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  • 1
    Electronic Resource
    Electronic Resource
    The capsid polypeptides of the yeast viruses
    Amsterdam : Elsevier
    Biochemical and Biophysical Research Communications 121 (1984), S. 619-625 
    Details
    ISSN: 0006-291X
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology , Chemistry and Pharmacology , Physics
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1016/0006-291X(84)90227-4
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  • 2
    Electronic Resource
    Electronic Resource
    Experimental cross section for individual vibrational quantum transitions in low energy central collisions of Li^+ with H"2
    Amsterdam : Elsevier
    Chemical Physics Letters 6 (1970), S. 304-306 
    Details
    ISSN: 0009-2614
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Chemistry and Pharmacology , Physics
    Type of Medium: Electronic Resource
    URL: http://linkinghub.elsevier.com/retrieve/pii/0009-2614(70)85081-3
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  • 3
    Electronic Resource
    Electronic Resource
    Zur umsetzung von diphenylcarbenpentacarbonylwolfram(0) mit trimethylphosphin
    Amsterdam : Elsevier
    Journal of Organometallic Chemistry 86 (1975), S. C10-C12 
    Details
    ISSN: 0022-328X
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://linkinghub.elsevier.com/retrieve/pii/S0022-328X(00)88522-8
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  • 4
    Electronic Resource
    Electronic Resource
    Ubergangsmetall-carben-komplexe
    Amsterdam : Elsevier
    Journal of Organometallic Chemistry 112 (1976), S. C59-C62 
    Details
    ISSN: 0022-328X
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://linkinghub.elsevier.com/retrieve/pii/S0022-328X(00)87124-7
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  • 5
    Electronic Resource
    Electronic Resource
    Comparative investigations on the metabolism of formaldehyde in the presence of ribose-5-phosphate in cell-free extracts of yeasts grown on methanol
    Amsterdam : Elsevier
    FEBS Letters 38 (1974), S. 274-276 
    Details
    ISSN: 0014-5793
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology , Chemistry and Pharmacology , Physics
    Type of Medium: Electronic Resource
    URL: http://linkinghub.elsevier.com/retrieve/pii/0014-5793(74)80071-2
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  • 6
    Electronic Resource
    Electronic Resource
    Controlled preparation of protoplasts ofKloeckera sp. 2201 grown on methanol (1977)
    Springer
    Applied microbiology and biotechnology 4 (1977), S. 21-27 
    Details
    ISSN: 1432-0614
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary Two methods for the controlled formation of protoplasts of the yeastKloeckera sp. 2201 grown on methanol as sole carbon source are presented. For the lysis of yeast cell walls, an extracellular enzyme, produced byArtbrobacter luteus and called Zymolyase-5000, is used. The carbon source of the growth medium greatly influences the susceptibility of the yeast cell wall against the attack of this enzyme. Glucose-grown cells can easily be converted into protoplasts without preceding incubation with β-mercaptoethanol, whereas cells grown on methanol require preincubation. A 20–30% decrease of O.D.650 indicates complete formation of protoplasts. A further decrease of O.D. is the result of partial bursting of the protoplasts. The formation of protoplasts in isotonic media starts with the formation of holes in the yeast cell wall. This enzymatic perforation is then followed by the release of the protoplasts. The type of hypertonic medium and its concentration have a great influence on the process of protoplast formation. The choice of a particular medium is a compromise between quantitative formation of protoplasts and sufficient stabilization of these.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01390666
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  • 7
    Electronic Resource
    Electronic Resource
    Continuous culture of Candida boidinii variant 60 growing on methanol (1978)
    Springer
    Applied microbiology and biotechnology 6 (1978), S. 127-132 
    Details
    ISSN: 1432-0614
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary A new variant, Candida boidinii variant 60, which is less sensitive to methanol and formaldehyde shocks was grown in continuous cultures with methanol as sole carbon source. The substrate concentration in the feeding medium was either 1% methanol or 3% methanol. Biomass production, methanol consumption, the formation of formaldehyde and gas exchange were measured at different dilution rates. With low methanol feeding (10 g/l) maximal productivity of 0.44 g biomass/l·h is obtained at a dilution rate of 0.14 h−1. Maximal specific growth rate is 0.18 h−1. A yield of 0.32 g biomass/g methanol was obtained and the respiration quotient was determined as 0.55. Independently of initial substrate concentration, biomass decreases if methanol and formaldehyde are accumulating in the culture broth. In the culture with high methanol feeding (30 g/l) cell concentratioon increases up to 9 g/l at D=0.04 h−1. At higher dilution rates methanol and form-aldehyde appear in the medium. Formaldehyde is then preferably oxidized without energy advantages for the cells. It seems that this enables the cells to overcome toxic effects caused by methanol and formaldehyde.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00504424
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  • 8
    Electronic Resource
    Electronic Resource
    Metabolic changes during substrate shifts in continuous cultures of the yeast Candida boidinii variant 60 (1978)
    Springer
    Applied microbiology and biotechnology 6 (1978), S. 133-144 
    Details
    ISSN: 1432-0614
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary Substrate shift experiments in chemostat cultures with either methanol or glucose as carbon source were performed with the yeast Candida boidinii variant 60. At low dilution rates of 0.064 h−1 the culture may be easily shifted from methanol to glucose medium and back again to methanol. From these experiments it can be seen that glucose does not give rise to any catabolite inhibition of alcohol oxidase. Alcohol oxidase and formaldehyde dehydrogenase seem to be regulated by a repression-derepression mechanism, as small basal activities of both these enzymes can still be measured during growth on glucose. On the other hand, formate dehydrogenase activity is completely absent in the presence of glucose. This kind of regulation seems to favor the smooth switch from growth on glucose to methanol metabolism. With methanol or glucose, growth yields (YS) of 0.3 and 0.35, respectively may be obtained, and oxygen consumption (QO 2) is much higher in methanol cultures than in glucose-grown cells. Accordingly, the RQ values during growth on methanol decrease to about 0.5. Based on the yield coefficient of 0.3, it is possible to calculate that 38% of the methanol consumed must be incorporated into biomass, whereas 62% of the methanol is oxidized to CO2. The corresponding RQ of 0.56 could not be experimentally ascertained. The activities of three mitochondrial enzymes were found to be higher in methanol-grown cells than in cells from glucose cultures. The low activites of enzymes for the phosphogluconate route in methanol-grown cells indicates that a cyclic oxidation of formaldehyde via hexose phosphate to CO2 cannot be of great importance for methanol metabolism.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00504425
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  • 9
    Electronic Resource
    Electronic Resource
    Eene Statistiek van Brandverzekering. (Vervolg op een artikel van Juni 1900) (1911)
    Springer
    De economist 60 (1911), S. 210-222 
    Details
    ISSN: 1572-9982
    Source: Springer Online Journal Archives 1860-2000
    Topics: Economics
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02199053
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  • 10
    Electronic Resource
    Electronic Resource
    Regulation phenomena in methanol consuming yeasts: An experiment with model discrimination (1981)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 23 (1981), S. 499-521 
    Details
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Assimilation as well as dissimilation of methanol in yeasts takes place through its oxidative intermediate formaldehyde which is several times more toxic to the growth of microorganisms than methanol itself. Still, the role of formaldehyde, produced during methanol assimilation, upon growth of yeasts is not clear. In the present paper, an attempt has been made to throw some light upon this aspect. Starting with a basic frame work for methanol uptake by yeasts, several models were developed assuming different modes of regulation of key enzymes by methanol and/or formaldehyde. The main feature of the basic framework consists in consideration of two routes for oxidation of formaldehyde to CO2, one associated and the other not associated with production of energy. Further, the rate of energy production form the energy-associated oxidation of formaldehyde is assumed to be controlled by the rate of energy consumption by anabolic reactions. The models were discriminated by subjecting these to biological constraints. As a result, the successful model suggests that in spite of higher inherent toxicity of formaldehyde, methanol exerts the controlling influence upon growth under normal conditions.
    Additional Material: 9 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bit.260230304
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