Springer Online Journal Archives 1860-2000
Chemistry and Pharmacology
Energy, Environment Protection, Nuclear Power Engineering
Abstract The paper describes a solid-phase radioimmunoassay for triiodothyronine (T3) using antibody coupled to carboxymethylcellulose powder. The free carboxylic acid groups of cellulose are covalently coupled to the amino groups of the antibody using 1-ethyl-3-(3-dimethylamino-propyl) carbodiimide hydrochloride. Immobilized antibody thus prepared was used in a solid-phase radioimmunoassay of T3. The assay has a sensitivity of 0.18 μg/l, and a range of 0.18–4 μg/l. Satisfactory correlation was obtained when this assay was compared with a T3 assay based on dextran coated charcoal separation system (Y=0.95X+0.15 μg/L; r=0.98).
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