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  • 1
    Electronic Resource
    Electronic Resource
    Sol-Gel Encapsulated Light-Transducing Protein Phycoerythrin: A New Biomaterial (1995)
    s.l. : American Chemical Society
    Chemistry of materials 7 (1995), S. 1779-1783 
    Details
    ISSN: 1520-5002
    Source: ACS Legacy Archives
    Topics: Chemistry and Pharmacology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1021/cm00058a005
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  • 2
    Electronic Resource
    Electronic Resource
    Preparation of Amylose Derivatives Selectively Modified at C-6. 6-Amino-6-deoxyamylose (1994)
    s.l. : American Chemical Society
    Macromolecules 27 (1994), S. 2917-2922 
    Details
    ISSN: 1520-5835
    Source: ACS Legacy Archives
    Topics: Chemistry and Pharmacology , Physics
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1021/ma00089a004
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  • 3
    Electronic Resource
    Electronic Resource
    Characterization of barnacle (Balanus eburneus and B. cenatus ) adhesive proteins (1997)
    Springer
    Marine biology 127 (1997), S. 629-635 
    Details
    ISSN: 1432-1793
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Cements from two species of barnacles, Balanuseburneus Gould and Balanus crenatus Bruguiére, were analyzed to identify the number and nature of proteins present. B. crenatus cement was composed mainly of a small peptide cross-linked into aggregates ranging in size from ˜ 3 kD to 40–50 kD. These aggregates could be reduced with 2-mercaptoethanol (2-ME) in the presence of sodium dodecyl sulfate (SDS) but only after incubation at 40 °C for 12 to 24 h and only when the cement was recently formed. Reductive alkylation of cysteine residues with 4-vinylpyridine produced a water-soluble peptide of less than 5 kD. By comparison, the cement of B. eburneus could be dissolved only partially in SDS and 2-ME when heated at 100 °C for 10 min. Five major proteins were identified by SDS-PAGE: 7, 22, 36 and 58 kD bands for which N-terminal sequence and amino acid compositions are presented; and a 52 kD band for which sequence data are given. A minor protein band of ˜ 80 kD has the same N-terminus as the 36 kD band. CNBr digests of individual proteins produced peptides for which sequence and composition data are also presented. The study was conducted during 1993 to 1995. In general, the proteins identified from B. crenatus cement are similar to those characterized from B. eburneus, and they are different in composition and sequence than those previously reported from Mytilus edulis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002270050053
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  • 4
    Electronic Resource
    Electronic Resource
    All-optical light modulation in bacteriorhodopsin films (1995)
    Woodbury, NY : American Institute of Physics (AIP)
    Applied Physics Letters 67 (1995), S. 599-601 
    Details
    ISSN: 1077-3118
    Source: AIP Digital Archive
    Topics: Physics
    Notes: We report a convenient method to obtain all-optical light modulation in bacteriorhodopsin films using a degenerate four-wave mixing geometry. Chemically stabilized films of bacteriorhodopsin in a polymer matrix for which the lifetime of the excited M state is tens of seconds were used to demonstrate all-optical light intensity modulation. The films are observed to be stable over a period of 4 years. Due to the sensitivity of the films, small intensities of order microwatt/ cm2 are required in the modulation experiments. Furthermore the fast photochemical transition from M to B permit reasonably fast modulation speeds independent of the slow thermal M and B relaxation time. The experimental system also acts as an all-optical switch where a low power blue pulse turns on a signal red beam. © 1995 American Institute of Physics.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1063/1.115401
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  • 5
    Electronic Resource
    Electronic Resource
    Mirrorless all-optical bistability in bacteriorhodopsin (1993)
    Woodbury, NY : American Institute of Physics (AIP)
    Applied Physics Letters 63 (1993), S. 1489-1491 
    Details
    ISSN: 1077-3118
    Source: AIP Digital Archive
    Topics: Physics
    Notes: We report direct observations of all-optical mirrorless bistability associated with saturable absorption in three kinds of bacteriorhodopsin (BR) samples: wild-type BR in water solution and dispersed in thin films of a polymer matrix as well as water solution of the genetically engineered mutant BRD96N. The experiments are carried out with picosecond pulses at 532 nm. The values measured for the saturation intensity are explained in terms of the relaxation of the excited M state population to the B state of the BR photocycle for the three kinds of samples studied.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1063/1.109665
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  • 6
    Electronic Resource
    Electronic Resource
    Phycobiliproteins encapsulated in sol-gel glass (1996)
    Springer
    Journal of sol gel science and technology 7 (1996), S. 99-108 
    Details
    ISSN: 1573-4846
    Keywords: sol-gel glass ; phycobiliproteins ; biomaterial ; biosensor
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract Light transducing phycobiliproteins are encapsulated in optically transparent sol-gel matrices. Absorption and fluorescence spectroscopies are used to characterize the effect of the sol-gel encapsulation on the conformation and aggregation states of the three major phycobiliproteins present in phycobilisomes: phycoerythrin, phycocyanin, and allophycocyanin. It is found that the effects of sol-gel entrapment on the spectroscopic properties are significantly different for the three phycobiliproteins. The results indicate that phycoerythrin undergoes only minor change in its native structure when entrapped in sol-gel. However, significant changes in conformation and aggregation state occur when phycocyanin and allophycocyanin are entrapped in sol-gel matrices. A thin film of sol-gel encapsulated phycoerythrin is also coated on an optical fiber surface and strong fluorescence from the evanescent wave excitation is detected. The potential applications of sol-gel encapsulated phycobiliproteins in biosensors are discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00401889
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  • 7
    Electronic Resource
    Electronic Resource
    Polymer microsphere and polymer- ferrite nanocomposite preparation by precipitation from water-in-oil microemulsions (1997)
    Springer
    Colloid & polymer science 275 (1997), S. 930-937 
    Details
    ISSN: 1435-1536
    Keywords: Key words Encapsulation ; polyphenol ; w / o microemulsion ; magnetic microspheres
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Abstract  A simple method to encapsulate intramicellar solutes (enzymes, nanoparticles) in polymer microspheres is described. The method involves dissolving a polymer in a minimum amount of solvent. The polymer solution is then added to a large volume of a nonsolvent system containing bis(2-ethylhexyl)sulfo-succinate (AOT) reversed micelles. Immediate precipitation of the dissolved polymer takes place. The precipitation results in the formation of stable microparticles ∼0.1–1 μm in diameter. There is a direct correlation between the internal voidage of the particles and the water content of the micelles. Precipitation also leads to encapsulation of intramicellar solutes, resulting in the formation of microspherical composites.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s003960050168
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  • 8
    Electronic Resource
    Electronic Resource
    Mutagenicity of 2,4,6-trinitrotoluene-surfactant complexes (1982)
    Springer
    Bulletin of environmental contamination and toxicology 28 (1982), S. 33-38 
    Details
    ISSN: 1432-0800
    Source: Springer Online Journal Archives 1860-2000
    Topics: Energy, Environment Protection, Nuclear Power Engineering , Medicine
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01608409
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  • 9
    Electronic Resource
    Electronic Resource
    Control of molecular weight distribution of the biopolymer pullulan produced byAureobasidium pullulans (1993)
    Springer
    Journal of polymers and the environment 1 (1993), S. 3-9 
    Details
    ISSN: 1572-8900
    Keywords: Biopolymer ; pullulan ; polysaccharide ; molecular weight
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology , Energy, Environment Protection, Nuclear Power Engineering , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Abstract The influence of carbon, nitrogen, and phosphate source, along with concentration, was determined for effect on the weight average molecular weight, molecular weight distribution, and yield of pullulan produced byAureobasidium pullulans NRRL-Y 6220. Batch systems, scale-up batch, and continuous fermentations of 1 L and 10 L were also evaluated as were processing variables, including solvents, and extraction conditions. Products with weight average molecular weight from 1.0 × 105 to 4.0 × 106 were produced in 100-g quantities by varying fermentation conditions such as constituents of the culture medium, pH, and length of incubation. Three sets of culture conditions were defined for the formation of low (〈5.0×105), medium (1.0–2.0×106), and high (〉2.0×106) weight average molecular weight polymer. These defined molecular weight fractions of pullulan were used in further studies in producing films and fibers.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01457648
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  • 10
    Electronic Resource
    Electronic Resource
    Molecular weight distribution of chitosan isolated from Mucor rouxii under different culture and processing conditions (1992)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 39 (1992), S. 281-286 
    Details
    ISSN: 0006-3592
    Keywords: chitin ; chitosan ; Mucor rouxii ; polysaccharides ; Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: The isolation of chitosan from a fungal source offers the potential of a product with controlled physicochemical properties not obtainable by the commercial chemical conversion of crustacean chitin. A variety of culture and processing protocols using Mucor rouxii were studied for their effects on biomass yield and chitosan molecular weight. Weight-averaged molecular weight determined by gel permeation chromotography ranged from 2.0 × 105 to approximately 1.4 × 106 daltons. The chitosan yield ranged from 5% to 10% of total biomass dry weight and from 30% to 40% of the cell wall. Of the culture parameters studied, length of incubation and medium composition effected biomass production and molecular weight. Modification of the processing protocol, including the type and strength of acid, and cell wall disruption in acid prior to refluxing were used to optimize the efficiency of chitosan extraction.The degree of deacetylation of fungal and commercial chitosans was compared using infrared spectrometry, titration, and first derivative of UV absorbance spectrometry. The chitosan obtained directly from the fungal cell wall had a higher degree of deacetylation than commercial chitosan from the chemical conversion process.
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bit.260390305
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