), S. 0 
    Details
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: A strategy for functional gene replacement in the chromosome of Lactobacillus gasseri is described. The phospho-β-galactosidase II gene (lacII) was functionally replaced by the manganese superoxide dismutase (MnSOD) gene (sodA) from Streptococcus thermophilus, by adapting the insertional inactivation method described for lactobacilli [Russell, W.M. and Klaenhammer, T.R. 2001 Efficient system for directed integration into the Lactobacillus acidophilus and Lactobacillus gasseri chromosomes via homologous recombination. Appl. Environ. Microbiol. 67, 4361–4364]. L. gasseri carrying the heterologous sodA gene grew on lactose as efficiently as the wild-type parent. An active MnSOD was expressed in the transgenic strain, and the enzyme migrated on PAGE-SOD activity gels to the same position as that of MnSOD from S. thermophilus. The expression of MnSOD from a single copy of sodA integrated in the chromosome of L. gasseri provided enhanced tolerance to hydrogen peroxide, and extended the viability of carbon/energy starved cultures stored at 25 °C. This is the first report showing the successful utilization of the pORI plasmids system to generate marker-free gene integration in L. gasseri strains.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1016/j.femsle.2005.03.044
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  • 5
    Electronic Resource
    Electronic Resource
    Genetics of bacteriocins produced by lactic acid bacteria (1993)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology reviews 12 (1993), S. 0 
    Details
    ISSN: 1574-6976
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Abstract: Lactic acid bacteria produce a variety of bacteriocins that have recently come under detailed investigation. The biochemical and genetic characteristics of these antimicrobial proteins are reviewed and common elements are discussed between the different classes of bacteriocins produced by these Gram-positive bacteria.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1574-6976.1993.tb00012.x
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  • 6
    Electronic Resource
    Electronic Resource
    Genomic features of lactic acid bacteria effecting bioprocessing and health (2005)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology reviews 29 (2005), S. 0 
    Details
    ISSN: 1574-6976
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: The lactic acid bacteria are a functionally related group of organisms known primarily for their bioprocessing roles in food and beverages. More recently, selected members of the lactic acid bacteria have been implicated in a number of probiotic roles that impact general health and well-being. Genomic analyses of multiple members of the lactic acid bacteria, at the genus, species, and strain level, have now elucidated many genetic features that direct their fermentative and probiotic roles. This information is providing an important platform for understanding core mechanisms that control and regulate bacterial growth, survival, signaling, and fermentative processes and, in some cases, potentially underlying probiotic activities within complex microbial and host ecosystems.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1016/j.fmrre.2005.04.007
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  • 7
    Electronic Resource
    Electronic Resource
    Modulation of the microbial ecology of the human colon by probiotics, prebiotics and synbiotics to enhance human health: An overview of enabling science and potential applications (2005)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology ecology 52 (2005), S. 0 
    Details
    ISSN: 1574-6941
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: The application of probiotics and prebiotics to the manipulation of the microbial ecology of the human colon has recently seen many scientific advances. The sequencing of probiotic genomes is providing a wealth of new information on the biology of these microorganisms. In addition, we are learning more about the interactions of probiotics with human cells and with pathogenic bacteria. An alternative means of modulating the colonic microbial community is by the use of prebiotic oligosaccharides. Increasing knowledge of the metabolism of prebiotics by probiotics is allowing us to consider specifically targeting such dietary intervention tools at specific population groups and specific disease states.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1016/j.femsec.2005.01.003
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  • 8
    Electronic Resource
    Electronic Resource
    Identification of the pH-inducible, proton-translocating F1F0-ATPase (atpBEFHAGDC) operon of Lactobacillus acidophilus by differential display: gene structure, cloning and characterization[Paper no. ] (1999)
    Oxford BSL : Blackwell Science Ltd
    Molecular microbiology 33 (1999), S. 0 
    Details
    ISSN: 1365-2958
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Medicine
    Notes: The influence of low pH on inducible gene expression in Lactobacillus acidophilus was investigated by the use of differential display. Logarithmic phase cultures were exposed to pH 3.5 for various intervals, and RNA was isolated and reverse transcribed. The resultant cDNAs were subjected to PCR and the products were resolved by electrophoresis. Several cDNA products were induced after exposure to pH 3.5. One of these products, a 0.7 kb fragment, showed sequence similarity to bacterial atpBEF genes of the atp operon, whose genes encode the various subunits of the F1F0-ATPase. With the 0.7 kb differential display product as a probe, hybridizations with total RNA from untreated and acid-treated L. acidophilus verified the acid inducibility of this operon. The increase in atp mRNA induced by low pH was accompanied by an increase in the activity of the enzyme in membrane extracts. The full-length atp operon was sequenced, and its genes were in the order of atpBEFHAGDC, coding for the a, c, b, δ, α, γ, β and ε subunits respectively. The operon contained no i gene, but was preceded by a 122 bp intergenic space, which contained putative extended −10 and −35 promoter regions. Primer extension analysis of RNA from cultures that were shifted from pH 5.6 to pH 3.5, and held for 0, 30 or 45 min, revealed that the transcriptional start site did not change position as a function of culture pH or time after exposure to pH 3.5. The primary structure and genetic organization indicated that the H+-ATPase of L. acidophilus is a typical F1F0-type ATPase. The similarity to streptococcal ATPases and the acid inducibility of this operon suggest that it may function in the ATP-dependent extrusion of protons and maintenance of cytoplasmic pH. Finally, the use of differential display RT-PCR was an effective approach to identify genes in L. acidophilus induced by an environmental stimulus.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1365-2958.1999.01557.x
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  • 9
    Electronic Resource
    Electronic Resource
    Development of an Expression Strategy Using a Lytic Phage to Trigger Explosive Plasmid Amplification and Gene Expression † (1996)
    [s.l.] : Nature Publishing Company
    Nature biotechnology 14 (1996), S. 82-87 
    Details
    ISSN: 1546-1696
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: [Auszug] A novel plasmid-based expression strategy, exploiting two features of lytic bacteriophages, was developed in Lactococcus lactis. Components of this system include a phage origin of replication and phage expression signals, which were induced to high efficiency upon phage infection of the host. ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/nbt0196-82
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  • 10
    Electronic Resource
    Electronic Resource
    Cloning and expression of the manganese superoxide dismutase gene of Escherichia coli in Lactococcus lactis and Lactobacillus gasseri (1993)
    Springer
    Molecular genetics and genomics 239 (1993), S. 33-40 
    Details
    ISSN: 1617-4623
    Keywords: Escherichia coli ; Superoxide dismutase ; Fusion protein ; Lactococcus ; Lactobacillus
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The Escherichia coli sodA gene encoding the antioxidant enzyme Mn-containing superoxide dismutase (MnSOD), was cloned in the expression vector pMG36e. This vector has a multiple cloning site down-stream of a promoter and Shine-Dalgarno sequences derived from Lactococcus. The protein-coding region of sodA from E. coli was amplified by the polymerase chain reaction, using a thermocycler and Taq DNA polymerase before cloning into pMG36e. When introduced into E. coli, the recombinant plasmid expressed the predicted fusion protein, both in the presence and absence of oxygen. The expression of the fusion protein in E. coli was verified by SOD assays, activity gels and Western blots. The recombinant plasmid was also introduced into Lactococcus lactis, which contains a resident SOD, and into Lactobacillus gasseri, which is devoid of SOD. Transformed lactococci expressed an active SodA fusion protein plus an active hybrid protein composed of subunits of the Lactococcus and the recombinant E. coli enzymes. Transformants of L. gasseri expressed only the fusion SodA protein, which was enzymatically active.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00281598
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