Publication Date:
2014-09-13
Description:
Production of healthy gametes requires a reductional meiosis I division in which replicated sister chromatids comigrate, rather than separate as in mitosis or meiosis II. Fusion of sister kinetochores during meiosis I may underlie sister chromatid comigration in diverse organisms, but direct evidence for such fusion has been lacking. We used laser trapping and quantitative fluorescence microscopy to study native kinetochore particles isolated from yeast. Meiosis I kinetochores formed stronger attachments and carried more microtubule-binding elements than kinetochores isolated from cells in mitosis or meiosis II. The meiosis I-specific monopolin complex was both necessary and sufficient to drive these modifications. Thus, kinetochore fusion directs sister chromatid comigration, a conserved feature of meiosis that is fundamental to Mendelian inheritance.〈br /〉〈br /〉〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4226495/" target="_blank"〉〈img src="https://static.pubmed.gov/portal/portal3rc.fcgi/4089621/img/3977009" border="0"〉〈/a〉 〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4226495/" target="_blank"〉This paper as free author manuscript - peer-reviewed and accepted for publication〈/a〉〈br /〉〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Sarangapani, Krishna K -- Duro, Eris -- Deng, Yi -- Alves, Flavia de Lima -- Ye, Qiaozhen -- Opoku, Kwaku N -- Ceto, Steven -- Rappsilber, Juri -- Corbett, Kevin D -- Biggins, Sue -- Marston, Adele L -- Asbury, Charles L -- 077707/Wellcome Trust/United Kingdom -- 084229/Wellcome Trust/United Kingdom -- 090903/Wellcome Trust/United Kingdom -- 091020/Wellcome Trust/United Kingdom -- 092076/Wellcome Trust/United Kingdom -- 096078/Wellcome Trust/United Kingdom -- P30 CA015704/CA/NCI NIH HHS/ -- R01 GM079373/GM/NIGMS NIH HHS/ -- R01 GM104141/GM/NIGMS NIH HHS/ -- R01GM064386/GM/NIGMS NIH HHS/ -- R01GM079373/GM/NIGMS NIH HHS/ -- R01GM104141/GM/NIGMS NIH HHS/ -- S10 RR026406/RR/NCRR NIH HHS/ -- S10RR026406/RR/NCRR NIH HHS/ -- New York, N.Y. -- Science. 2014 Oct 10;346(6206):248-51. doi: 10.1126/science.1256729. Epub 2014 Sep 11.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Physiology and Biophysics, University of Washington, Seattle, WA 98195, USA. ; Wellcome Trust Centre for Cell Biology, University of Edinburgh, Edinburgh, UK. ; Ludwig Institute for Cancer Research, San Diego Branch, La Jolla, CA 92093, USA. ; Division of Basic Sciences, Fred Hutchinson Cancer Research Center, Seattle, WA 98109, USA. ; Wellcome Trust Centre for Cell Biology, University of Edinburgh, Edinburgh, UK. Institute of Bioanalytics, Department of Biotechnology, Technische Universitat Berlin, Berlin, Germany. ; Ludwig Institute for Cancer Research, San Diego Branch, La Jolla, CA 92093, USA. Department of Cellular and Molecular Medicine, University of California, San Diego, La Jolla, CA 92093, USA. ; Division of Basic Sciences, Fred Hutchinson Cancer Research Center, Seattle, WA 98109, USA. Department of Biochemistry, University of Washington, Seattle, WA 98195, USA. ; Wellcome Trust Centre for Cell Biology, University of Edinburgh, Edinburgh, UK. casbury@u.washington.edu adele.marston@ed.ac.uk. ; Department of Physiology and Biophysics, University of Washington, Seattle, WA 98195, USA. casbury@u.washington.edu adele.marston@ed.ac.uk.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/25213378" target="_blank"〉PubMed〈/a〉
Keywords:
Casein Kinase I/genetics/metabolism
;
Cell Cycle Proteins/genetics/metabolism
;
Chromatids/metabolism
;
Kinetochores/*metabolism
;
*Meiosis
;
Microscopy, Fluorescence
;
Nuclear Proteins/genetics/metabolism
;
Optical Tweezers
;
Saccharomyces cerevisiae/*cytology/*metabolism
;
Saccharomyces cerevisiae Proteins/genetics/metabolism
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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