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  • 1
    Electronic Resource
    Electronic Resource
    Amsterdam : Elsevier
    Agricultural Meteorology 18 (1977), S. 401-408 
    ISSN: 0002-1571
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Geography , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Physics
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    [s.l.] : Nature Publishing Group
    Nature 200 (1963), S. 1009-1009 
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] This work of Sutcliffe's could have great theoretical importance, and we have accordingly included chloramphenicol in studies of calcium uptake by isolated mitochondria from etiolated maize shoots. The experiments were patterned somewhat after those of Brierley et al.5 and Vasington and Murphy6. ...
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  • 3
    Electronic Resource
    Electronic Resource
    [s.l.] : Nature Publishing Group
    Nature 209 (1966), S. 425-426 
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] It is not known whether ion transport across other plant cell membranes, such as the plasmalemma or tonoplast, is similar to ion transport across mitochondrial membranes. However, it seems possible that a similar ATP-driven system with the formation of a high-energy intermediate or other ...
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  • 4
    Electronic Resource
    Electronic Resource
    [s.l.] : Nature Publishing Group
    Nature 215 (1967), S. 970-972 
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] The effect of azide on the partial reactions of phosphorylation, that is, the ATPase, the ATP?Pi and ATP?ADP exchange reactions, appear to be similar to that of oligomycin7?10. Wadkins and Lehninger9 suggested that both azide and oligomycin function by combining with a non-phosphorylated ...
    Type of Medium: Electronic Resource
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  • 5
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Weed research 16 (1976), S. 0 
    ISSN: 1365-3180
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: The absorption and loss of four chloro-s-triazines was investigated in excised roots of four Setaria taxa. Different taxa absorbed the various triazines at different rates. In general, triazine absorption was greater at 2°C than at 22.5°C, and absorption rates were linear functions of external concentrations.Efflux studies showed marked differences in the rate of loss of 14C-atrazine, 14C-simazine, and 14C-propazine from root sections of robust white foxtail (Setaria viridis var. robustaalba Schreiber). The roots lost 14C-atrazine very quickly, and the loss was similar in either water or 12C-atrazine. Atrazine appears to be restricted to the apoplast of the root. 14C-atrazine was lost more rapidly than either 14C-simazine or 14C-propazine to water or to solutions containing the unlabelled herbicide. Efflux of 14C-simazine was greater than that of 14C-propazine to solutions of CaCl2. From the pattern of efflux, it was concluded that 14C-simazine and 14C-propazine accumulated in the root symplast. Furthermore, the decreases in chloro-s-triazine absorption in the presence of metabolic inhibitors (dinitrophenol, sodium arsenite) may suggest that 14C-simazine and 14C-propazine entered the symplast by an energy-dependent process.
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  • 6
    ISSN: 0308-521X
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Type of Medium: Electronic Resource
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  • 7
    Electronic Resource
    Electronic Resource
    [s.l.] : Nature Publishing Company
    Nature biotechnology 4 (1986), S. 219-223 
    ISSN: 1546-1696
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: [Auszug] Genetic engineering of corn, and other cereals, is currently limited because of the difficulty of regenerating plants via cell culture. Here we report that commercially important inbred lines and hybrids from several different maize groups are capable of being regenerated via somatic embryos. The ...
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  • 8
    Electronic Resource
    Electronic Resource
    [s.l.] : Nature Publishing Group
    Nature 203 (1964), S. 258-261 
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] IN the course of investigating the accumulation of calcium, by maize mitochondria, it was observed that chloramphenicol in the concentrations used to inhibit ion accumulation by plants acted as an uncoupling agent1. Superficially, the compound seemed to act in the same fashion as the classic ...
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  • 9
    Electronic Resource
    Electronic Resource
    Springer
    Planta 172 (1987), S. 245-251 
    ISSN: 1432-2048
    Keywords: Embryonic callus ; Protoplast ; Zea (embryogenic callus) ; Transformation (protoplasts)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Maize (Zea mays L.) protoplasts have been obtained which divide rapidly and produce a callus that differentiates to form somatic embryos. The somatic embryos can be induced to form roots and small leaf-like structures. The genotype was the hybrid A188xBlack Mexican Sweet. Protoplasts were prepared from an embryogenic suspension culture derived from a Type II callus which had been selected from Type I callus produced by immature zygotic embryos. The basal medium for the suspension culture was N6 (C.C. Chu et al., 1975, Scientia Sinica 18, 659–668). The 2,4-dichlorophenoxyacetic acid concentration of the suspension culture was critical for subsequent protoplast growth and was optimal at 4.0 mg.l. Protoplasts had to be cultured in a low-osmoticum medium (0.3 M mannitol) for subsequent cell divisions to occur. The protoplasts have been transformed transiently with the gene chloramphenicol acetyltransferase (CAT) containing the 35S promoter obtained from cauliflower mosaic virus (CaMV-35S).
    Type of Medium: Electronic Resource
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  • 10
    Electronic Resource
    Electronic Resource
    Springer
    Planta 175 (1988), S. 280-289 
    ISSN: 1432-2048
    Keywords: Daucus ; Ionic current ; pH gradient extracellular ; Somatic embryo ; Vibrating probe
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A vibrating probe was used to measure extracellular electrical currents around developing somatic embryos in two lines (RCC27, RCC48) of cultured cells of Daucus carota L. at the heart and torpedo stages. At pH 5.5, an inward current of 1.2±0.1 μA·cm-2 (n=23) was detected at the cotyledon, and an outward current of 1.0±0.1 μA·cm-2 (n=22) was found at the radicle in torpedostage embryos from the RCC27 line. At a pH of 5.75 the currents increased by 0.2–0.3 μA·cm-2 (n=60–62). In a few cases an additional small inward current was detected at the tip of the radicle in toepedo-stage embryos from RCC27 line. Such an inward current at the radicle seemed to appear earlier, some time after the heart stage, in embryos from the RCC48 line. Both extracellular pH measurements (using microelectrodes filled with ion-sensitive resin) and ion-substitution studies were carried out in order to ascertain the ionic composition of the currents in torpedo-stage embryos from the RCC27 line. Regions adjacent to the cotyledon and radicle, at the points of current entry and exit, were found to be more acidic by 0.02±0.01 (n=14) and 0.07±0.01 (n=12) pH units, respectively, than the bulk medium. Removal of K+ from the medium reversibly reduced the currents to about 25% of their original value at both cotyledon and radicle. Deletion of Cl- decreased the currents slightly. Removal of Ca2+ resulted in a rapid doubling of currents. Addition of either N,N′-dicyclohexylcarbodiimide or tetraethyl ammonium chloride substantially reduced overall currents, and their removal resulted in partial recovery of the currents. It is suggested that the inward current at the cotyledon is comprised largely of K+ influx and the outward current at the radicle is mainly the result of active H+ efflux.
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