ISSN:
1662-8985
Source:
Scientific.Net: Materials Science & Technology / Trans Tech Publications Archiv 1984-2008
Topics:
Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
Notes:
The sediment microbial communities of a disused coal mine lake, Lake Kepwari(pH~4.5-5) were studied to understand how the natural microbial processes in an oligotrophic acidicmine lake system influence the iron and sulphur cycles. Most probable number (MPN) viablecounts were used to enumerate the benthic bacteria at different depths. MPN results revealed anabundance of bacteria that were capable of growing in sulphate reducing medium with numbers inthe range of 1 × 107 – 1 × 108 cells.g-1 of wet sediment. In contrast, MPN results showed muchlower numbers of bacteria that were capable of growing in ferric reducing medium with 1 × 102 – 2× 103 cells.g-1 of wet sediment detected. Serial decimal dilution cultures were used to isolate purestrains of benthic bacteria. Strains HP1, HP2 and HP3 were isolated from benthic lake sediments at18 m, 0 m and 10 m water depths respectively. 16S rRNA gene sequence analysis of strain HP1showed that the strain belonged to the genus Enterobacter, strain HP2 belonged to the OrderRhizobiales and strain HP3 belonged to the sub-order Micrococcineae. Denaturing gradient gelelectrophoresis (DGGE) analysis of 16S rRNA gene fragments was used to profile the diversity ofthe benthic microbial communities at different depths. DGGE profiling of benthic sedimentsrevealed that sediments contained mostly members of the Proteobacteria, Actinobacteria andFirmicutes phyla
Type of Medium:
Electronic Resource
URL:
http://www.tib-hannover.de/fulltexts/2011/0528/01/39/transtech_doi~10.4028%252Fwww.scientific.net%252FAMR.20-21.489.pdf
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