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  • 1
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    Optimization and Performance of Silica-Based Media for Industrial-Scale Antibody Purification (2011)
    Wiley
    Details
    Publication Date: 2011-11-19
    Description: A silica-based medium with optimized pore size, pore volume, and coupling density was prepared for protein A affinity chromatography, to be used for industrial purification of IgG. Performance and durability of the silica-based medium were studied during repeated use and compared with other protein A media available on the market. The silica-based medium provided high dynamic binding capacities for human polyclonal IgG in the superficial liquid velocity range from 150 to 720 cm h –1 because of a high mass transfer coefficient and high mechanical strength. The dynamic binding capacity and IgG recovery were high and varied only slightly during 50–150 cycles of repeated use. The release of protein A ligand was low. A silica-based medium with optimized pore size, pore volume, and coupling density was developed for protein A affinity chromatography, to be used for industrial purification of IgG. Compared with common protein A media, it provided a high mass transfer rate and high dynamic binding capacities for IgG.
    Print ISSN: 0930-7516
    Electronic ISSN: 1521-4125
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Published by Wiley
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  • 2
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    A bacterium that degrades and assimilates poly(ethylene terephthalate) (2016)
    American Association for the Advancement of Science (AAAS)
    Details
    Publication Date: 2016-03-12
    Description: Poly(ethylene terephthalate) (PET) is used extensively worldwide in plastic products, and its accumulation in the environment has become a global concern. Because the ability to enzymatically degrade PET has been thought to be limited to a few fungal species, biodegradation is not yet a viable remediation or recycling strategy. By screening natural microbial communities exposed to PET in the environment, we isolated a novel bacterium, Ideonella sakaiensis 201-F6, that is able to use PET as its major energy and carbon source. When grown on PET, this strain produces two enzymes capable of hydrolyzing PET and the reaction intermediate, mono(2-hydroxyethyl) terephthalic acid. Both enzymes are required to enzymatically convert PET efficiently into its two environmentally benign monomers, terephthalic acid and ethylene glycol.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Yoshida, Shosuke -- Hiraga, Kazumi -- Takehana, Toshihiko -- Taniguchi, Ikuo -- Yamaji, Hironao -- Maeda, Yasuhito -- Toyohara, Kiyotsuna -- Miyamoto, Kenji -- Kimura, Yoshiharu -- Oda, Kohei -- New York, N.Y. -- Science. 2016 Mar 11;351(6278):1196-9. doi: 10.1126/science.aad6359.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Applied Biology, Faculty of Textile Science, Kyoto Institute of Technology, Matsugasaki, Sakyo-ku, Kyoto 606-8585, Japan. Department of Biosciences and Informatics, Keio University, 3-14-1 Hiyoshi, Kohoku-ku, Yokohama, Kanagawa 223-8522, Japan. ; Department of Applied Biology, Faculty of Textile Science, Kyoto Institute of Technology, Matsugasaki, Sakyo-ku, Kyoto 606-8585, Japan. ; Life Science Materials Laboratory, ADEKA, 7-2-34 Higashiogu, Arakawa-ku, Tokyo 116-8553, Japan. ; Department of Polymer Science, Faculty of Textile Science, Kyoto Institute of Technology, Matsugasaki, Sakyo-ku, Kyoto 606-8585, Japan. ; Ecology-Related Material Group Innovation Research Institute, Teijin, Hinode-cho 2-1, Iwakuni, Yamaguchi 740-8511, Japan. ; Department of Biosciences and Informatics, Keio University, 3-14-1 Hiyoshi, Kohoku-ku, Yokohama, Kanagawa 223-8522, Japan.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/26965627" target="_blank"〉PubMed〈/a〉
    Keywords: Amino Acid Sequence ; Betaproteobacteria/*enzymology ; Environmental Restoration and Remediation ; Enzymes/classification/genetics/metabolism ; Hydrolysis ; Microbial Consortia ; Molecular Sequence Data ; Phthalic Acids/metabolism ; Phylogeny ; Plastics/*metabolism ; Polyethylene Terephthalates/*metabolism ; Recycling
    Print ISSN: 0036-8075
    Electronic ISSN: 1095-9203
    Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics
    Published by American Association for the Advancement of Science (AAAS)
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  • 3
    Electronic Resource
    Electronic Resource
    An Automatic Ultrasonic Testing System for the Butt Weld Zone of Gas Pipe Lines (1996)
    s.l. ; Stafa-Zurich, Switzerland
    Materials science forum Vol. 210-213 (May 1996), p. 799-806 
    Details
    ISSN: 1662-9752
    Source: Scientific.Net: Materials Science & Technology / Trans Tech Publications Archiv 1984-2008
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Type of Medium: Electronic Resource
    URL: http://www.tib-hannover.de/fulltexts/2011/0528/02/01/transtech_doi~10.4028%252Fwww.scientific.net%252FMSF.210-213.799.pdf
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  • 4
    Electronic Resource
    Electronic Resource
    High energy high resolution monochromatic x-ray computed tomography using the Photon Factory vertical wiggler beamline : Proceedings of the 4th international conference on synchrotron radiation instrumentation (1992)
    [S.l.] : American Institute of Physics (AIP)
    Review of Scientific Instruments 63 (1992), S. 615-618 
    Details
    ISSN: 1089-7623
    Source: AIP Digital Archive
    Topics: Physics , Electrical Engineering, Measurement and Control Technology
    Notes: We have developed a high resolution monochromatic x-ray computed tomography system using synchrotron radiation in the energy range up to 50 keV. The system was installed in a vertical wiggler beamline (BL14C) at the Photon Factory of the National Laboratory for High Energy Physics, and consists of a double-crystal monochromator employing asymmetric Bragg reflections, a photodiode array detector with a phosphor screen, and a microcomputer for control. Computed tomography (CT) images with a pixel size of 6 to 8 μm and with a slice width ranging from 20 to 100 μm were obtained. An image subtraction technique for CT images above and below the x-ray absorption edge of iodine was also evaluated for liquid-containing glass capillaries. Since the CT value (pixel weighting) is linearly proportional to the concentration, the system can quantitatively evaluate the concentration distribution of iodine. These results indicate that the system is very useful for nondestructively evaluating fine structures and their constituent elements.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1063/1.1142668
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  • 5
    Electronic Resource
    Electronic Resource
    Labdane diterpenes from rhizomes of Hedychium coronarium
    Amsterdam : Elsevier
    Phytochemistry 37 (1994), S. 1383-1388 
    Details
    ISSN: 0031-9422
    Keywords: Hedychium coronarium ; Zingiberaceae ; labdane diterpenes.
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology , Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://linkinghub.elsevier.com/retrieve/pii/S0031-9422(00)90417-5
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  • 6
    Electronic Resource
    Electronic Resource
    Oxidation of N^2^1,N^2^2-etheno-bridged 5H-phlorins to the monocationic N^2^1, N^2^2-etheno-bridged 5-alkylporphyrins by Cu(Bf"4)2.
    Amsterdam : Elsevier
    Tetrahedron Letters 31 (1990), S. 5057-5060 
    Details
    ISSN: 0040-4039
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://linkinghub.elsevier.com/retrieve/pii/S0040-4039(00)97805-5
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  • 7
    Electronic Resource
    Electronic Resource
    High energy, high resolution monochromatic x-ray computed tomography system (1992)
    Springer
    Research in nondestructive evaluation 4 (1992), S. 55-78 
    Details
    ISSN: 1432-2110
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Abstract We have developed a high energy, high resolution monochromatic x-ray computed tomography system using synchrotron radiation. Our system is installed in a vertical wiggler beamline at the Photon Factory of the National Laboratory for High Energy Physics that can produce high brilliance x-rays in the high energy region. At the present stage, we have obtained monochromatic x-rays up to 75 keV, succeeded in taking computed tomography images of materials up to 50 keV with large absorption coefficients that could not be imaged by conventional methods, and observed three-dimensional fine structures in such materials. When the K-edge subtraction method was applied, the system could quantitatively and nondestructively measure the trace distribution of a specific element in the object of interest. In this paper we introduce the experimental system, mainly the crystal monochromator, evaluate the results, and discuss the performance of the system.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01625876
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  • 8
    Electronic Resource
    Electronic Resource
    Growth kinetics of animal cells immobilized within porous support particles in a perfusion culture (1994)
    Springer
    Applied microbiology and biotechnology 42 (1994), S. 531-535 
    Details
    ISSN: 1432-0614
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract The growth kinetics of anchorage-independent animal cells [mouse myeloma MPC-11 (ATCC CCL 167)] immobilized within porous polyvinyl formal resin biomass support particles (BSPs; 3×3×3 mm cubes; mean pore diameter, 60 μm) was analyzed by measuring intracellular and extracellular lactate dehydrogenase (LDH) activities in a perfusion culture. First, the intracellular LDH content of cells immobilized within the BSPs was assayed in a shake-flask culture and found to remain almost comparable to that of non-immobilized cells in the exponential growth phase under static culture. Then, cells inoculated in the BSPs were grown in a continuous stirred-tank bioreactor. Using the LDH content of non-immobilized cells, the density of immobilized cells and the numbers of leaked and dead cells were evaluated, respectively, by the intracellular LDH activity of immobilized and leaked cells and the LDH activity in cell-free culture supernatant. In the initial period, immobilized cells exhibited exponential growth at a constant apparent specific growth rate; however, the actual specific growth rate, which takes into consideration cell death and cell leakage, decreased significantly. In the stationary phase, the actual specific growth rate maintained a constant but markedly lower value than during exponential growth.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002530050289
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  • 9
    Electronic Resource
    Electronic Resource
    Growth kinetics of animal cells immobilized within porous support particles in a perfusion culture (1994)
    Springer
    Applied microbiology and biotechnology 42 (1994), S. 531-535 
    Details
    ISSN: 1432-0614
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract The growth kinetics of anchorage-independent animal cells [mouse myeloma MPC-11 (ATCC CCL 167) immobilized within porous polyvinyl formal resin biomass support particles (BSPs; 3 × 3 × 3 mm cubes; mean pore diameter, 60 µm) was analyzed by measuring intracellular and extracellular lactate dehydrogenase (LDH) activities in a perfusion culture. First, the intracellular LDH content of cells immobilized within the BSPs was assayed in a shake-flask culture and found to remain almost comparable to that of non-immobilized cells in the exponential growth phase under static culture. Then, cells inoculated in the BSPs were grown in a continuous stirred-tank bioreactor. Using the LDH content of non-immobilized cells, the density of immobilized cells and the numbers of leaked and dead cells were evaluated, respectively, by the intracellular LDH activity of immobilized and leaked cells and the LDH activity in cell-free culture supernatant. In the initial period, immobilized cells exhibited exponential growth at a constant apparent specific growth rate; however, the acutal specific growth rate, which takes into consideration cell death and cell leakage, decreased significantly. In the stationary phase, the actual specific growth rate maintained a constant but markedly lower value than during exponential growth.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00173916
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  • 10
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    High energy high resolution monochromatic x‐ray computed tomography using the Photon Factory vertical wiggler beamline (1992)
    American Institute of Physics
    Details
    Publication Date: 1992-01-01
    Print ISSN: 0034-6748
    Electronic ISSN: 1089-7623
    Topics: Electrical Engineering, Measurement and Control Technology , Physics
    Published by American Institute of Physics
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