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  • 1
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    A gel-halo sample pellet: The best of both worlds? Stubby zones without a constricted sample gate (1985)
    In:  Other Sources
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    Publication Date: 2019-01-25
    Description: This reduces cell quantity, in the same way as does a narrower bore sample gate. The cells are confined to the center of the gate, inside a halo of electrolyte (D-1 buffer, here) immobilized by gel, which halo mechanically retains the ice pellet in position prior to thaw. The gel halo can be retained by the same gentle ridge as is contemplated for the ice pellet, and the ice pellet can be likewise retained within the gel halo.
    Keywords: INORGANIC AND PHYSICAL CHEMISTRY
    Type: Kidney Cell Electrophoresis; 3 p
    Format: text
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    NASA TECHNICAL REPORTS
  • 2
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    Role of gravity in preparative electrophoresis (1974)
    In:  Other Sources
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    Publication Date: 2019-01-25
    Description: Electrophoresis has contributed significantly to the methodology of biological sciences, and shows the potential for large scale fractionation of a wide range of medically important substances, including living cells. Gravity plays an important role in the electrophoretic process, and hence the importance of the NASA effort to develop a zero-gravity separation facility as part of its shuttle program. The current state of art in electrophoresis is reviewed with particular emphasis on the role of gravity and the possible use of istachophoresis. This technique utilizes a discontinuous buffer system, and appears to be the only high resolution electrophoretic technique currently available for separation of living cells.
    Keywords: BIOSCIENCES
    Type: Proc. of the 3d Space Processing Symp. on Skylab Results, Vol. 2; p 729-253
    Format: text
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    NASA TECHNICAL REPORTS
  • 3
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    Transphoresis and isotachophoresis as preparative techniques with reference to zero-gravity (1974)
    In:  Other Sources
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    Publication Date: 2019-07-13
    Description: Transphoresis and isotachophoresis are high resolution electrophoretic separation methods for biological materials. They benefit substantially from a weightless environment. Low power consumption and apparatus payload per unit product yield reflect their high electrophoretic efficiency. Properties unique to these systems, including their imposition of self-stabilizing geometry, suggest novel apparatus automatically controllable by largely electrical means with few or no moving parts. Configurations allowing continuous and semicontinuous operation, and further improvement of efficiency, are discussed.
    Keywords: BIOSCIENCES
    Type: AIAA PAPER 74-664 , Thermophysics and Heat Transfer Conference; Jul 15, 1974 - Jul 17, 1974; Boston, MA
    Format: text
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    NASA TECHNICAL REPORTS
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