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  • 1
    Publication Date: 2016-01-08
    Print ISSN: 0895-0695
    Electronic ISSN: 1938-2057
    Topics: Geosciences
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  • 2
    Publication Date: 2016-01-06
    Description: A diffusion model for the coordination of DNA replication in 〈i〉Schizosaccharomyces pombe〈/i〉 Scientific Reports, Published online: 5 January 2016; doi:10.1038/srep18757
    Electronic ISSN: 2045-2322
    Topics: Natural Sciences in General
    Published by Springer Nature
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  • 3
    Publication Date: 2019
    Electronic ISSN: 2397-3366
    Topics: Physics
    Published by Springer Nature
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  • 4
    Publication Date: 2011-06-23
    Description: Author(s): Mahmoud Bakr, R. Kinjo, Y. W. Choi, M. Omer, K. Yoshida, S. Ueda, M. Takasaki, K. Ishida, N. Kimura, T. Sonobe, T. Kii, K. Masuda, H. Ohgaki, and H. Zen The back bombardment (BB) effect limits wide usage of thermionic rf guns. The BB effect induces not only ramping-up of a cathode’s temperature and beam current, but also degradation of cavity voltage and beam energy during a macropulse. This paper presents a comparison of the BB effect for the case ... [Phys. Rev. ST Accel. Beams 14, 060708] Published Wed Jun 22, 2011
    Keywords: Synchrotron Radiation and Free-Electron Lasers
    Electronic ISSN: 1098-4402
    Topics: Physics
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  • 5
    Publication Date: 2015-04-28
    Description: Lactosylceramide (LacCer), which is essential for many cellular processes, is highly expressed on the plasma membranes of human neutrophils and mediates innate immune functions. Less is known, however, about the properties and biological functions of LacCer in mouse neutrophils. This study therefore analyzed the properties of mouse neutrophil LacCer. LacCer was observed on the surface of these cells, with flow cytometry indicating that mouse neutrophil LacCer could be detected by the anti-LacCer mAb T5A7, but not by the anti-LacCer antibodies Huly-m13 and MEM-74. The molecular species of LacCer were nearly identical in mouse and human neutrophils, including C24:0 and C24:1 fatty acid chain-containing species, although the LacCer content in plasma membranes was ~20-fold lower in mouse than in human neutrophils. Surface plasmon resonance analysis revealed that T5A7 bound to a lipid monolayer composed of LacCer, DOPC, cholesterol and sphingomyelin (molar ratio 0.1 : 10 : 10 : 1), whereas Huly-m13 did not. T5A7 induced neutrophil migration, which was abolished by inhibitors of Src-family kinases, PI-3 kinases, and trimeric G (o/i) proteins. T5A7 also inhibited phagocytosis of non-opsonized zymosans by neutrophils. Taken together, these findings suggest that in mouse neutrophils, (i) LacCer is expressed as LacCer-enriched microdomains in cell surface plasma membranes, (ii) these microdomains are recognized by T5A7 but not by other known anti-LacCer antibodies and (iii) LacCer is involved in cell migration and phagocytosis.
    Print ISSN: 0959-6658
    Electronic ISSN: 1460-2423
    Topics: Biology , Medicine
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  • 6
    Publication Date: 2012-04-24
    Description: Class II transfer RNAs (tRNAs), including tRNA Leu and tRNA Ser , have an additional stem and loop structure, the long variable arm (V-arm). Here, we describe Class II tRNAs with a unique anticodon corresponding to neither leucine nor serine. Because these tRNAs are specifically conserved among the nematodes, we have called them ‘nematode-specific V-arm-containing tRNAs’ (nev-tRNAs). The expression of nev-tRNA genes in Caenorhabditis elegans was confirmed experimentally. A comparative sequence analysis suggested that the nev-tRNAs derived phylogenetically from tRNA Leu . In vitro aminoacylation assays showed that nev-tRNA Gly and nev-tRNA Ile are only charged with leucine, which is inconsistent with their anticodons. Furthermore, the deletion and mutation of crucial determinants for leucylation in nev-tRNA led to a marked loss of activity. An in vitro translation analysis showed that nev-tRNA Gly decodes GGG as leucine instead of the universal glycine code, indicating that nev-tRNAs can be incorporated into ribosomes and participate in protein biosynthesis. Our findings provide the first example of unexpected tRNAs that do not consistently obey the general translation rules for higher eukaryotes.
    Print ISSN: 0305-1048
    Electronic ISSN: 1362-4962
    Topics: Biology
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  • 7
    Publication Date: 2014-08-27
    Description: Tetrapyrroles such as heme and chlorophyll are essential for biological processes, including oxygenation, respiration, and photosynthesis. In the tetrapyrrole biosynthesis pathway, protoporphyrinogen IX oxidase (Protox) catalyzes the formation of protoporphyrin IX, the last common intermediate for the biosynthesis of heme and chlorophyll. Three nonhomologous isofunctional enzymes, HemG, HemJ, and HemY, for Protox have been identified. To reveal the distribution and evolution of the three Protox enzymes, we identified homologs of each along with other heme biosynthetic enzymes by whole-genome clustering across three domains of life. Most organisms possess only one of the three Protox types, with some exceptions. Detailed phylogenetic analysis revealed that HemG is mostly limited to -Proteobacteria whereas HemJ may have originated within α-Proteobacteria and transferred to other Proteobacteria and Cyanobacteria. In contrast, HemY is ubiquitous in prokaryotes and is the only Protox in eukaryotes, so this type may be the ancestral Protox. Land plants have a unique HemY homolog that is also shared by Chloroflexus species, in addition to the main HemY homolog originating from Cyanobacteria. Meanwhile, organisms missing any Protox can be classified into two groups; those lacking most heme synthetic genes, which necessarily depend on external heme supply, and those lacking only genes involved in the conversion of uroporphyrinogen III into heme, which would use a precorrin2-dependent alternative pathway. However, hemN encoding coproporphyrinogen IX oxidase was frequently found in organisms lacking Protox enzyme, which suggests a unique role of this gene other than in heme biosynthesis.
    Electronic ISSN: 1759-6653
    Topics: Biology
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  • 8
    Publication Date: 2008-04-11
    Description: During haematopoiesis, pluripotent haematopoietic stem cells are sequentially restricted to give rise to a variety of lineage-committed progenitors. The classical model of haematopoiesis postulates that, in the first step of differentiation, the stem cell generates common myelo-erythroid progenitors and common lymphoid progenitors (CLPs). However, our previous studies in fetal mice showed that myeloid potential persists even as the lineage branches segregate towards T and B cells. We therefore proposed the 'myeloid-based' model of haematopoiesis, in which the stem cell initially generates common myelo-erythroid progenitors and common myelo-lymphoid progenitors. T-cell and B-cell progenitors subsequently arise from common myelo-lymphoid progenitors through myeloid-T and myeloid-B stages, respectively. However, it has been unclear whether this myeloid-based model is also valid for adult haematopoiesis. Here we provide clonal evidence that the early cell populations in the adult thymus contain progenitors that have lost the potential to generate B cells but retain substantial macrophage potential as well as T-cell, natural killer (NK)-cell and dendritic-cell potential. We also show that such T-cell progenitors can give rise to macrophages in the thymic environment in vivo. Our findings argue against the classical dichotomy model in which T cells are derived from CLPs; instead, they support the validity of the myeloid-based model for both adult and fetal haematopoiesis.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Wada, Haruka -- Masuda, Kyoko -- Satoh, Rumi -- Kakugawa, Kiyokazu -- Ikawa, Tomokatsu -- Katsura, Yoshimoto -- Kawamoto, Hiroshi -- England -- Nature. 2008 Apr 10;452(7188):768-72. doi: 10.1038/nature06839.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Laboratory for Lymphocyte Development, RIKEN Research Center for Allergy and Immunology, Yokohama 230-0045, Japan.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/18401412" target="_blank"〉PubMed〈/a〉
    Keywords: Aging/*physiology ; Animals ; B-Lymphocytes/cytology ; *Cell Lineage ; Cells, Cultured ; Coculture Techniques ; Dendritic Cells/cytology ; Fetus ; *Hematopoiesis ; Hematopoietic Stem Cells/*cytology/metabolism ; Killer Cells, Natural/cytology ; Macrophages/cytology/metabolism ; Mice ; Models, Biological ; Myeloid Cells/*cytology/metabolism ; Stromal Cells/cytology ; T-Lymphocytes/*cytology/metabolism ; Thymus Gland/cytology/embryology/transplantation
    Print ISSN: 0028-0836
    Electronic ISSN: 1476-4687
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
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  • 9
    Publication Date: 2008-02-16
    Description: Searches for extrasolar planets have uncovered an astonishing diversity of planetary systems, yet the frequency of solar system analogs remains unknown. The gravitational microlensing planet search method is potentially sensitive to multiple-planet systems containing analogs of all the solar system planets except Mercury. We report the detection of a multiple-planet system with microlensing. We identify two planets with masses of approximately 0.71 and approximately 0.27 times the mass of Jupiter and orbital separations of approximately 2.3 and approximately 4.6 astronomical units orbiting a primary star of mass approximately 0.50 solar mass at a distance of approximately 1.5 kiloparsecs. This system resembles a scaled version of our solar system in that the mass ratio, separation ratio, and equilibrium temperatures of the planets are similar to those of Jupiter and Saturn. These planets could not have been detected with other techniques; their discovery from only six confirmed microlensing planet detections suggests that solar system analogs may be common.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Gaudi, B S -- Bennett, D P -- Udalski, A -- Gould, A -- Christie, G W -- Maoz, D -- Dong, S -- McCormick, J -- Szymanski, M K -- Tristram, P J -- Nikolaev, S -- Paczynski, B -- Kubiak, M -- Pietrzynski, G -- Soszynski, I -- Szewczyk, O -- Ulaczyk, K -- Wyrzykowski, L -- OGLE Collaboration -- Depoy, D L -- Han, C -- Kaspi, S -- Lee, C-U -- Mallia, F -- Natusch, T -- Pogge, R W -- Park, B-G -- MuFUN Collaboration -- Abe, F -- Bond, I A -- Botzler, C S -- Fukui, A -- Hearnshaw, J B -- Itow, Y -- Kamiya, K -- Korpela, A V -- Kilmartin, P M -- Lin, W -- Masuda, K -- Matsubara, Y -- Motomura, M -- Muraki, Y -- Nakamura, S -- Okumura, T -- Ohnishi, K -- Rattenbury, N J -- Sako, T -- Saito, To -- Sato, S -- Skuljan, L -- Sullivan, D J -- Sumi, T -- Sweatman, W L -- Yock, P C M -- MOA Collaboration -- Albrow, M D -- Allan, A -- Beaulieu, J-P -- Burgdorf, M J -- Cook, K H -- Coutures, C -- Dominik, M -- Dieters, S -- Fouque, P -- Greenhill, J -- Horne, K -- Steele, I -- Tsapras, Y -- PLANET and RoboNet Collaborations -- Chaboyer, B -- Crocker, A -- Frank, S -- Macintosh, B -- New York, N.Y. -- Science. 2008 Feb 15;319(5865):927-30. doi: 10.1126/science.1151947.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Astronomy, Ohio State University, 140 West 18th Avenue, Columbus, OH 43210, USA. gaudi@astronomy.ohio-state.edu〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/18276883" target="_blank"〉PubMed〈/a〉
    Print ISSN: 0036-8075
    Electronic ISSN: 1095-9203
    Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics
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  • 10
    Publication Date: 2004-08-31
    Description: Observations of the gravitational microlensing event MOA 2003-BLG-32/OGLE 2003-BLG-219 are presented, for which the peak magnification was over 500, the highest yet reported. Continuous observations around the peak enabled a sensitive search for planets orbiting the lens star. No planets were detected. Planets 1.3 times heavier than Earth were excluded from more than 50% of the projected annular region from approximately 2.3 to 3.6 astronomical units surrounding the lens star, Uranus-mass planets were excluded from 0.9 to 8.7 astronomical units, and planets 1.3 times heavier than Saturn were excluded from 0.2 to 60 astronomical units. These are the largest regions of sensitivity yet achieved in searches for extrasolar planets orbiting any star.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Abe, F -- Bennett, D P -- Bond, I A -- Eguchi, S -- Furuta, Y -- Hearnshaw, J B -- Kamiya, K -- Kilmartin, P M -- Kurata, Y -- Masuda, K -- Matsubara, Y -- Muraki, Y -- Noda, S -- Okajima, K -- Rakich, A -- Rattenbury, N J -- Sako, T -- Sekiguchi, T -- Sullivan, D J -- Sumi, T -- Tristram, P J -- Yanagisawa, T -- Yock, P C M -- Gal-Yam, A -- Lipkin, Y -- Maoz, D -- Ofek, E O -- Udalski, A -- Szewczyk, O -- Zebrun, K -- Soszynski, I -- Szymanski, M K -- Kubiak, M -- Pietrzynski, G -- Wyrzykowski, L -- New York, N.Y. -- Science. 2004 Aug 27;305(5688):1264-6.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Solar Terrestrial Environment Laboratory, Nagoya University, Nagoya 464-01, Japan.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/15333833" target="_blank"〉PubMed〈/a〉
    Print ISSN: 0036-8075
    Electronic ISSN: 1095-9203
    Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics
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