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  • 1
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    Evaluation of antioxidant defense in the various stages of sexual maturation in cultured Sterlet sturgeon (Acipenser ruthenus) (2018)
    Details
    Publication Date: 2021-05-19
    Description: One of the most important defense systems in fish to keep homeostasis is antioxidant defense system. To assay antioxidant enzymes in ovarian maturation process in cultured Sterlet sturgeon Acipenser ruthenus females, 24 fish were identified in four groups: previtellogenic, vitellogenic, post-vitellogenic and atresia (6 in each group) and blood samples were taken. Maturity stages were identified on the basis of histological criteria and direct observation. The activity of catalase (CAT), superoxide dismutase (SOD(, glutathione peroxidase (GPX) and malondialdehyde (MDA) content in blood was investigated. The results showed CAT and GPX activity decreased in the vitellogenic stage in compare to previtellogenic (P 〈0.05), but showed an increase till atresia (P 〈0.05). SOD activity in different stages was not significantly different (P〉 0.05). MDA concentration in different groups did not show significant changes (P〉 0.05). The results of this study showed nevertheless the metabolism increased in the process of sexual maturation, the antioxidant defense is could effectively protect fish against these changes.
    Description: Published
    Keywords: Acipenser ruthenus ; Sterlet sturgeon ; Antioxidant enzymes ; Sturgeon fish ; Blood ; Reproduction ; Lipid peroxidation ; Evaluation ; Sexual ; Maturation
    Repository Name: AquaDocs
    Type: Journal Contribution , Refereed
    Format: pp.131-138
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  • 2
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    Searching the genome of beluga (Huso huso) for sex markers based on targeted bulked segregant analysis (BSA) (2021)
    In:  http://aquaticcommons.org/id/eprint/21740 | 18721 | 2017-11-28 09:11:49 | 21740 | University of Guilan, Faculty of Natural Resources, Iran
    Details
    Publication Date: 2021-06-30
    Description: In sturgeon aquaculture, where the main purpose is caviar production, a reliable method is needed to separate fish according to gender. Currently, due to the lack of external sexual dimorphism, the fish are sexed by an invasive surgical examination of the gonads. Development of a non-invasive procedure for sexing fish based on genetic markers is of special interest. In the present study we employed Bulked Segregant Analysis (BSA) methodology to search for DNA markers associated with the sex of the beluga sturgeon (Huso huso). DNA bulks (male and female) were created by combining equal amounts of genomic DNA from 10 fish of both sexes. A total of 101 decamer primers associated with the sex-specific sequences in non-sturgeon species was used for targeted screening of the bulks, resulting in 2846 bands that all of them were present in both sexes. Our results showed that sex chromosomes are weakly differentiated in the sturgeon genome and comprised sequences not complementary to the sex-specific primers in non-sturgeon species.
    Keywords: Biology ; Fisheries ; genome ; beluga ; huso huso ; sex ; markers ; segregant ; DNA ; species ; Iran
    Repository Name: AquaDocs
    Type: article , TRUE
    Format: application/pdf
    Format: application/pdf
    Format: 185-195
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  • 3
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    Searching the genome of beluga (Huso huso) for sex markers based on targeted Bulked Segregant Analysis (BSA) (2017)
    Details
    Publication Date: 2021-05-19
    Description: In sturgeon aquaculture, where the main purpose is caviar production, a reliable method is needed to separate fish according to gender. Currently, due to the lack of external sexual dimorphism, the fish are sexed by an invasive surgical examination of the gonads. Development of a non-invasive procedure for sexing fish based on genetic markers is of special interest. In the present study we employed Bulked Segregant Analysis (BSA) methodology to search for DNA markers associated with the sex of the beluga sturgeon (Huso huso). DNA bulks (male and female) were created by combining equal amounts of genomic DNA from 10 fish of both sexes. A total of 101 decamer primers associated with the sex-specific sequences in non-sturgeon species was used for targeted screening of the bulks, resulting in 2846 bands that all of them were present in both sexes. Our results showed that sex chromosomes are weakly differentiated in the sturgeon genome and comprised sequences not complementary to the sex-specific primers in non-sturgeon species.
    Description: Published
    Keywords: Huso huso ; Bulked segregant analysis (BSA) ; Beluga ; Genome ; Sex ; Sex markers ; Genetics
    Repository Name: AquaDocs
    Type: Journal Contribution , Refereed
    Format: pp.185-195
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