Publication Date:
2007-06-09
Description:
During cytokinesis, as dividing animal cells pull apart into two daughter cells, the final stage, termed abscission, requires breakage of the midbody, a thin membranous stalk connecting the daughter cells. This membrane fission event topologically resembles the budding of viruses, such as HIV-1, from infected cells. We found that two proteins involved in HIV-1 budding-tumor susceptibility gene 101 (Tsg101), a subunit of the endosomal sorting complex required for transport I (ESCRT-I), and Alix, an ESCRT-associated protein-were recruited to the midbody during cytokinesis by interaction with centrosome protein 55 (Cep55), a centrosome and midbody protein essential for abscission. Tsg101, Alix, and possibly other components of ESCRT-I were required for the completion of cytokinesis. Thus, HIV-1 budding and cytokinesis use a similar subset of cellular components to carry out topologically similar membrane fission events.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Carlton, Jez G -- Martin-Serrano, Juan -- G0400207/Medical Research Council/United Kingdom -- New York, N.Y. -- Science. 2007 Jun 29;316(5833):1908-12. Epub 2007 Jun 7.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Infectious Diseases, King's College London School of Medicine, London, UK.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/17556548" target="_blank"〉PubMed〈/a〉
Keywords:
Adenosine Triphosphatases/metabolism
;
Amino Acid Motifs
;
Calcium-Binding Proteins/*metabolism
;
Carrier Proteins/*metabolism
;
Cell Cycle Proteins/*metabolism
;
*Cytokinesis
;
DNA-Binding Proteins/*metabolism
;
Endosomal Sorting Complexes Required for Transport
;
Endosomes/metabolism
;
HIV-1/*physiology
;
HeLa Cells
;
Humans
;
Microtubules/metabolism
;
Nuclear Proteins/*metabolism
;
Protein Binding
;
Protein Structure, Tertiary
;
R-SNARE Proteins/metabolism
;
RNA Interference
;
Recombinant Fusion Proteins/metabolism
;
Syntaxin 1/metabolism
;
Transcription Factors/*metabolism
;
Transfection
;
Vesicular Transport Proteins/metabolism
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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