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  • 1
    Electronic Resource
    Electronic Resource
    Genetics of the peroxidase isoenzymes in Petunia (1984)
    Springer
    Theoretical and applied genetics 68 (1984), S. 265-268 
    Details
    ISSN: 1432-2242
    Keywords: Petunia ; prxA alleles ; Specific peroxidase activities
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Antibodies were raised against the peroxidases encoded by the allele prxA1 to determine the specific activities of the peroxidases encoded by the alleles prxA1, prxA2, prxA3, and prxA5. The results from double diffusion experiments indicated that all peroxidases encoded by the four alleles are antigenically identical. By rocket immuno electrophoresis it was shown that the peroxidases encoded by the alleles prxA1, prxA2, prxA3, and prxA5 have different specific activities. The results presented are discussed in relation to differential expression of the alleles involved.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00266901
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  • 2
    Electronic Resource
    Electronic Resource
    Genetics of the peroxidase isoenzymes inPetunia (1985)
    Springer
    Theoretical and applied genetics 69 (1985), S. 349-351 
    Details
    ISSN: 1432-2242
    Keywords: Petunia ; Peroxidase isoenzymes ; Gene localization
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The structural geneprxD inPetunia codes for a slow moving anodic peroxidase whose activity is sensitive to high concentrations of hydrogen peroxide. The PRXd enzyme could be found in mature and old leaf and stem tissue of full-grown flowering plants. PRXd was found to be absent in tissues from flower corolla and root. The geneprxD is the fourth gene that codes for peroxidases in leaf and stem. Two mobility variants of the PRXd enzyme have been found among our inbred lines using starch gel system II electrophoresis. The geneprxD could be located on chromosome III by a four-point-cross involving the genesprxA, prxD, Mf1 andHt1. The order of the genes established is:Ht1 — Mf1 — prxD — prxA.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00570899
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  • 3
    Electronic Resource
    Electronic Resource
    Distribution of sequences related to the Bg  transposable element of maize in Zea and related genera (1996)
    Springer
    Theoretical and applied genetics 92 (1996), S. 696-701 
    Details
    ISSN: 1432-2242
    Keywords: Key words Bg transposable element ; Molecular similarity ; Maize ; Teosinte ; Southern analysis ; Zea
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  Thirty-four accessions from Zea and 10 accessions from related genera were assayed for the presence of Bg, a transposable element originally found in maize (Zea mays ssp. mays). Bg-like sequences, identified as hybridizing bands on Southern blots, were visualized in all Zea accessions and were present in approximately equal numbers in teosinte and maize. With the exception of Tripsacum dactyloides, all accessions from related genera failed to hybridize with the Bg probes, even at reduced stringency. A comparison of the restriction patterns of related inbred lines revealed numerous common hybridizing fragments. An index of molecular similarity (MS) was used to determine the degree of similarity between pairs of inbred lines. Computed MS values endorse an inbred relationship and are in good agreement with published results of cluster analysis on these inbred lines.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001220050181
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  • 4
    Electronic Resource
    Electronic Resource
    Distribution of sequences related to the Bg transposable element of maize in Zea and related genera (1996)
    Springer
    Theoretical and applied genetics 92 (1996), S. 696-701 
    Details
    ISSN: 1432-2242
    Keywords: Bg transposable element ; Molecular similarity ; Maize ; Teosinte ; Southern analysis ; Zea
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Thirty-four accessions from Zea and 10 accessions from related genera were assayed for the presence of Bg, a transposable element originally found in maize (Zea mays ssp. mays). Bg-like sequences, identified as hybridizing bands on Southern blots, were visualized in all Zea accessions and were present in approximately equal numbers in teosinte and maize. With the exception of Tripsacum dactyloides, all accessions from related genera failed to hybridize with the Bg probes, even at reduced stringency. A comparison of the restriction patterns of related inbred lines revealed numerous common hybridizing fragments. An index of molecular similarity (MS) was used to determine the degree of similarity between pairs of inbred lines. Computed MS values endorse an inbred relationship and are in good agreement with published results of cluster analysis on these inbred lines.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00226091
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  • 5
    Electronic Resource
    Electronic Resource
    Discrimination between cultivars of Vitis vinifera based on molecular variability concerning 5″ untranslated regions of the StSy-CHS genes (1997)
    Springer
    Theoretical and applied genetics 95 (1997), S. 375-383 
    Details
    ISSN: 1432-2242
    Keywords: Key words Cluster analysis ; Genetic variability ; Ligation-mediated PCR ; Stilbene synthase ; Vitis vinifera
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  The degree of polymorphism present in 5′ untranslated regions of stilbene synthase (StSy)-like loci was assessed. A ligation-mediated polymerase chain reaction (LM-PCR) cloning strategy was adopted to isolate sequences located immediately upstream of StSy coding regions. Among several clones, 13 randomly chosen fragments were analyzed at the sequence level. Four of the analyzed fragments appeared of particular interest. Two carried sequences reminiscent of micro-satellites, while the remaining fragments contained direct repeats. Oligonucleotides constructed against the specific DNA sequence of these clones disclosed a complex banding pattern when used in polymerase chain reaction (PCR)-analysis of 22 ancient varieties of grapevine. A total of 40 polymorphic bands could be identified and used to calculate coefficients of genetic similarity (GS) between varieties. GS values were used in cluster analysis to differentiate the 22 varieties. The data obtained are in good agreement with available information concerning the relationships between the varieties considered. This suggests the use of the method we have developed in fingerprinting studies of Vitis vinifera germ plasma.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001220050573
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  • 6
    Electronic Resource
    Electronic Resource
    A New Method for Rapid Extraction of High Quality RNA from Recalcitrant Tissues of Grapevine (1998)
    Springer
    Plant molecular biology reporter 16 (1998), S. 61-67 
    Details
    ISSN: 1572-9818
    Keywords: Vitis ; RNA extraction ; solid phase extraction
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A quick, inexpensive, and reliable protocol for the extraction of RNA from grapevine berry skins containing large quantities of polyphenols, procyanidins, and polysaccharides is described. The method involves an extraction step in the presence of ribonuclease inhibitors and compounds that compete with vacuolar contaminants for binding to RNA. After extraction with organic solvents, RNA is bound to a fibrous cellulose matrix and processed to eliminate the remaining contaminants and ribonucleases. Following this method, highly stable RNA, sufficiently pure for northern hybridizations and enzymatic processing, may be obtained from as little as 200 mg of starting amounts of fresh material and without multiple, time consuming precipitations or ultracentrifugation steps. This procedure may also prove useful for extracting RNA from recalcitrant tissues of other plant species. Abbreviations: ATA, aurintricarboxylic acid; CF11, cellulose fibrous medium (type 11); PVPP, polyvinylpolypyrrolidone; RT room temperature; VRC, vanadyl ribonucleoside complex.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1007433910213
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  • 7
    Electronic Resource
    Electronic Resource
    Identification and characterisation of an RPD3 homologue from maize (Zea mays L.) that is able to complement an rpd3 null mutant of Saccharomycescerevisiae (1998)
    Springer
    Molecular genetics and genomics 258 (1998), S. 288-296 
    Details
    ISSN: 1617-4623
    Keywords: Key wordsZea mays ; Saccharomyces cerevisiae ; Gene regulation ; Histone deacetylase
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract In mammals, yeast and Drosophila, the histone deacetylase RPD3 proteins can alter the expression of genes involved in fundamental biological processes by affecting the degree of acetylation of histones and changing chromatin structure. Here we report the isolation of a cDNA sequence encoding an RPD3 homologue from maize, which is able to complement the phenotype of an rpd3 null mutant of the yeast Saccharomyces cerevisiae. The expression of the corresponding gene(s) was assessed in different maize tissues. The number of homologous loci was estimated by Southern hybridisation to be in the range of two to three, and the chromosomal location of one of these loci was determined. Phylogenetic analysis and tests for relative divergence rates, using related RPD3 sequences from different species, were performed, and suggest that different polymorphic forms of RPD3-like proteins that evolve at distinct rates are present in the species considered.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004380050733
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  • 8
    Electronic Resource
    Electronic Resource
    Molecular analysis of theBg-rbg transposable element system ofZea mays L. (1991)
    Springer
    Molecular genetics and genomics 227 (1991), S. 91-96 
    Details
    ISSN: 1617-4623
    Keywords: Zea mays ; Transposable elements ; Opaque-2 ; Waxy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The two components of theBg-rbg transposable element system of maize have been cloned. TheBg element, isolated from the mutable allelewx-m32 :: Bg is inserted in the intron of theWaxy (Wx) gene between exons 12 and 13. The length of the element is of 4869 bp.Bg has 5 by terminal inverted repeats, and generates upon insertion an 8 by direct duplication of the target sequence. Both ends of theBg element contain a 76 by direct repeat adjacent to the terminal inverted repeats. The hexamer motif TATCGkC G is here repeated several times in direct or inverse orientation. Therbg element was isolated from the mutable alleleo2m(r) where it is located in the promoter region of theOpaque-2 (O2) gene.rbg is approximately 4.5 kb in length, has terminal inverted repeats identical to those of theBg element, and is also flanked by an 8 by direct duplication at the target site. LikeBg, rbg carries the 76 by direct repeats. Restriction enzyme analysis reveals that, compared toBg, the receptor element is distinguishable by small deletion and insertion events. Sequence data indicate that not more than 75% homology exists at the DNA level between therbg element and the autonomousBg element.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00260712
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  • 9
    Electronic Resource
    Electronic Resource
    The b-32 protein from maize endosperm, an albumin regulated by the O2 locus: Nucleic acid (cDNA) and amino acid sequences (1988)
    Springer
    Molecular genetics and genomics 212 (1988), S. 481-487 
    Details
    ISSN: 1617-4623
    Keywords: Zein regulation ; O2 ; O6 ; b-32 protein ; cDNA cloning
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The cDNA coding for the b-32 protein, an albumin expressed in maize endosperm cells under the control of the O2 and O6 loci, has been cloned and the complete amino acid sequence of the protein derived. A lambda gt11 cDNA library from mRNA of immature maize endosperm was screened for the expression of the b-32 protein using antibodies against the purified protein. One of the positive clones obtained was used to isolate a full-length cDNA clone. By Northern analysis, the size of the b-32 mRNA was estimated to be 1.2 kb. Hybrid-selected translation assays show that the message codes for a protein with an apparent molecular weight of 30–35 kDa. The nucleotide sequence shows that several internal repeats are present. The protein has a length of 303 amino acid residues (mol. wt. 32430 dalton) and its sequence shows the following features: no signal peptide is observable; it contains seven tryptophan residues, an amino acid absent in maize storage proteins; polar and hydrophobic residues are spread along the sequence; several pairs of basic residues are present in the N-terminal region; the secondary structure allows the prediction of two structural domains for the b-32 protein that would fold up giving rise to a globular shape. The cloning of this gene may help in understanding the role of the O2 and O6 loci in regulating the deposition of zein, the major storage protein of maize endosperm.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00330853
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  • 10
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    The sequence of the zein regulatory geneopaque-2 (O2) ofZea Mays (1989)
    Oxford University Press
    Details
    Publication Date: 1989-01-01
    Print ISSN: 0305-1048
    Electronic ISSN: 1362-4962
    Topics: Biology
    Published by Oxford University Press
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