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  • 1
    Electronic Resource
    Electronic Resource
    The HIC signalling pathway links CO 2 perception to stomatal development (2000)
    [s.l.] : Macmillian Magazines Ltd.
    Nature 408 (2000), S. 713-716 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] Stomatal pores on the leaf surface control both the uptake of CO2 for photosynthesis and the loss of water during transpiration. Since the industrial revolution, decreases in stomatal numbers in parallel with increases in atmospheric CO2 concentration have provided ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/35047071
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  • 2
    Electronic Resource
    Electronic Resource
    Self-incompatibility in Nicotiana alata involves degradation of pollen rRNA (1990)
    [s.l.] : Nature Publishing Group
    Nature 347 (1990), S. 757-760 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] The finding that style S-glycoproteins from N. alata are RNases11 leads to the question of whether this enzymatic function has a role in expression of self-incompatibility (SI). To approach this question we obtained labelled pollen by growing plants in the presence of 32P and followed the fate of ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/347757a0
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  • 3
    Electronic Resource
    Electronic Resource
    The manipulation and modification of tomato fruit ripening by expression of antisense RNA in transgenic plants (1955)
    Springer
    Euphytica 85 (1955), S. 193-202 
    Details
    ISSN: 1573-5060
    Keywords: carotenoids ; ethylene ; gene expression ; Lycopersicon esculentum Mill. ; polygalacturonase ; pectinesterase ; phytoene synthase ; ACC oxidase
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The common cultivated tomato (Lycopersicon esculentum Mill.) provides a major focus for improvement of crop quality through genetic engineering. Identification of ripening-related cDNAs has enabled the modification of specific aspects of ripening by manipulating gene expression in transgenic plants. By utilizing ‘antisense RNA’ to modify expression of ripening genes, we have inhibited the production of the cell wall-metabolising enzymes polygalacturonase and pectinesterase and created transgenic plants that contain, effectively, single, targeted mutations affecting these genes. Furthermore, this approach has been used with previously unidentified cDNA clones to enable both functional identification and manipulation of genes involved in ethylene production (ACC oxidase) and carotenoid biosynthesis (phytoene synthase). The use of antisense RNA targeted to specific genes to alter ripening phenotypes and improve commercial utility of fruit by affecting shelf-life, processing characteristics and nutritional content is discussed. We have used the extreme ripening-impaired mutant, ripening inhibitor (rin) to identify additional genes implicated in the ripening process. This approach has resulted in the cloning of several novel ripening-related mRNAs which are now being studied by antisense experiments. This may enable identification and manipulation of additional genes involved in processes such as softening, flavour and aroma generation and susceptibility to pathogens.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00023948
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  • 4
    Electronic Resource
    Electronic Resource
    A role for glutamate decarboxylase during tomato ripening: the characterisation of a cDNA encoding a putative glutamate decarboxylase with a calmodulin-binding site (1995)
    Springer
    Plant molecular biology 27 (1995), S. 1143-1151 
    Details
    ISSN: 1573-5028
    Keywords: differential screening ; gene expression ; Lycopersicon esculentum ; rin ; ripening inhibitor
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A tomato fruit cDNA library was differentially screened to identify mRNAs present at higher levels in fruit of the tomato ripening mutant rin (ripening inhibitor). Complete sequencing of a unique clone ERT D1 revealed an open reading frame with homology to several glutamate decarboxylases. The deduced polypeptide sequence has 80% overall amino acid sequence similarity to a Petunia hybrida glutamate decarboxylase (petGAD) which carries a calmodulin-binding site at its carboxyl terminus and ERT D1 appears to have a similar domain. ERT D1 mRNA levels peaked at the first visible sign of fruit colour change during normal tomato ripening and then declined, whereas in fruit of the ripening impaired mutant, rin, accumulation of this mRNA continued until at least 14 days after the onset of ripening. This mRNA was present at much lower levels in other tissues, such as leaves, roots and stem, and was not increased by wounding. Possible roles for GAD, and its product γ-aminobutyric acid (GABA) in fruit, are discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00020887
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  • 5
    Electronic Resource
    Electronic Resource
    Expression of a proteasome α-type subunit gene during tobacco development and senescence (1999)
    Springer
    Plant molecular biology 39 (1999), S. 325-333 
    Details
    ISSN: 1573-5028
    Keywords: cDNA ; cell cycle ; expression ; mRNA ; protein degradation ; senescence
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Proteasomes degrade specific proteins that have been targeted for proteolysis by ubiquitination. In animals and yeast nuclear-localised proteasomes play a role in regulating the cell cycle, and other developmental processes, via control of the levels of regulatory nuclear proteins such as cyclins and transcription factors. A cDNA, NtPSA1, isolated from tobacco styles was found to have high similarity to human and yeast genes, PRCI−human and PRCI−yeast with 63.4% and 51.6% overall identity respectively. These genes are believed to encode non-catalytic α-type subunits of 26S proteasomes and like NtPSA1 have putative nuclear localisation signals. NtPSA1 RNA was found to accumulate to varying levels in different parts of the plant and at different developmental stages. In particular, the level of NtPSA1 RNA was high in young dividing and expanding tissues, and declined during the senescence of both leaves and flowers. These results suggest that a role of proteasomes in plant nuclei may be to regulate developmental events by controlling the levels of regulatory proteins in proliferating and developing tissues, rather than to degrade and recycle proteins during senescence.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1006102110889
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  • 6
    Electronic Resource
    Electronic Resource
    Control and manipulation of gene expression during tomato fruit ripening (1989)
    Springer
    Plant molecular biology 13 (1989), S. 303-311 
    Details
    ISSN: 1573-5028
    Keywords: fruit ripening ; tomatoes ; polygalacturonase ; antisense
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Ripening is a complex developmental process involving changes in the biochemistry, physiology and gene expression of the fruit. It is an active process characterised by changes in all cellular compartments. cDNA cloning has been used as an approach to analyse changes in gene expression during fruit ripening. This has revealed that several genes are switched on specifically during fruit ripening, including one encoding polygalacturonase (PG), a major cell wall protein. These cDNA clones have been used to study the expression of the genes in normal and ripening mutant fruits, and under environmental stress conditions. The PG gene has been isolated and it has been demonstrated that 1450 bases 5′ of the coding region are sufficient for the tissue- and development-specific expression of a bacterial marker gene in transgenic tomatoes. Antisense RNA techniques have been developed to generate novel mutant tomatoes in which the biochemical function of this enzyme and its involvement in fruit softening has been tested.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00025318
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  • 7
    Electronic Resource
    Electronic Resource
    Inheritance and effect on ripening of antisense polygalacturonase genes in transgenic tomatoes (1990)
    Springer
    Plant molecular biology 14 (1990), S. 369-379 
    Details
    ISSN: 1573-5028
    Keywords: antisense ; polygalacturonase ; ripening ; RNA ; tomatoes ; transgenic
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The role of the cell wall hydrolase polygalacturonase (PG) during fruit ripening was investigated using novel mutant tomato lines in which expression of the PG gene has been down regulated by antisense RNA. Tomato plants were transformed with chimaeric genes designed to express anti-PG RNA constitutively. Thirteen transformed lines were obtained of which five were analysed in detail. All contained a single PG antisense gene, the expression of which led to a reduction in PG enzyme activity in ripe fruit to between 5% and 50% that of normal. One line, GR16, showed a reduction to 10% of normal PG activity. The reduction in activity segregated with the PG antisense gene in selfed progeny of GR16. Plants homozygous for the antisense gene showed a reduction of PG enzyme expression of greater than 99%. The PG antisense gene was inherited stably through two generations. In tomato fruit with a residual 1% PG enzyme activity pectin depolymerisation was inhibited, indicating that PG is involved in pectin degradation in vivo. Other ripening parameters, such as ethylene production, lycopene accumulation, polyuronide solubilisation, and invertase activity, together with pectinesterase activity were not affected by the expression of the antisense gene.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00028773
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  • 8
    Electronic Resource
    Electronic Resource
    A histidine decarboxylase-like mRNA is involved in tomato fruit ripening (1993)
    Springer
    Plant molecular biology 23 (1993), S. 627-631 
    Details
    ISSN: 1573-5028
    Keywords: ethylene ; histamine ; gene expression ; Lycopersicon esculentum ; ripening mutant ; ripening inhibitor
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract DNA sequencing of a tomato ripening-related cDNA, TOM 92, revealed an open reading frame with homology to several pyridoxal 5′-phosphate histidine decarboxylases, containing the conserved amino acid residues known to bind pyridoxal phosphate and α-fluoromethylhistidine, an inhibitor of enzyme activity. TOM 92 mRNA accumulated during early fruit ripening and then declined. Fruit of the ripeningimpaired tomato mutant, ripening inhibitor (rin), did not accumulate TOM 92 mRNA, and its accumulation was not restored by treatment of fruit with ethylene. The TOM 92 mRNA was not detected in tomato leaves and unripe fruit.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00019310
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  • 9
    Electronic Resource
    Electronic Resource
    Pollination-enhanced expression of a receptor-like protein kinase related gene in tobacco styles (1997)
    Springer
    Plant molecular biology 33 (1997), S. 653-665 
    Details
    ISSN: 1573-5028
    Keywords: cDNA ; pollination ; protein kinase ; receptor-like related ; style ; tobacco
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract We have isolated a putative serine/threonine receptor kinase gene with an expression pattern indicating that it may play a role in the stylar response to pollination. Differential display PCR was used to select tobacco mRNAs with increased accumulation following pollination. NTS16, a cDNA identified by this method, is homologous to a ca. 2.4 kb mRNA primarily expressed in pistil tissues. Levels of this mRNA increase during floral development and are further increased by pollination reaching maximal accumulation 12–18 hours after pollination and then declining. mRNA levels can also be increased by the application of ethylene to unpollinated flowers. A polypeptide encoded by the NTS16 open reading frame has sequence similarity to the catalytic domain of several receptor protein kinases from plants including the S-receptor kinases implicated in the rejection of self-pollen in Brassica species and the Pto gene product of tomato which confers resistance to a bacterial pathogen.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1005787612041
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  • 10
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    Models and Mechanisms of Stomatal Mechanics (2018)
    Cell Press
    Details
    Publication Date: 2018-09-01
    Print ISSN: 1360-1385
    Electronic ISSN: 1878-4372
    Topics: Biology , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Published by Cell Press
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