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  • 1
    Electronic Resource
    Electronic Resource
    [s.l.] : Nature Publishing Group
    Nature 198 (1963), S. 509-510 
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] When HeLa cells are infected with herpes virus the agent escapes by budding out through cellular mem branes and becomes enclosed by an outer covering from these as it passes through and matures5. It seemed possible, therefore, that if budding were to occur at an active plasma membrane having ATPase ...
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  • 2
    Electronic Resource
    Electronic Resource
    [s.l.] : Nature Publishing Group
    Nature 170 (1952), S. 1012-1014 
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] IN response to many requests for further details of a new method for the localization of hydrolytic enzymes in tissues using indoxyl derivatives1, it is felt that a further brief report is desirable before publication of a more extensive critical account. With derivatives of indoxyl itself, for ...
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  • 3
    Electronic Resource
    Electronic Resource
    [s.l.] : Nature Publishing Group
    Nature 169 (1952), S. 271-273 
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] THE past twelve years have seen considerable effort A directed to the development of methods for the visualization of the distribution of hydrolytic enzymes in animal tissues. Such methods have been based on the formation of coloured pigments or dyes : (a) after or (b) during the enzymic breakdown ...
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  • 4
    Electronic Resource
    Electronic Resource
    [s.l.] : Nature Publishing Group
    Nature 191 (1961), S. 832-832 
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] This was observed when the osmium tetroxide in Palade's veronal-acetate buffered fixative4 was replaced by sufficient formaldehyde to give a final concentration of 4 per cent. The composition of the mixture was 2-6 ml. of analytical reagent quality 36 per cent formaldehyde, 5 ml. each of Mil sodium ...
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  • 5
    Electronic Resource
    Electronic Resource
    [s.l.] : Nature Publishing Group
    Nature 223 (1969), S. 1367-1368 
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] Frozen sections (20 (jt,m) of cold aldehyde-fixed8 first molars of 3 day old rats were decalcified in acetate-sucrose solution6 for periods of 1 to 2 h before incubation in the Gomori acid phosphatase medium for 15-60 min. They were then postfixed in osmium tetroxide, dehydrated in graded alcohols ...
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  • 6
    Electronic Resource
    Electronic Resource
    [s.l.] : Nature Publishing Group
    Nature 159 (1947), S. 714-715 
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] G. L. KESTEVEN has stressed1 the need for a dynamic approach to the study of fish populations. It has been possible recently to develop a mathematical treatment having certain features in common with that of Baronov2, which bears this necessity in mind, and which ...
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  • 7
    Electronic Resource
    Electronic Resource
    [s.l.] : Nature Publishing Group
    Nature 187 (1960), S. 1050-1051 
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] Similar methods have now been used writh type 5 adenovirus. The virus was grown in HeLa cell cultures3, and particles were isolated from them by a nuorocarbon separation technique4.5 followed by high-speed centrifugation. Samples of the particle-containing zones of the resulting pellets were used ...
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  • 8
    Electronic Resource
    Electronic Resource
    [s.l.] : Nature Publishing Group
    Nature 252 (1974), S. 437-437 
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] SIR,?Wendy Barnaby's otherwise fair account of the Pacem in Maribus Convocation (Nature, October 11) gives the impression that I said the "non-conventional" living resources of the ocean?such as squids, krill, lantern-fishes?are found mainly in the area beyond the proposed Exclusive Economic Zone. ...
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  • 9
    Electronic Resource
    Electronic Resource
    Springer
    Bioscience reports 5 (1985), S. 159-166 
    ISSN: 1573-4935
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract The number of high affinity [3H]GDP binding sites in brown adipose tissue mitochondria is normal in obese ( f a / f a ) rats in contrast to the reduced number of low affinity GDP binding sites. Adrenalectomy corrected the loss of low affinity binding sites in fa/fa rats but had no effect on the number of high affinity sites in either lean or obese rats. Equilibrium dialysis was used to show the presence of both high and low affinity binding sites on the purified 32 kdalton protein.
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  • 10
    ISSN: 1573-6865
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Synopsis The activity and ultrastructural localization of alkaline phosphatase and esterase has been studied in normal rat intestine and after the increased crypt cell proliferation that occurs during recovery after 400 rad X-irradiation. Alkaline phosphatase activity is not present in crypt cells of normal intestine, but becomes apparent after the cell has migrated on to the villus. The enzyme is localized in the microvilli, along the lateral cell membranes and in dense bodies. Its activity increases 10 to 15-fold from the base to the tip of the villus. Morphometric analysis of the cell structureswhere this enzyme is localized reveals no marked changes in their relative proportions during crypt cell development. The expansion of the proliferative cell compartment along the whole length of the crypt which occurs during recovery after irradiation (72 hr after 400 rad X-irradiation) results in a marked reduction of alkaline phosphatase activity in the lower 10–15 cell positions at the base of the villus. During subsequent migration of these cells, the activity increases with cell age but normal values are not attained. From a morphometric analysis it was found that the ultrastructural development is similar to that in controls. These results suggest that during cell maturation, normal values for alkaline phosphatase activity are only attained after a 10–12 hr period of maturation in a non-proliferative state and only after the cell has migrated on to the functional villus compartment. In normal intestine, esterase activity shows a 3-fold increase from the bottom to the tip of the crypt and a 3 to 4-fold increase during migration up to the middle of the villus. Enzyme activity is localized in the endoplasmic reticulum, the dense bodies and the perinuclear space. Morphometric analyses reveal a 2 to 3-fold increase in the absolute size of these subcellular compartments during crypt cell differentiation and a 2-fold increase at the crypt-villus junction. The relative sizes increase 1.5-fold during crypt cell differentiation and at the time of transition of the cells on to the villus. Increased crypt cell proliferation after irradiation leads to a marked decrease in esterase activity both in crypts and villi. Morphometric analyses of electron micrographs indicate that these changes in activity are not related to any changes in the subcellular structures in which the enzyme is localized. It appears that the normal development of esterase activity depends both on the functional state of the cell and its localization in the crypt or villus.
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