DNA fingerprinting, cultivar identification
simple repetitive sequences
Springer Online Journal Archives 1860-2000
Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
Summary Soybean DNA fingerprints were analyzed by digoxigenin-labeled oligonucleotide probes complementary to simple repetitive sequences. The clearest and most polymorphic patterns were obtained with (AAT)6 as a probe, with which all 47 soybean cultivars tested could be distinguished. However, DNA fingerprints of individuals within cultivars showed the same pattern. Using (CT)8, (GAA)5 or (AAGG)4 as probes, clear polymorphic patterns among cultivars and accessions in the subgenus Soja (Glycine max and Glycine soja) were not observed, while quite different patterns were found in accessions in the subgenus Glycine. The results suggest that G. max and G. soja are closer in their genome structure. DNA fingerprints of reciprocal crosses between cultivars and accessions in the subgenus Soja were similar, and contained bands of both parents. In an F2 population from these crosses, such bands segregated in a Mendelian fashion.
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