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  • 1
    Electronic Resource
    Electronic Resource
    s.l. : American Chemical Society
    Analytical chemistry 49 (1977), S. 390-395 
    ISSN: 1520-6882
    Source: ACS Legacy Archives
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    s.l. : American Chemical Society
    Analytical chemistry 23 (1951), S. 670-673 
    ISSN: 1520-6882
    Source: ACS Legacy Archives
    Topics: Chemistry and Pharmacology
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  • 3
    Electronic Resource
    Electronic Resource
    s.l. : American Chemical Society
    Journal of the American Chemical Society 28 (1906), S. 1198-1201 
    ISSN: 1520-5126
    Source: ACS Legacy Archives
    Topics: Chemistry and Pharmacology
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  • 4
    Electronic Resource
    Electronic Resource
    Springer
    Marine biology 46 (1978), S. 101-107 
    ISSN: 1432-1793
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The anaerobic microbial uptake of alanine and aspartic acid was determined in 4 diverse salt-marsh soils (tall and short Spartina alterniflora, creek bank, and mud flat). Uptake in soil slurries was determined by the radioisotopic tracer technique at one substrate concentration (〈250 pmoles cm-3). Dissolved free alanine and aspartic acid concentrations in the interstitial nutrient pool ranged from approximately 1 to 500 pmoles cm-3. In the short S. alterniflora soil, maximum microbial uptake of alanine was found at a depth of 10 cm (8.32 pmoles cm-3 h-1); in the tall S. alterniflora soil maximum uptake was at 20 cm (23.4 pmoles cm-3 h-1). The utilization of aspartic acid appeared constant over the depth interval investigated (0 to 60 cm). The turnover times of alanine and aspartic acid in the tall and short S. alterniflora soils ranged from 5 to 25 h and 40 to 100 h, respectively. The percent of the labeled alanine and aspartic acid taken up that was mineralized by tall and short s. alterniflora microbenthos ranged from 20 to 50% and 5 to 20%, respectively.
    Type of Medium: Electronic Resource
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  • 5
    ISSN: 1432-1793
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Differential uptake of [3H]adenine and [3H]thymidine, and incorporation of tritium label into proteins, RNA and DNA as a function of depth, indicate that surface microplankton incorporated most of the assimilated radioisotopes into nucleic acids. Growth processes for deep-sea microplankton were shifted towards higher rates of [3H]-adenine incorporation into RNA compared to rates of thymidine incorporation into DNA. Deep-sea microplankton also diverted a larger portion (up to 80%) of the assimilated tritium into biosynthetic pathways for amino acid and eventual incorporation into proteins. These results imply that protein synthesis is vital for deep-sea microplankton where populations may be stressed by low levels of available nutrients. The rates at which microplankton incorporated [3H]adenine into RNA and DNA, and [3H]thymidine into DNA, suggest that oceanic microplankton are in a transient state of balanced growth, i.e. between two steady-state growth conditions, irrespective of potential growth rates. Our results support the hypothesis that oceanic microplankton are in various growth states.
    Type of Medium: Electronic Resource
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  • 6
    ISSN: 1432-1793
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract An investigation was undertaken to determine the distribution and activity of the heterotrophic microplankton associated with particles from a Georgia salt marshestuarine ecosystem to the western edge of the Sargasso Sea. Heterotrophic activity was determined by the uptake of 14C-glucose. More than 80% of the activity was associated with detritus greater than 3 μm in creek, river and coastal (within 4 km of shore) waters. In the Gulf Stream, approximately 80% of the heterotrophic activity was in the fraction less than 3 μm. In the estuary, total heterotrophic activity fluctuated with the tides; the greatest activity occurred near low ebb tide at all locations. The lowest activity was measured at slack low and high tides. In creek water most of the heterotrophic activity was associated with particles between 14 and 180 μm, whereas in coastal waters (less than 4 km from shore) most of the activity was in the fraction greater than 180 μm.
    Type of Medium: Electronic Resource
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  • 7
    Electronic Resource
    Electronic Resource
    Springer
    Journal of molecular evolution 13 (1979), S. 233-244 
    ISSN: 1432-1432
    Keywords: Tubulin:tyrosine ligase ; Microtubules ; Cytoskeleton ; Tubulin post-translational modification
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The post-translational addition of tyrosine toa-tubulin, catalyzed by tubulin:tyrosine ligase, has been previously reported in mammals and birds. The present study demonstrated that significant ligase activity was present in representative organisms from several other major vertebrate classes (chondrichthyes through reptiles) and that both substrate and enzyme from all vertebrates investigated were compatible with mammalian ligase and tubulin in the tyrosination reaction. None of the invertebrate tissues examined showed incorporation of tyrosine, phenylalanine or dihydroxyphenylalanine intoa tubulin under conditions allowing significant incorporation of these compounds in vertebrate supernatant samples. The failure of invertebrate tubulin to incorporate tyrosine in vitro did not appear to be due to saturation of the carboxyl terminal position with tyrosine or the presence of a soluble inhibitor of ligase activity. Although tubulin amino acid composition has been highly conserved throughout evolution, a major evolutionary divergence is described based upon biochemical differences whereby invertebrate tubulin cannot be tyrosinated or posttranslationally modified with phenylalanine or dihydroxyphenylalanine under conditions suitable for the incorporation of these compounds by vertebratea tubulin.
    Type of Medium: Electronic Resource
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  • 8
    Electronic Resource
    Electronic Resource
    Springer
    Experiments in fluids 10 (1990), S. 41-49 
    ISSN: 1432-1114
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Abstract The planar laser-induced fluorescence (PLIF) technique is attractive for instantaneous and non-intrusive imaging of species concentration in gaseous flows. This paper provides a framework for determining the experimental resolution in PLIF experiments and gives error estimates for concentration measurements in turbulent jet mixing experiments using biacetyl as the molecular tracer. The procedures to correct for experimental artifacts in the PLIF images are outlined. Images of the instantaneous, average, rms, and dissipation of concentration in a turbulent jet are presented.
    Type of Medium: Electronic Resource
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  • 9
    ISSN: 1432-0649
    Keywords: 07.20Ka ; 47.40.Ki ; 47.70.Fw
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract An instantaneous temperature imaging technique for chemically reacting, supersonic flows over bodies is described and demonstrated in a H2/O2/Ar shock tube flow (M=1.3, 0.7 atm, 1760K freestream). Based on a planar fluorescence measurement, the approach uses a two-line rotational population ratio to infer temperature. The measured 2-d temperature profiles qualitatively match the expected flowfield structure around the blunt body, and the temperature increase across the bow shock in a single-shot measurement agrees within 5–10% of the prediction of a 1-d shock analysis. The significant systematic error sources for the technique are detailed, and the random error effects associated with shot-noise-limited fluorescence images are statistically analyzed to identify transitions which minimize the temperature errors for instantaneous and average measurements. Even for average temperature measurements, the analysis predicts errors which can be as large as 5–10% when noisy fluorescence images are used in conjunction with low temperature sensitivity. In general, temperature errors can be reduced by increasing sensitivity, i.e., the energy separation of the two rotational levels, until the fluorescence shot-noise rises to a value of 30–50% within the temperature range of interest.
    Type of Medium: Electronic Resource
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  • 10
    Electronic Resource
    Electronic Resource
    College Park, Md. : American Institute of Physics (AIP)
    The Journal of Chemical Physics 87 (1987), S. 3016-3021 
    ISSN: 1089-7690
    Source: AIP Digital Archive
    Topics: Physics , Chemistry and Pharmacology
    Notes: The Raman spectra and phase diagram of fluorine were studied in a diamond-anvil cell up to 6 GPa over the temperature range 10–300 K. The sample slowly reacted with the diamond anvils to form CF4. The vibron frequencies in α-F2 and β-F2, as well as the lattice modes in α-F2, were determined as a function of pressure. No new phases were discovered. The α–β phase boundary can be fit with an equation of the Simon form: Pαβ (GPa)=−0.385(10) +4.80(9)×10−4 Tαβ1.75 (K). The melting curve was established to lie between limiting curves: Puppermelt (GPa)=−0.107+1.01×10−4T1.75melt (K) and Plowermelt (GPa)=−0.140+1.32×10−4 T1.75melt (K).
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