ISSN:
1432-0878
Keywords:
Cerebrospinal fluid
;
Regeneration
;
Ependymins
;
Extracellular matrix
;
Collagen
;
Meninges
;
Immuno-electron microscopy
;
Oncorhynchus mykiss (Teleostei)
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Medicine
Notes:
Abstract In the rainbow trout, ependymins represent the predominant protein constituents of the cerebrospinal fluid (CSF) and perimeningeal fluid (PMF). Synthesis of these glycoproteins occurs exclusively in the endomeninx. Generally, ependymins share characteristics with proteins mediating cell-contact phenomena. Here, we show that the endomeninx of the rainbow trout is composed of three different layers, viz. an outer layer, an arachnoid-like intermediate barrier layer and an inner layer. This structure is in agreement with a meningeal barrier concept separating the PMF from the CSF. Furthermore, by immuno-electron microscopy, we have localized the majority of intracellular ependymins to the rough endoplasmic reticulum of fibroblast-like cells of the inner layer and to cells to the intermediate barrier layer. This pattern is compatible with the observed distribution of ependymins in both the PMF and CSF. In addition to their intracellular localization, an extracellular association of ependymins with bundles of collagen fibrils is demonstrated; this is particularly pronounced around all blood vessels of the brain.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00333696
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