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  • 1
    Series available for loan
    Series available for loan
    Hannover : Schweizerbart
    Associated volumes
    Call number: SR 99.0054(2)
    In: Geologisches Jahrbuch
    Type of Medium: Series available for loan
    Pages: 63 S. + 2 Kt.-Beil., 1 Beil.
    Series Statement: Geologisches Jahrbuch : Reihe B 2
    Language: German
    Location: Lower compact magazine
    Branch Library: GFZ Library
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Development genes and evolution 189 (1980), S. 215-219 
    ISSN: 1432-041X
    Keywords: Mouse eggs ; Microsurgical enucleation ; Maternal mRNA ; Protein synthesis ; 2-Dimensional polyacrylamide gel electrophoresis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Fertilized and unfertilized C57BL/6J eggs were microsurgically enucleated and then analyzed for their capacity to synthesize proteins using 2-dimensional polyacrylamide gel electrophoresis. In both types of enucleated eggs (cytoplasts), protein synthesis continued and was still detected up to three days in culture. Shortly after enucleation, the pattern of polypeptides remained similar to the respective non-operated control eggs but it later became gradually reduced in intensity and complexity. After two days of culture the appearance of some new proteins typical for 2-cell embryos was observed in enucleated fertilized eggs only. Our findings suggest that maternal mRNA stored during oogenesis is utilized during the preimplantation period.
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 1432-041X
    Keywords: Neural induction ; Protein factors ; Xenopus laevis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary High (Mr ≈ 90–110 kDa) and low (Mr ≈ 15–30 kDa) molecular weight forms of neural-inducing factors have been found in the supernatant of Xenopus gastrula homogenate. The factors, which are protein in nature, have been partially purified by size exclusion high-performance liquid chromatography (HPLC) and sodium dodecyl sulphate (SDS)-polyacrylamide gel electrophoresis. The factor of smaller size, which could be derived from a precursor, is associated with other proteins in a larger complex. The neural-inducing factors are not irreversibly inactivated after chemical deglycosylation with trifluoromethansulfonic acid. The neural-inducing protein which is found in ribonucleoprotein (RNP)-particles was partially purified by hydrophobic chromatography. Possible relationships of the factors in different subcellular fractions and their physiological significance are discussed.
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    Springer
    Development genes and evolution 192 (1983), S. 45-47 
    ISSN: 1432-041X
    Keywords: Neuralizing factor ; Transmission ; Mesoderm ; Extracellular material ; Triturus embryo
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The neural plates of very early neurula stages of Triturus alpestris were removed, the material which is released from the extracellular space between mesoderm and neural plate to the medium in which the embryos were dissected was isolated and extracted with phenol. The protein isolated from the phenol layer showed neural inducting activity. Proteoglycans isolated from the aqueous layer did not show such inducing activity. These results together with previously published experiments (Wilhelm Roux's Arch 184: 285–299) suggest that a neuralizing factor which is released from the mesoderm acts on the inner surface of the overlying dorsal ectoderm.
    Type of Medium: Electronic Resource
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  • 5
    ISSN: 1432-041X
    Keywords: Mesoderm induction ; Xenopus XTC factor ; Chicken vegetalizing factor
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary We have compared the chemical properties and biological activities of the mesoderm-inducing factor that is secreted by the Xenopus XTC cell line with the vegetalizing factor from chicken embryos. The inducing activity of the factors was tested in different concentrations on totipotent ectoderm either by implantation into early gastrulae of Triturm alpestris or by application of solutions to isolated ectoderm of early gastrulae of Xenopus laevis. Both factors have similar properties. They are not irreversibly inactivated after treatment with 6 M urea or with phenol at 60° C. Reduction with thioglycolic acid inactivates the factors completely. The inducing activity of XTC-conditioned medium decreases only slightly after treatment with 50% formic acid. The apparent molecular mass and the isoelectric point of the factors are similar. The XTC factor was partially purified by size-exclusion and reversed-phase high-pressure liquid chromatography and by isoelectric focusing. The possible relationship of these factors to transforming growth factor β is discussed.
    Type of Medium: Electronic Resource
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  • 6
    Electronic Resource
    Electronic Resource
    Springer
    International journal of earth sciences 70 (1981), S. 637-647 
    ISSN: 1437-3262
    Source: Springer Online Journal Archives 1860-2000
    Topics: Geosciences
    Description / Table of Contents: Abstract In the Antarctic summer of 1979/80 the German Antarctic North Victoria Land Expedition (Ganovex 1979) carried out geoscientific investigations in the northern part of Victoria Land.Landsat satellite images from a section of the northeastern corner of Victoria Land, west of the Ross Sea, are described. These photos represent the first digital image processing for this area. Their value can be discerned already, especially in terms of our knowledge on the glaciology, geomorphology, and geology of this region.
    Abstract: Résumé Au cours de l'été antarctique 1979/1980, l'Expédition Allemande Antarctique de la Terre Victoria (Ganovex 1979) a effectué des recherches géoscientifiques sur la partie nord de la Terre Victoria. Nous présentons ici des images du satelliteLandsat de la partie nord-est de la Terre Victoria à l'ouest de la Mer de Ross. Ces images représentent le premier essai d'un traitement digital par images sur cette région. La valeur de ces essais est déja evidente en glaciologie, géomorphologie et géologie.
    Notes: Zusammenfassung Im antarktischen Sommer 1979/80 führte die Deutsche Antarktis-Expedition Nord-Victorialand (Ganovex 1979) geowissenschaftliche Untersuchungen im nördlichen Victorialand durch. Von einem Teilgebiet an der Nordostecke von Victorialand, westlich des Ross-Meeres, werden Bilder des SatellitenLandsat vorgestellt. Diese Aufnahmen sind die ersten Versuche einer digitalen Bildverarbeitung für dieses Gebiet. Sie lassen aber schon den Wert besonders für die Glaziologie, Geomorphologie und Geologie erkennen.
    Type of Medium: Electronic Resource
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  • 7
    Electronic Resource
    Electronic Resource
    [s.l.] : Nature Publishing Group
    Nature 365 (1993), S. 806-809 
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] The isotopic compositions of interstellar graphite grains in meteorites provide a record of the stellar environments in which they formed. The grains have 12C/13C ratios that range far from the terrestrial value, as well as large variations in ...
    Type of Medium: Electronic Resource
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  • 8
    Electronic Resource
    Electronic Resource
    [s.l.] : Nature Publishing Group
    Nature 339 (1989), S. 351-354 
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] Interstellar silicon carbide grains in meteorites provide a novel means for studying the carbon-star population of about 5 × 109 years ago. Their 12C/13C ratios differ greatly from the solar value but resemble those of present-day carbon stars, ...
    Type of Medium: Electronic Resource
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  • 9
    Electronic Resource
    Electronic Resource
    Springer
    Molecular genetics and genomics 180 (1980), S. 547-552 
    ISSN: 1617-4623
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary In preimplantation stages of normal and spontaneously activated parthenogenetic embryos of the LT/Sv mouse strain, protein synthesis was analyzed by using two-dimensional polyacrylamide gel electrophoresis. Fertilization and parthenogenetic activation cause similar changes of polypeptide synthesis when compared with those of unfertilized eggs. The overt developmental delay of early parthenotes, which is probably due to an initial retarded activation in comparison with normal fertilization, is documented molecularly by a similar delay in their protein synthesis pattern. These differences are clearly visible at the two-cell stage but gradually disappear during further cleavage. The basic protein patterns of normal and parthenogenetic embryos are remarkably similar up to the blastocyst stage. However, quantitative differences occur in all preimplantation embryos analyzed and become more distinct at the blastocyst stage. In addition, only minor qualitative changes appear during late preimplantation. These alterations in protein synthesis may reflect at the molecular level early events in abnormal development of parthenotes. Our biochemical results are discussed in context with biological experiments rescuing parthenogenetic LT/ Sv embryos by chimera formation.
    Type of Medium: Electronic Resource
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  • 10
    ISSN: 1617-4623
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary In order to compare paternal and maternal gene activity at the protein synthesis level during early development, androgenetic and gynogenetic mouse embryos were experimentally produced by microsurgically removing either the female or the male pronucleus from fertilized mouse eggs. The resulting haploid eggs were diploidized in a medium containing cytochalasin B and then cultured under normal conditions to the blastocyst stage. Protein synthesis was analyzed at different stages of preimplantation development using 2-dimensional polyacrylamide gel electrophoresis. Both types of uniparental embryos synthesized a similar set of proteins independent of whether the paternal or the maternal genome was present. The isodiploid embryos expressed a protein pattern that corresponded remarkably to normal embryos at the subsequent cleavage stage. This temporal change is probably due to the fact that the operated haploid eggs were kept overnight in cytochalasin B in order to allow chromosomal replication to occur without cell division, and the resulting eggs therefore corresponded to normal 2-cell embryos with respect to karyokinesis but differed as far as cytokinesis was concerned. Several 2-cell specific proteins appeared in these isodiploid eggs and, similarly, following their first cleavage some 4-cell specific proteins were detected in 2-cell androgenetic and gynogenetic embryos. The discordance between nuclear and cellular division, which was retained through the 4-cell stage, however disappeared during subsequent cleavage divisions. At the blastocyst stage, both kinds of uniparental embryos showed a similar protein pattern compared to normal embryos. Our data suggest that some stage-specific proteins are synthesized during preimplantation development and correspond to nuclear rather than cellular divisions.
    Type of Medium: Electronic Resource
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