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  • 1
    Electronic Resource
    Electronic Resource
    High regeneration rates in anther culture of interspecific sunflower hybrids (1996)
    Springer
    Plant cell reports 16 (1996), S. 167-173 
    Details
    ISSN: 1432-203X
    Keywords: Abbreviations:BAP = 6‐benzylaminopurine; MNR = menadione reductase, NAA =α-naphthalene acetic acid, PGM = phosphoglucomutase, PGI = phosphoglucoisomerase, PVP = polyvinylpyrrolidone, CMS = cytplasmic male sterile, MS = Murashige & Skoog
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract. Optimization of anther culture with regard to the induction of callus formation and direct embryogenesis was obtained for interspecific hybrids of H. annuus with H. tuberosus, H. laetiflorus, and H. resinosus by investigating six different induction media and four regeneration media. One media combination (MS-I3, MS-R3 and MS-R4) used under different culture conditions (30°C/35°C and different dark treatments) gave up to 92.7% embryogenic anthers with an average of 8.5 embryos per anther. However, direct embryogenesis as well as callus formation showed a strong genotypic and treatment specific reaction. From 5,600 anthers of the four investigated genotypes more than 2,000 plants could be regenerated. Regenerants were characterized by morphological traits and isozyme analyses to prove their androgenic origin.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01890860
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  • 2
    Electronic Resource
    Electronic Resource
    High regeneration rates in anther culture of interspecific sunflower hybrids (1996)
    Springer
    Plant cell reports 16 (1996), S. 167-173 
    Details
    ISSN: 1432-203X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Optimization of anther culture with regard to the induction of callus formation and direct embryogenesis was obtained for interspecific hybrids ofH. annuus withH. tuberosus, H. laetiflorus, andH. resinosus by investigating six different induction media and four regeneration media. One media combination (MS-13, MS-R3 and MS-R4) used under different culture conditions (30°C / 35°C and different dark treatments) gave up to 92.7% embryogenic anthers with an average of 8.5 embryos per anther. However, direct embryogenesis as well as callus formation showed a strong genotypec and treatment specific reaction. From 5,600 anthers of the four investigated genotypes more than 2,000 plants could be regenerated. Regenerants were characterized by morphological traits and isozyme analyses to prove their androgenetic origin.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01890860
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  • 3
    Electronic Resource
    Electronic Resource
    High regeneration potential in vitro of sunflower (Helianthus annuus L.) lines derived from interspecific hybridization (2000)
    Springer
    Euphytica 116 (2000), S. 271-280 
    Details
    ISSN: 1573-5060
    Keywords: culture in vitro ; genetic transformation ; Helianthus sp. ; interspecific hybridization ; sunflower ; wild species
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Successful selection of interspecific hybrid progenies with superior ability to regenerate shoots from apical meristems was performed in sunflower which now allows for the development of lines for improved biotechnological applications. Early generations of interspecific hybrids originating from crosses between the two H. annuus CMS lines ‘HA89’ and ‘Baso’, and 9 wild species were screened for their ability to regenerate in vitro. Evaluation of 36 progenies allowed to identify seven progenies from crosses involving H. mollis, H. giganteus, H. strumosus, and H. decapetalus which showed a significantly higher regeneration potential than the commercial hybrid ‘Albena’ regarding the number of shoots per explant. Among these progenies, 47.2 to 62.4% of explants produced shoots with an average of 2.3 to 3.5 shoots per cultured explant. Regeneration in vitro was significantly determined by the genotype. More than half of the investigated interspecific hybrids performed better than the inbred ‘HA89’ demonstrating that the high regeneration potential available in the wild species can be efficiently transferred to cultivated sunflower. The seven progenies with high regeneration potential in vitro were characterised by agronomic performance in the field. Two of the interspecific hybrids derived from H. strumosus and H. decapetalus not only showed a superior regeneration potential but also proved to be competitive to commercial hybrids with regard to important agronomic traits, e.g. fat content and TGW.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1004061632736
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  • 4
    Electronic Resource
    Electronic Resource
    The CMS-associated 16 kDa protein encoded by orfH522 in the PET1 cytoplasm is also present in other male-sterile cytoplasms of sunflower (1996)
    Springer
    Plant molecular biology 30 (1996), S. 523-538 
    Details
    ISSN: 1573-5028
    Keywords: CMS ; helianthus annuus ; in organello translation ; mitochondria ; orfH522 ; sunflower
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract In sunflower plants carrying the PET1 cytoplasm male sterility (CMS) is associated with a new open reading frame (orfH522) in the 3′-flanking region of the atpA gene and an additional 16 kDa protein. Twenty-seven male-sterile cytoplasms of different origin were studied for the expression of the 16 kDa protein. In addition to the PET1 cytoplasm nine other male-sterile cytoplasms express the CMS-associated protein. These CMS sources originate from different interspecific crosses, from spontaneously occurring male-sterile plants in wild sunflower and from induced mutagenesis. Polyclonal antisera were raised against fusion proteins which contain 421 bp of the 3′-coding region of orfH522 to verify by immunological methods the identity of the protein in the other CMS cytoplasms. The anti-ORFH522 antiserum showed a positive reaction in the immunoblot with all CMS cytoplasms expressing the 16 kDa protein. Investigations of the mitochondrial DNA demonstrated that all ten CMS cytoplasms which express the 16 kDa protein have the same organization at the atpA locus. OrfH522 is located in the 3′-flanking region of the atpA gene. Transcript analyses using atpA and orfH522 as probes gave the same transcript pattern for the investigated CMS cytoplasms, just as for PET1. The MAX1 cytoplasm has an orfH522-related sequence but does not synthesize the 16 kDa protein. Using the sodium carbonate treatment the 16 kDa protein proved to be membrane-bound. Computer analyses predict that the hydrophobic N-terminal region of ORFH522 may form a transmembrane helix functioning as membrane anchor.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00049329
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  • 5
    Electronic Resource
    Electronic Resource
    A mitochondrial 16 kDa protein is associated with cytoplasmic male sterility in sunflower (1991)
    Springer
    Plant molecular biology 17 (1991), S. 29-36 
    Details
    ISSN: 1573-5028
    Keywords: CMS ; in organello translation ; mitochondria ; RNA ; Helianthus annuus
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Cytoplasmic male-sterile lines CMS89 and CMSBaso of sunflower (Helianthus annuus) differ from the fertile lines HA89 and Baso in a mitochondrial DNA sequence in the vicinity of theatpA gene. In addition, the transcriptional pattern of theatpA gene is changed in male-sterile lines compared to fertile ones. Besides one main transcript in the fertile lines, the male-sterile lines additionally show larger transcripts. Investigation of Baso and CMSBaso revealed that the two fertility-restored lines of CMS89 have the same transcripts as CMSBaso or a combination of CMSBaso and CMS89. Comparing the mitochondrialin organello translation products we observed a unique 16 kDa protein, which is expressed in male-sterile lines carrying theH. petiolaris cytoplasm but is not detectable in fertile lines withH. annuus cytoplasm. The 16 kDa protein can also be observed in restored lines but not inH. petiolaris. As the expression of the 16 kDa polypeptide seems to be linked to the interspecific cross betweenH. petiolaris andH. annuus it may play a role in CMS. By different criteria such as molecular mass, isoelectric point and peptide fingerprinting the α subunit of the F1-ATPase of male-sterile and fertile lines is very similar if not identical.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00036803
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  • 6
    Electronic Resource
    Electronic Resource
    Cytoplasmic male sterility in sunflower is correlated with the co-transcription of a new open reading frame with the atpA gene (1991)
    Springer
    Molecular genetics and genomics 227 (1991), S. 369-376 
    Details
    ISSN: 1617-4623
    Keywords: Cytoplasmic male sterility ; Sunflower ; atpA ; orfH522 ; Co-transcription
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The organization and expression of the mitochondrial (mt) genome of fertile, male-sterile and restored lines of Helianthus annuus and of H. petiolaris were compared to identify alterations which might lead to cytoplasmic male sterility (CMS). The mtDNAs of fertile and male-sterile lines differ by an 11 kb inversion and a 5 kb insertion. The rearrangements seem to be the result of recombination events within an inverted repeat of 261 bp. Detectable alterations in the transcript pattern of the rearranged mtDNA regions are restricted to the atpA locus. The male-sterile line CMSBaso shows three additional transcripts of the atpA locus of about 2500, 1200 and 250 nucleotides which are not detectable in Baso. However, the coding sequences of the atpA gene are entirely identical in the fertile line Baso and the male-sterile line CMSBaso. But a new open reading frame (orfH522) of 522 nucleotides is co-transcribed with the atpA gene as an additional larger transcript of about 2500 nucleotides in CMSBaso. orfH522 is also included in a second additional transcript of about 1200 nucleotides. The predicted translation product of orfH522 might play a role in CMS in sunflower.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00273925
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  • 7
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    AFLP assessment of the genetic diversity of Calotropis procera (Apocynaceae) in the West Africa region (Benin) (2014)
    Springer
    Details
    Publication Date: 2014-12-24
    Print ISSN: 0925-9864
    Electronic ISSN: 1573-5109
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Published by Springer
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  • 8
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    Genotyping of red and white fruited strawberry (Fragaria L.) accessions and hybrids based on microsatellite markers and on the genetic diversity in the allergen genes fra a 1 and fra a 3 (2015)
    Springer
    Details
    Publication Date: 2015-09-15
    Print ISSN: 0925-9864
    Electronic ISSN: 1573-5109
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Published by Springer
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  • 9
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    The Investigation of Perennial Sunflower Species (Helianthus L.) Mitochondrial Genomes (2020)
    Molecular Diversity Preservation International
    Details
    Publication Date: 2020-08-24
    Description: The genus Helianthus is a diverse taxonomic group with approximately 50 species. Most sunflower genomic investigations are devoted to economically valuable species, e.g., H. annuus, while other Helianthus species, especially perennial, are predominantly a blind spot. In the current study, we have assembled the complete mitogenomes of two perennial species: H. grosseserratus (273,543 bp) and H. strumosus (281,055 bp). We analyzed their sequences and gene profiles in comparison to the available complete mitogenomes of H. annuus. Except for sdh4 and trnA-UGC, both perennial sunflower species had the same gene content and almost identical protein-coding sequences when compared with each other and with annual sunflowers (H. annuus). Common mitochondrial open reading frames (ORFs) (orf117, orf139, and orf334) in sunflowers and unique ORFs for H. grosseserratus (orf633) and H. strumosus (orf126, orf184, orf207) were identified. The maintenance of plastid-derived coding sequences in the mitogenomes of both annual and perennial sunflowers and the low frequency of nonsynonymous mutations point at an extremely low variability of mitochondrial DNA (mtDNA) coding sequences in the Helianthus genus.
    Electronic ISSN: 2073-4425
    Topics: Biology
    Published by Molecular Diversity Preservation International
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  • 10
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    Organization Features of the Mitochondrial Genome of Sunflower (Helianthus annuus L.) with ANN2-Type Male-Sterile Cytoplasm (2019)
    Molecular Diversity Preservation International
    Details
    Publication Date: 2019-10-23
    Description: This study provides insights into the flexibility of the mitochondrial genome in sunflower (Helianthus annuus L.) as well as into the causes of ANN2-type cytoplasmic male sterility (CMS). De novo assembly of the mitochondrial genome of male-sterile HA89(ANN2) sunflower line was performed using high-throughput sequencing technologies. Analysis of CMS ANN2 mitochondrial DNA sequence revealed the following reorganization events: twelve rearrangements, seven insertions, and nine deletions. Comparisons of coding sequences from the male-sterile line with the male-fertile line identified a deletion of orf777 and seven new transcriptionally active open reading frames (ORFs): orf324, orf327, orf345, orf558, orf891, orf933, orf1197. Three of these ORFs represent chimeric genes involving atp6 (orf1197), cox2 (orf558), and nad6 (orf891). In addition, orf558, orf891, orf1197, as well as orf933, encode proteins containing membrane domain(s), making them the most likely candidate genes for CMS development in ANN2. Although the investigated CMS phenotype may be caused by simultaneous action of several candidate genes, we assume that orf1197 plays a major role in developing male sterility in ANN2. Comparative analysis of mitogenome organization in sunflower lines representing different CMS sources also allowed identification of reorganization hot spots in the mitochondrial genome of sunflower.
    Electronic ISSN: 2223-7747
    Topics: Biology
    Published by Molecular Diversity Preservation International
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