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  • 1
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    IODP Expedition 337, Shimokita Deep Coalbed Biosphere (2015)
    PANGAEA
    In:  Supplement to: Inagaki, F; Hinrichs, Kai-Uwe; Kubo, Y; Bowles, Marshall W; Heuer, Verena B; Hong, W-L; Hoshino, Tatsuhiko; Ijiri, Akira; Imachi, H; Ito, M; Kaneko, Masanori; Lever, Mark A; Lin, Yu-Shih; Methe, B A; Morita, S; Morono, Yuki; Tanikawa, Wataru; Bihan, M; Bowden, Stephen A; Elvert, Marcus; Glombitza, Clemens; Gross, D; Harrington, G J; Hori, T; Li, K; Limmer, D; Liu, Chiung-Hui; Murayama, M; Ohkouchi, Naohiko; Ono, Shuhei; Park, Young-Soo; Phillips, S C; Prieto-Mollar, Xavier; Purkey, M; Riedinger, Natascha; Sanada, Yoshinori; Sauvage, J; Snyder, Glen T; Susilawati, R; Takano, Yoshinori; Tasumi, E; Terada, Takeshi; Tomaru, Hitoshi; Trembath-Reichert, E; Wang, D T; Yamada, Y (2015): Exploring deep microbial life in coal-bearing sediment down to ~2.5 km below the ocean floor. Science, 439 (6246), 420-424, https://doi.org/10.1126/science.aaa6882
    Details
    Publication Date: 2023-04-29
    Description: Microbial life inhabits deeply buried marine sediments, but the extent of this vast ecosystem remains poorly constrained. Here we provide evidence for the existence of microbial communities in ~40° to 60°C sediment associated with lignite coal beds at ~1.5 to 2.5 km below the seafloor in the Pacific Ocean off Japan. Microbial methanogenesis was indicated by the isotopic compositions of methane and carbon dioxide, biomarkers, cultivation data, and gas compositions. Concentrations of indigenous microbial cells below 1.5 km ranged from 〈10 to ~10**4 cells cm**-3. Peak concentrations occurred in lignite layers, where communities differed markedly from shallower subseafloor communities and instead resembled organotrophic communities in forest soils. This suggests that terrigenous sediments retain indigenous community members tens of millions of years after burial in the seabed.
    Keywords: Integrated Ocean Drilling Program / International Ocean Discovery Program; IODP
    Type: Dataset
    Format: application/zip, 2 datasets
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    DATA PANGAEA
  • 2
    Electronic Resource
    Electronic Resource
    Chronopotentiometric and Chronocoulometric Measurements of Adsorption of Lead and Mercury(II) at Mercury Electrodes. (1966)
    s.l. : American Chemical Society
    Analytical chemistry 38 (1966), S. 392-404 
    Details
    ISSN: 1520-6882
    Source: ACS Legacy Archives
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1021/ac60235a006
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  • 3
    Electronic Resource
    Electronic Resource
    Inhibition of Electrode Reactions by Adsorbed Lead Iodide, Bromide, and Thiocyanate. (1966)
    s.l. : American Chemical Society
    Analytical chemistry 38 (1966), S. 405-409 
    Details
    ISSN: 1520-6882
    Source: ACS Legacy Archives
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1021/ac60235a007
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  • 4
    Electronic Resource
    Electronic Resource
    On the Sublimation of Amino Acids and Peptides (1955)
    s.l. : American Chemical Society
    Journal of the American Chemical Society 77 (1955), S. 1678-1680 
    Details
    ISSN: 1520-5126
    Source: ACS Legacy Archives
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1021/ja01611a085
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  • 5
    Electronic Resource
    Electronic Resource
    Cross-linking of cardiac gap junction connexons by thiol/disulfide exchanges (1989)
    Springer
    The journal of membrane biology 108 (1989), S. 247-252 
    Details
    ISSN: 1432-1424
    Keywords: cardiac gap junctions ; thiol/disulfide exchanges
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Summary SDS-polyacrylamide gel electrophoresis and immunoblotting were used to investigate inter- and intramolecular disulfide bonds to connexin 43 (the cardiac gap junctional protein) in isolated rat heart gap junctions and in whole heart fractions. In gap junctions isolated in the absence of alkylating agent, connexin 43 molecules are cross-linked by disulfide bonds. The use of iodoacetamide (100mm) for the first steps of isolation procedure prevents the formation of these artifactual linkages. Investigation of connexin 43 in whole heart fractions by means of antibodies confirms the results obtained with isolated gap junctions; that is, connexin 43 molecules are not interconnected with disulfide bridges. In whole heart fractions treated with alkylating agents, a 38 kD protein, immunologically related to connexin 43, and containing intramolecular disulfide bonds is detected. It is hypothesized that this protein might be a folded form of connexin 43, a precursory form of the molecules embedded in the gap junctions.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01871739
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  • 6
    Electronic Resource
    Electronic Resource
    Beiträge zur Biosynthese von Nicotinsäure und Chinolinsäure bei Mycobacterium bovis Stamm BCG (1970)
    Springer
    Archives of microbiology 75 (1970), S. 80-88 
    Details
    ISSN: 1432-072X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Description / Table of Contents: Zusammenfassung Zur Untersuchung der Nicotinsäurebiosynthese werden an wachsenden Kulturen von Mycobacterium bovis Stamm BCG Einbauversuche mit 14C-markierten Precursoren durchgeführt und die spezifischen Inkorporationsraten sowie die 14C-Verteilung der isolierten Nicotinsäure bestimmt. Formiat-(14) und D-Ribose-(U-14C) werden nicht in Nicotinsäure eingebaut. Im Gegensatz zu Befunden anderer Autoren an Clostridium butylicum zeigen Formiat-(14C) und Acetat-(14C) im Rohhomogenat von BCG-Bakterien keinen Einbau in Nicotinsäure und sind als Nicotinsäurevorstufen auszuschließen. Glycerin-(1,3-14C) und Glycerinsäure-(3-14C) gehen bevorzugt in die C-Atome 4, 5 und 6. Das C-Atom 6 der Nicotinsäure entspricht dem C-3 der Glycerinsäure. Somit erfolgt der Einbau der Glycerinsäure nicht über ein symmetrisches Intermediärprodukt. Durch Isotopen verdünnungsanalyse wird Chinolinsäure als Ausscheidungsprodukt von M. bovis Stamm BCG nachgewiesen. Ihre Biosynthese erfolgt in Analogie zur Nicotinsäurebildung aus Aspartat und Glycerin.
    Notes: Summary Biogenesis of nicotinic acid was studied by feeding 14C-labelled precursors in growing cultures of Mycobacterium bovis strain BCG. The incorporation rate as well as the distribution of 14C in the isolated nicotinic acid were determined. Formate-(14C) and D-ribose-(U-14C) were not incorporated into nicotinic acid. In contrast to experiments performed with Clostridium butylicum, crude homogenates of M. bovis strain BCG showed no incorporation of formate-(14C) and acetate-(14C) under the experimental conditions applied and therefore, these compounds should be excluded as possible precursors. Glycerol-(1,3-14C) and glyceric acid-(3-14C) are incorporated into positions 4, 5 and 6, as expected. The carbon atom 6 of nicotinic acid was almost exclusively derived from C-3 of glyceric acid. Incorporation of glyceric acid therefore does not follow through a symmetrical intermediate. By isotope dilution technique quinolinic acid has been found in the culture medium indicating to be an excretary product of M. bovis strain BCG. Its biosynthesis occurs, in analogy to nicotinic acid formation, from aspartate and glycerol.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00412095
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  • 7
    Electronic Resource
    Electronic Resource
    Influence of repeated oral doses of ethinyloestradiol on the metabolic disposition of [13C2]-ethinyloestradiol in young women (1996)
    Springer
    European journal of clinical pharmacology 50 (1996), S. 231-235 
    Details
    ISSN: 1432-1041
    Keywords: Key words Ethinyloestradiol; clearance ; stable isotope
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology , Medicine
    Notes: Abstract Objective: To examine the influence of daily oral administration of ethinyloestradiol on the total clearance of 13C-labeled ethinyloestradiol in women. Methods: 19, healthy, young women received a single IV dose of 0.06 mg 13C-ethinyloestradiol. Subsequently, they were treated with daily oral doses of 0.06 mg ethinyloestradiol for 8 days. On the last day of oral treatment, they received a further IV dose of 0.06 mg 13C-ethinyloestradiol. The pharmacokinetic parameters clearance, area under the serum level-time curve, terminal half-life, steady-state volume of distribution and mean residence time of 13C-ethinyloestradiol in each volunteer were evaluated after both IV doses, and the corresponding pairs of parameters were examined statistically for the significance of intraindividual differences. Results: Following the first (second) intravenous administration, the mean area under the curve was 2.54 (2.67) ng⋅h⋅ml−1. The terminal half-life and mean residence time were 9.7 (9.6) h and 10.5 (10.1) h, respectively. The steady-state volume of distribution was 4.3 (3.9) l⋅kg−1 and the clearance was 7.0 (6.6) ml⋅ min−1⋅kg−1. No significant difference was observed in any of these parameters between the first and the second IV doses of 13C-EE2. Conclusions: Since the clearance in particular remained unchanged after repeated oral administration of ethinyloetradiol, the hypothesis that ethinyloestradiol can inhibit its own metabolism in vivo can be rejected.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002280050098
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  • 8
    Electronic Resource
    Electronic Resource
    Effects of Substance P on Secretion of Catecholamines from Populations of Bovine Chromaffin Cells and on Calcium Transients in Individual Cells (1991)
    Oxford, UK : Blackwell Publishing Ltd
    Annals of the New York Academy of Sciences 632 (1991), S. 0 
    Details
    ISSN: 1749-6632
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Natural Sciences in General
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1749-6632.1991.tb33112.x
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  • 9
    Electronic Resource
    Electronic Resource
    High-Voltage Paper Electrophoresis of Aliphatic Amines and Related Compounds (1959)
    [s.l.] : Nature Publishing Group
    Nature 184 (1959), S. 1633-1634 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] The multitude of amines potentially present makes their separation and reliable identification in unknown mixtures quite difficult. The application of high potential gradients with attendant increased resolving power and high degree of reproducibility offers obvious advantages. The design of ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/1841633a0
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  • 10
    Electronic Resource
    Electronic Resource
    High-Voltage Paper Electrophoresis of Inorganic Cations: Alkali, Alkaline-Earth and Other Metals (1957)
    [s.l.] : Nature Publishing Group
    Nature 180 (1957), S. 596-598 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] THE importance of the alkali and alkaline-earth metals in biochemistry, nuclear science and industry has prompted suggestions for convenient micro-methods for the detection and estimation of these ions in mixtures or by themselves. Most of the methods recently proposed are based on ! m" K ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/180596a0
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