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  • 1
    Electronic Resource
    Electronic Resource
    Somatic hybrids produced between Lycopersicon esculentum and L. hirsutum
    Amsterdam : Elsevier
    Plant Science 91 (1993), S. 55-65 
    Details
    ISSN: 0168-9452
    Keywords: RFLP ; allotetraploids ; disomic polyploids ; organelle DNA ; somatic hybridization ; tomato
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology
    Type of Medium: Electronic Resource
    URL: http://linkinghub.elsevier.com/retrieve/pii/0168-9452(93)90188-6
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  • 2
    Electronic Resource
    Electronic Resource
    Plant regeneration from leaf protoplasts of Lycopersicon hirsutum f. hirsutum (1991)
    Springer
    Plant cell reports 9 (1991), S. 680-683 
    Details
    ISSN: 1432-203X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A protocol is described for high frequency plant regeneration from isolated leaf protoplasts of several genotypes of the wild tomato species Lycopersicon hirsutum f. hirsutum based on modified tomato protoplast culture methods. Three to four week old seedlings exposed to a low light pretreatment yielded protoplasts capable of sustained divisions on modified Lycopersicon Culture Medium. Plating efficiencies varied from 7.2%–25.9%. Colonies were transferred to modified solid greening medium after 25–35 days. Developing calli that turned bright green and produced dark green bud primordia were transferred to shoot induction medium. Shoot formation efficiencies ranged from 60%–85%. Shoots rooted easily and regenerated plants grown to complete maturity showed only transient somaclonal variation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00235356
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  • 3
    Electronic Resource
    Electronic Resource
    Levels of indole-3-acetic acid in intact and decapitated coleoptiles as determined by a specific and highly sensitive solid-phase enzyme immunoassay (1981)
    Springer
    Planta 153 (1981), S. 561-571 
    Details
    ISSN: 1432-2048
    Keywords: Auxin (immunoassay) ; Avena ; Coleoptiles (physiological tip) ; Enzyme immunoassay ; Zea
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A specific solid-phase enzyme immunoassay for the detection of as little as 3–4 pg of indole-3-acetic acid (IAA) is described. The assay involves minimal procedural efforts and requires only standard laboratory equipment. Up to 50 samples in triplicate, processed simultaneously, can be assayed and evaluated in 2.5 h. As little as 1 mg oat coleoptile tissue is sufficient for a quantitative IAA analysis and little or no extract purification is necessary. Using this assay, levels of IAA have been determined in coleoptiles of maize and oat. The distribution of IAA within single coleoptiles was quantitated and the production of IAA during the regeneration of the physiological tip in Avena coleoptiles was investigated. The changes in levels of IAA and other major phytohormones were quantitated during the growth of oat coleoptiles.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00385542
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  • 4
    Electronic Resource
    Electronic Resource
    Synthesis of male sterile, triazine-resistant Brassica napus by somatic hybridization between cytoplasmic male sterile B. oleracea and atrazine-resistant B. campestris (1989)
    Springer
    Theoretical and applied genetics 78 (1989), S. 445-455 
    Details
    ISSN: 1432-2242
    Keywords: Brassica napus ; Protoplast fusion ; Triazine resistance ; Cytoplasmic male sterility ; Mitochondrial DNA
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Fusion of leaf protoplasts from an inbred line of Brassica oleracea ssp. botrytis (cauliflower, n=9) carrying the Ogura (R1) male sterile cytoplasm with hypocotyl protoplasts of B. campestris ssp. oleifera (cv “Candle”, n=10) carrying an atrazine-resistant (ATR) cytoplasm resulted in the production of synthetic B. napus (n=19). Thirty-four somatic hybrids were produced; they were characterized for morphology, phosphoglucose isomerase isoenzymes, ribosomal DNA hybridization patterns, chromosome numbers, and organelle composition. All somatic hybrids carried atrazine-resistant chloroplasts derived from B. campestris. The mitochondrial genomes in 19 hybrids were examined by restriction endonuclease and Southern blot analyses. Twelve of the 19 hybrids contained mitochondria showing novel DNA restriction patterns; of these 12 hybrids, 5 were male sterile and 7 were male fertile. The remaining hybrids contained mitochondrial DNA that was identical to that of the ATR parent and all were male fertile.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00265310
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  • 5
    Electronic Resource
    Electronic Resource
    Brassica carinata resynthesized by protoplast fusion (1993)
    Springer
    Theoretical and applied genetics 86 (1993), S. 567-572 
    Details
    ISSN: 1432-2242
    Keywords: Amphidiplids ; Brassica carinata ; Interspecific hybridization ; Protoplast fusion
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Brassica carinata (bbcc) was resynthesized by protoplast fusion betweenB. nigra (bb) andB. oleracea (cc). In two fusion experiments 64 hybrid plants were obtained and identified to be true hybrids by isoenzyme analysis, nuclear DNA content, chromosome number, and intermediate morphology. Of these plants 56% were normal amphidiploids with 2n=34 chromosomes and a DNA content equivalent to that of naturalB. carinata. The remaining plants were polyploid, morphologically abnormal, and infertile. The majority of the hybrids contained both chloroplasts and mitochondria fromB. nigra, but some plants combined chloroplast and mitochondria from the different progenitors. Hybrids with a DNA content equivalent to that ofB. carinata had a wide range of male fertility (4–98%), but consistently low female fertility. Only a few selfed seed were produced, but these germinated and grew into vigorous plants.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00838710
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  • 6
    Electronic Resource
    Electronic Resource
    Atrazine-resistant cauliflower obtained by somatic hybridization between Brassica oleracea and ATR-B. napus (1989)
    Springer
    Theoretical and applied genetics 78 (1989), S. 271-279 
    Details
    ISSN: 1432-2242
    Keywords: Brassica oleracea ; Protoplast fusion ; Herbicide resistance ; Cytoplasmic traits
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Somatic hybridization between Brassica oleracea ssp. botrytis (cauliflower, 2n=18), carrying the Ogura (R1) male-sterile cytoplasm and B. napus (2n= 38), carrying a male-fertile, atrazine-resistant (ATR) cytoplasm, yielded three hybrids (2n=56) and six cauliflower cybrids (2n=18), which were selected for resistance to the herbicide in vitro. The hybrids and cybrids were male fertile and self-compatible. They contained both chloroplasts and mitochondria from the ATR cytoplasm. We found no evidence for mtDNA recombination in any of the regenerated plants. Selfed progeny of the B. oleracea atrazine-resistant cybrids were evaluated for tolerance to the herbicide in the field. Resistant plants exposed to 0.56–4.48 kg/ha (0.5–4.0 pounds/acre) atrazine in the soil showed no damage at any herbicide level, whereas plants of a susceptible alloplasmic line were severely damaged at the lowest level of herbicide application and killed at all higher levels. These atrazine-resistant cauliflower may have potential horticultural use, especially in fields where atrazine carry over is a serious problem.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00288810
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  • 7
    Electronic Resource
    Electronic Resource
    Characterization of a partial male fertility derived from Ogura-cms in progeny of a resynthesized Brassica napus (1993)
    Springer
    Sexual plant reproduction 6 (1993), S. 266-274 
    Details
    ISSN: 1432-2145
    Keywords: Brassica ; Cytoplasmic male sterility ; Mitochondria ; Protoplast fusion ; Somatic hybrids
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The inheritance of a partial male fertile phenotype in somatic hybrid B. napus plants that carried novel mtDNA was investigated over five backcross generations to B. napus ‘Triton.’ The recurrent parent and the original somatic hybrid both contained chloroplasts resistant to atrazine. The F1 population contained mainly plants that were partial fertile, and some of the plants differed in mtDNA. The partial fertility predominated in the progeny of each backcross generation, but fully male sterile and fertile plants were also obtained. However, the sterility/fertility of these latter plants was not stable; both the fully male sterile and the male fertile plants produced progeny that were again predominantly partial male fertile. This pattern of predominant partial fertility but occasional sterile and fertile plants persisted in different nuclear backgrounds. Neither the male sterility nor the male fertility could be fixed and made stable. Test crosses indicated that restorer genes were probably not associated with appearance of male fertile plants. The evidence indicates that the behavior of the partial male fertility is cytoplasmic, and probably controlled by the chondriome.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00231904
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  • 8
    Electronic Resource
    Electronic Resource
    Improved protoplast culture and stability of cytoplasmic traits in plants regenerated from leaf protoplasts of cauliflower (Brassica oteracea ssp.botrytis) (1990)
    Springer
    Plant cell, tissue and organ culture 21 (1990), S. 227-236 
    Details
    ISSN: 1573-5044
    Keywords: Brassica oleracea ; protoplasts ; regeneration ; cytoplasmic traits ; mitochondrial DNA
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Nearly 1000 plants have been regenerated from leaf protoplasts of two cauliflower (Brassica oleracea ssp.botrytis) alloplasmic inbred lines. One line (7642A) carried the Ogura (R1) cms cytoplasm derived from radish; the other line (7642B) carried a normalBrassica cytoplasm and was the fertile maintainer for the cms line. The majority of regenerated plants displayed normal vegetative morphology; they formed normal cauliflower heads and retained the floral characteristics of seed-grown plants from which they were derived. We found no change in either male sterility or in the low temperature-induced chlorosis associated with the 7642A line. Mitochondrial DNA analysis by hybridization with five cloned mtDNA probes revealed no apparent alteration in 75 regenerated plants of both lines. These results indicate that cytoplasmic traits inBrassica oleracea are stable after one cycle of in vitro culture and regeneration.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00047615
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  • 9
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    Comparisons between A.C impedance behaviour of graphite hydrogenosulfate and p-doped (CH)x (1989)
    Elsevier
    Details
    Publication Date: 1989-02-01
    Print ISSN: 0379-6779
    Electronic ISSN: 1879-3290
    Topics: Chemistry and Pharmacology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Published by Elsevier
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  • 10
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    Electrointercalation of IIIB metal chlorides MCl4 into polyacetylene (1988)
    Elsevier
    Details
    Publication Date: 1988-04-01
    Print ISSN: 0379-6779
    Electronic ISSN: 1879-3290
    Topics: Chemistry and Pharmacology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Published by Elsevier
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