ISSN:
0935-6304
Keywords:
Supercritical fluid chromatography, SFC
;
Capillary columns
;
Chemistry
;
Analytical Chemistry and Spectroscopy
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Chemistry and Pharmacology
Notes:
Supercritical fluid chromatography (SFC) sometimes exhibits GC-like behavior and sometimes LC-like behavior, depending on conditions. However, it is not always clear whether one of these types of behavior, or a combination, operates for a particular set of conditions for every solute in a mixture. For example, some components may be partitioned mostly by their vapor pressures, while others, in the same mixture, are partitioned predominantly by solvent-like properties of the mobile phase.Plots of retetion (as log of the capacity factor) vs. reciprocal temperature at constant pressure reveal a clear change in the character of the separation of well-behaved solutes. A thermodynamic explanation of the observed behavior is given, based on the assumption that partitioning is controlled by the heats of solution of solute in the mobile and stationary phases. A model of SFC retention as it deviates from pure-GC behavior on the same column is presented.
Additional Material:
6 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/jhrc.1240080917
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