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  • 1
    Call number: SR 90.1026(74)
    In: MPE-Report
    Type of Medium: Series available for loan
    Pages: 24 S.
    Series Statement: Max-Planck-Institut für Physik und Astrophysik, Institut für Extraterrestrische Physik 74
    Language: English
    Location: Magazine - must be ordered
    Branch Library: GFZ Library
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  • 2
    Publication Date: 2014-04-03
    Description: Epigenetic regulation of gene expression involves, besides DNA and histone modifications, the relative positioning of DNA sequences within the nucleus. To trace specific DNA sequences in living cells, we used programmable sequence-specific DNA binding of designer transcription activator-like effectors (dTALEs). We designed a recombinant dTALE (msTALE) with variable repeat domains to specifically bind a 19-bp target sequence of major satellite DNA. The msTALE was fused with green fluorescent protein (GFP) and stably expressed in mouse embryonic stem cells. Hybridization with a major satellite probe (3D-fluorescent in situ hybridization) and co-staining for known cellular structures confirmed in vivo binding of the GFP-msTALE to major satellite DNA present at nuclear chromocenters. Dual tracing of major satellite DNA and the replication machinery throughout S-phase showed co-localization during mid to late S-phase, directly demonstrating the late replication timing of major satellite DNA. Fluorescence bleaching experiments indicated a relatively stable but still dynamic binding, with mean residence times in the range of minutes. Fluorescently labeled dTALEs open new perspectives to target and trace DNA sequences and to monitor dynamic changes in subnuclear positioning as well as interactions with functional nuclear structures during cell cycle progression and cellular differentiation.
    Keywords: Chromatin and Epigenetics
    Print ISSN: 0305-1048
    Electronic ISSN: 1362-4962
    Topics: Biology
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  • 3
    Publication Date: 2022-01-31
    Description: Emotion is a defining aspect of the human condition. Emotions pervade our social and professional lives, they affect our thinking and behavior, and they profoundly shape our relationships and social interactions. Emotions have traditionally been conceptualized and studied as individual phenomena, with research focusing on cognitive and expressive components and on physiological and neurological processes underlying emotional reactions. Over the last two decades, however, an increasing scholarly awareness has emerged that emotions are inherently social – that is, they tend to be elicited by other people, expressed at other people, and regulated to influence other people or to comply with social norms (Fischer & Manstead, 2008; Keltner & Haidt, 1999; Parkinson, 1996; Van Kleef, 2009). Despite this increasing awareness, the inclusion of the social dimension as a fundamental element in emotion research is still in its infancy (Fischer & Van Kleef, 2010). We therefore organized this special Research Topic on the social nature of emotions to review the state of the art in research and methodology and to stimulate theorizing and future research. The emerging field of research into the social nature of emotions has focused on three broad sets of questions. The first set of questions pertains to how social-contextual factors shape the experience, regulation, and expression of emotions. Studies have shown, for instance, that the social context influences the emotions people feel and express (Clark, Fitness, & Brissette, 2004; Doosje, Branscombe, Spears, & Manstead, 2004; Fischer & Evers, 2011). The second set of questions concerns social-contextual influences on the recognition and interpretation of emotional expressions. Studies have shown that facial expressions are interpreted quite differently depending on the social context (e.g., in terms of status, culture, or gender) in which they are expressed (Elfenbein & Ambady, 2002; Hess & Fischer, 2013; Mesquita & Markus, 2004; Tiedens, 2001). The third set of questions has to do with the ways in which people respond to the emotional expressions of others, and how such responses are shaped by the social context. Studies have shown that emotional expressions can influence the behavior of others, for instance in group settings (Barsade, 2002; Cheshin, Rafaeli & Bos, 2011; Heerdink, Van Kleef, Homan, & Fischer, 2013), negotiations (Sinaceur & Tiedens, 2006; Van Kleef, De Dreu, & Manstead, 2004), and leadership (Sy, Côté, & Saavedra, 2005; Van Kleef, Homan, Beersma, & Van Knippenberg, 2010). This Research Topic centers around these and related questions regarding the social nature of emotions, thereby highlighting new research opportunities and guiding future directions in the field. We bring together a collection of papers to provide an encyclopedic, open-access snapshot of the current state of the art of theorizing and research on the social nature of emotion. The state of the art work that is presented in this e-book helps advance the understanding of the social nature of emotions. It brings together the latest cutting-edge findings and thoughts on this central topic in emotion science, as it heads toward the next frontier.
    Keywords: BF1-990 ; Q1-390 ; emotion processing ; emotion ; affective science ; Group processes ; culture ; emotional expression ; social interaction ; Interpersonal Relations
    Language: English
    Format: image/jpeg
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  • 4
    Publication Date: 2016-08-03
    Description: An updated version of the Heated Condensation Framework (HCF) is implemented as a convective triggering criterion into the National Centers for Environmental Prediction (NCEP) Climate Forecast System version 2 (CFSv2). The new trigger replaces the original criteria in both the deep (Simplified Arakawa-Schubert – SAS) and shallow (SAS based) convective schemes. The performance of the original and new triggering criteria is first compared against radiosonde observations. Then, a series of hindcasts are performed to evaluate the influence of the triggering criterion in the CFSv2 representation of summer precipitation, the diurnal cycle of precipitation, and hurricanes that made landfall. The observational analysis shows that the HCF trigger better captures the frequency of convection, where the original SAS trigger initiates convection too often. When implemented in CFSv2, the HCF trigger improves the seasonal forecast of the Indian summer monsoon rainfall, including the representation of the onset dates of the rainy season over India. On the other hand, the HCF trigger increases error in the seasonal forecast of precipitation over the eastern United States. The HCF trigger also improves the representation of the intensity of hurricanes. Moreover, the simulation of hurricanes provides insights on the mechanism whereby the HCF trigger impacts the representation of convection. This article is protected by copyright. All rights reserved.
    Electronic ISSN: 1942-2466
    Topics: Geography , Geosciences
    Published by Wiley on behalf of American Geophysical Union (AGU).
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  • 5
    Publication Date: 2013-03-29
    Description: Author(s): R. Hubele, A. LaForge, M. Schulz, J. Goullon, X. Wang, B. Najjari, N. Ferreira, M. Grieser, V. L. B. de Jesus, R. Moshammer, K. Schneider, A. B. Voitkiv, and D. Fischer We present initial-state selective fully differential cross sections for ionization of lithium by 24 MeV O 8+ impact. The data for ionization from the 2 s and 2 p states look qualitatively different from each other and from 1 s ionization of He. For ionization from the 2 p state, to which in our study th... [Phys. Rev. Lett. 110, 133201] Published Thu Mar 28, 2013
    Keywords: Atomic, Molecular, and Optical Physics
    Print ISSN: 0031-9007
    Electronic ISSN: 1079-7114
    Topics: Physics
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  • 6
    Publication Date: 2013-09-13
    Description: The mountains of data thrusting from the new landscape of modern high-throughput biology are irrevocably changing biomedical research and creating a near-insatiable demand for training in data management and manipulation and data mining and analysis. Among life scientists, from clinicians to environmental researchers, a common theme is the need not just to use, and gain familiarity with, bioinformatics tools and resources but also to understand their underlying fundamental theoretical and practical concepts. Providing bioinformatics training to empower life scientists to handle and analyse their data efficiently, and progress their research, is a challenge across the globe. Delivering good training goes beyond traditional lectures and resource-centric demos, using interactivity, problem-solving exercises and cooperative learning to substantially enhance training quality and learning outcomes. In this context, this article discusses various pragmatic criteria for identifying training needs and learning objectives, for selecting suitable trainees and trainers, for developing and maintaining training skills and evaluating training quality. Adherence to these criteria may help not only to guide course organizers and trainers on the path towards bioinformatics training excellence but, importantly, also to improve the training experience for life scientists.
    Print ISSN: 1467-5463
    Electronic ISSN: 1477-4054
    Topics: Biology , Computer Science
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  • 7
    Publication Date: 2015-10-23
    Description: High-temperature requirement A1 (HTRA1) is a secreted serine protease reported to play a role in the development of several cancers and neurodegenerative diseases. Still, the mechanism underlying the disease processes largely remains undetermined. In age-related macular degeneration (AMD), a common cause of vision impairment and blindness in industrialized societies, two synonymous polymorphisms (rs1049331:C〉T, and rs2293870:G〉T) in exon 1 of the HTRA1 gene were associated with a high risk to develop disease. Here, we show that the two polymorphisms result in a protein with altered thermophoretic properties upon heat-induced unfolding, trypsin accessibility and secretion behavior, suggesting unique structural features of the AMD-risk-associated HTRA1 protein. Applying MicroScale Thermophoresis and protease digestion analysis, we demonstrate direct binding and proteolysis of transforming growth factor β1 (TGF-β1) by normal HTRA1 but not the AMD-risk-associated isoform. As a consequence, both HTRA1 isoforms strongly differed in their ability to control TGF-β mediated signaling, as revealed by reporter assays targeting the TGF-β1-induced serpin peptidase inhibitor (SERPINE1, alias PAI-1) promoter. In addition, structurally altered HTRA1 led to an impaired autocrine TGF-β signaling in microglia, as measured by a strong down-regulation of downstream effectors of the TGF-β cascade such as phosphorylated SMAD2 and PAI-1 expression. Taken together, our findings demonstrate the effects of two synonymous HTRA1 variants on protein structure and protein interaction with TGF-β1. As a consequence, this leads to an impairment of TGF-β signaling and microglial regulation. Functional implications of the altered properties on AMD pathogenesis remain to be clarified.
    Print ISSN: 0964-6906
    Electronic ISSN: 1460-2083
    Topics: Biology , Medicine
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  • 8
    Publication Date: 2015-07-02
    Description: A mineral intermediate between sillimanite and mullite, tentatively designated as "sillimullite," was studied by electron microprobe analyses and single-crystal X-ray diffraction methods. The chemical compositions derived from the microprobe results and the crystal-structure refinement are Al 7.84 Fe 0.18 Ti 0.03 Mg 0.03 Si 3.92 O 19.96 and Al 8.28 Fe 0.20 Si 3.52 O 19.76 (Fe is Fe 3+ ) corresponding to x -values of 0.02 and 0.12, respectively, in the solid-solution series Al 8+4x Si 4–4x O 20–2x assigning Fe 3+ , Ti, and Mg to the Al site. The composition derived from microprobe analysis is very close to a stoichiometric sillimanite (with Fe 3+ ,Ti, and Mg assigned to Al sites), while the composition derived from diffraction data is midway between sillimanite and Si-rich mullites. The discrepancy is assumed to be caused by the occurrence of amorphous nano-sized SiO 2 inclusions in the aluminosilicate phase not affecting the diffraction data but detected in the microprobe analysis. "Sillimullite" crystallizes in the orthorhombic space group Pnam with a = 7.5127(4), b = 7.6823(4), c = 5.785(3) Å, V = 333.88(4) Å 3 , Z = 1. It has a complete Si/Al ordering at tetrahedral sites like sillimanite but with neighboring double chains of SiO 4 and AlO 4 tetrahedra being offset by 1/2 unit cell parallel to c relative to each other causing the change of the space-group setting from Pbnm (sillimanite) to Pnam . Difference Fourier calculations and refinements with anisotropic displacement parameters revealed the formation of oxygen vacancies and triclusters as known in the crystal structures of mullite. Final refinements converged at R 1 = 5.9% for 1024 unique reflections with F o 〉 4( F o ). Fe was found to reside predominantly in the octahedral site and with minor amounts in one of the T* sites. Mg and Ti were not considered in the refinements. The crystal studied here is considered to represent a new mineral intermediate between sillimanite and mullite, named "sillimullite."
    Print ISSN: 0003-004X
    Electronic ISSN: 1945-3027
    Topics: Geosciences
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  • 9
    Publication Date: 2016-01-21
    Description: Author(s): T. Engels, D. Kolomenskiy, K. Schneider, F.-O. Lehmann, and J. Sesterhenn A simulation of a flying bee shows that insects don’t expend extra energy to maintain lift in turbulent air flow. [Phys. Rev. Lett. 116, 028103] Published Fri Jan 15, 2016
    Keywords: Polymer, Soft Matter, Biological, and Interdisciplinary Physics
    Print ISSN: 0031-9007
    Electronic ISSN: 1079-7114
    Topics: Physics
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  • 10
    Publication Date: 2016-03-01
    Description: Biochemistry DOI: 10.1021/acs.biochem.5b01278
    Print ISSN: 0006-2960
    Electronic ISSN: 1520-4995
    Topics: Biology , Chemistry and Pharmacology
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