ISSN:
1618-2650
Source:
Springer Online Journal Archives 1860-2000
Topics:
Chemistry and Pharmacology
Notes:
Conclusions During HPLC (reversed phase), tritiated steroids elute earlier than the unlabeled and the [4-14C]-labeled analogues. On formamide impregnated paper [4-14C]-labeled steroids move faster than the tritiated analogues. Thus, in both systems the 3H-labeled compounds behave as if they were more polar [1]. In the case of sixfold labeled estradiol the highly efficient resolution of the paperchromatographic and HPLC-systems (Fig. 1 A) may be applied to obtain carrier-free steroids with high specific activities. On the other hand the analytical application of these systems may lead to great systematic errors if the isotope effects of tritiated steroids are not taken into consideration.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00481795
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