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  • 1
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    Biological and Chemical Oceanography Data Management Office (BCO-DMO). Contact: bco-dmo-data@whoi.edu
    Publication Date: 2020-01-08
    Description: Dataset: mims_to_irms
    Description: In order to ensure that 15N2 atom% effectuated by Membrane Inlet Mass Spectrometry, we tested whether the mass-specific signal ratios returned by MIMS are equivalent with parallel measurements on a Isotope Ratio Mass Spectrometer, on the basis that the latter returns accurate ratios (see 778000 cal_iso). MIMS and IRMS returned identical values for parallel samples. For a complete list of measurements, refer to the full dataset description in the supplemental file 'Dataset_description.pdf'. The most current version of this dataset is available at: https://www.bco-dmo.org/dataset/778065
    Description: NSF Division of Ocean Sciences (NSF OCE) OCE-1732246
    Repository Name: Woods Hole Open Access Server
    Type: Dataset
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  • 2
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    Biological and Chemical Oceanography Data Management Office (BCO-DMO). Contact: bco-dmo-data@whoi.edu
    Publication Date: 2020-01-08
    Description: Dataset: dis_15N2_gas
    Description: In order to verify that measurements of the 15N2 atom% of dissolved N2 gas samples effectuated by Membrane Inlet Mass Spectrometry are accurate, we developed a specific protocol to prepare dissolved 15N2 gas standards. We detail the protocol herein, and report representative MIMS measurements of the prepared standards. For a complete list of measurements, refer to the full dataset description in the supplemental file 'Dataset_description.pdf'. The most current version of this dataset is available at: https://www.bco-dmo.org/dataset/778021
    Description: NSF Division of Ocean Sciences (NSF OCE) OCE-1732246
    Repository Name: Woods Hole Open Access Server
    Type: Dataset
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  • 3
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    Biological and Chemical Oceanography Data Management Office (BCO-DMO). Contact: bco-dmo-data@whoi.edu
    Publication Date: 2020-01-08
    Description: Dataset: trial_b
    Description: The “dissolution” method to measure N2 fixation rates with 15N2 gas tracer involves the preparation of 15N2-enriched water that is then added to each incubation bottle. Investigators typically measure the 15N2 atom% of the 15N2-enriched inoculum by MIMS, and extrapolate the 15N2 atom% in the incubations based on the inoculum value. Here, we demonstrate that such extrapolation yields inaccurate estimates of the 15N2 atom% of incubations. The latter should be measured directly. For a complete list of measurements, refer to the full dataset description in the supplemental file 'Dataset_description.pdf'. The most current version of this dataset is available at: https://www.bco-dmo.org/dataset/778158
    Description: NSF Division of Ocean Sciences (NSF OCE) OCE-1732246
    Repository Name: Woods Hole Open Access Server
    Type: Dataset
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  • 4
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    Biological and Chemical Oceanography Data Management Office (BCO-DMO). Contact: bco-dmo-data@whoi.edu
    Publication Date: 2020-01-08
    Description: Dataset: integrity_tests
    Description: We monitored the 15N2 gas atom% values of prepared solutions stored in Exetainer vials (filled with no headspace) in order to verify that these values remained stable over a period of weeks to months of storage. The integrity of samples was preserved for at least 6 months. For a complete list of measurements, refer to the full dataset description in the supplemental file 'Dataset_description.pdf'. The most current version of this dataset is available at: https://www.bco-dmo.org/dataset/778047
    Description: NSF Division of Ocean Sciences (NSF OCE) OCE-1732246
    Repository Name: Woods Hole Open Access Server
    Type: Dataset
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  • 5
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    Biological and Chemical Oceanography Data Management Office (BCO-DMO). Contact: bco-dmo-data@whoi.edu
    Publication Date: 2020-01-08
    Description: Dataset: trial_a
    Description: The “dissolution” method to measure N2 fixation rates with 15N2 gas tracer involves the preparation of 15N2-enriched water that is then added to each incubation bottle. Investigators typically measure the 15N2 atom% of the 15N2-enriched inoculum by MIMS, and extrapolate the 15N2 atom% in the incubations based on the inoculum value. Here, we demonstrate that such extrapolation yields inaccurate estimates of the 15N2 atom% of incubations. The latter should be measured directly. For a complete list of measurements, refer to the full dataset description in the supplemental file 'Dataset_description.pdf'. The most current version of this dataset is available at: https://www.bco-dmo.org/dataset/778126
    Description: NSF Division of Ocean Sciences (NSF OCE) OCE-1732246
    Repository Name: Woods Hole Open Access Server
    Type: Dataset
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  • 6
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    Biological and Chemical Oceanography Data Management Office (BCO-DMO). Contact: bco-dmo-data@whoi.edu
    Publication Date: 2020-01-08
    Description: Dataset: cal_iso
    Description: In order to verify that measurements of the 15N2 atom% of dissolved N2 gas samples effectuated by Membrane Inlet Mass Spectrometry are accurate, we compared these to corresponding measurements of parallel samples on a Delta V Isotope Ratio Mass Spectrometer (IRMS). To this end, we first ensured that the IRMS returns accurate 15N2 atom% values, by measuring the 15N2 atom% of a serial dilutions of a 15N2 gas stock in air and helium. This calibration is documented herein, showing that IRMS measurements in the experimental range were accurate, as expected For a complete list of measurements, refer to the full dataset description in the supplemental file 'Dataset_description.pdf'. The most current version of this dataset is available at: https://www.bco-dmo.org/dataset/778000
    Description: NSF Division of Ocean Sciences (NSF OCE) OCE-1732246
    Repository Name: Woods Hole Open Access Server
    Type: Dataset
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  • 7
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    Biological and Chemical Oceanography Data Management Office (BCO-DMO). Contact: bco-dmo-data@whoi.edu
    Publication Date: 2020-01-08
    Description: Dataset: mz_30_int_from_o2
    Description: Molecular oxygen (O2) has been documented to ionize partially to NO+ in mass spectrometer sources, generating a signal that overlaps with that of 15N2 (mass-to-charge of 30). Here, we tested whether O2 needs to be specifically removed from the sample streams of the Membrane Inlet Mass Spectrometer and the Isotope Ratio Mass Spectrometer in order to properly quantitate 15N2 atom% at pertinent concentrations. Sample streams with and without O2 yielded similar signals, suggesting that O2 does not interfere with the 15N2 atom% measurements in the experimental range with the given instruments. For a complete list of measurements, refer to the full dataset description in the supplemental file 'Dataset_description.pdf'. The most current version of this dataset is available at: https://www.bco-dmo.org/dataset/778083
    Description: NSF Division of Ocean Sciences (NSF OCE) OCE-1732246
    Repository Name: Woods Hole Open Access Server
    Type: Dataset
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  • 8
    ISSN: 1574-6941
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Prokaryotic microbes play a critical role in oceanic Fe cycling. They contain most of the biogenic Fe in offshore waters and are responsible for a large portion of the Fe uptake by the plankton community. In the subarctic North Pacific, surface populations of heterotrophic species assimilate more than 50% of the dissolved Fe and thus compete directly with phytoplankton for this limiting resource. In oligotrophic tropical and subtropical waters, photosynthetic bacteria become more important in Fe cycling as the number of unicellular cyanobacteria increases and the nitrogen-fixing Trichodesmium, which contains most of the biogenic Fe in the mixed layer, becomes abundant. Like their terrestrial counterparts, heterotrophic and phototrophic marine bacteria produce Fe-binding siderophores that are involved in Fe acquisition. Evidence exists that bacteria may modify Fe chemistry in the sea through the production of these ligands and thereby play a significant role in regulating production of eukaryotic phytoplankton.
    Type of Medium: Electronic Resource
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  • 9
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    PANGAEA
    In:  Supplement to: Granger, Julie; Boshers, Danielle S; Böhlke, John Karl; Yu, Dan; Chen, Nengwang; Tobias, Craig R (2020): The influence of sample matrix on the accuracy of nitrite N and O isotope ratio analyses with the azide method. Rapid Communications in Mass Spectrometry, 34(1), https://doi.org/10.1002/rcm.8569
    Publication Date: 2020-02-12
    Description: Experiments demonstrating matrix effects (sample salinity and pH) on nitrite N and O isotope ratio measurements with the azide method of McIlvin and Altabet (2005, doi:10.1021/ac050528s).
    Type: Dataset
    Format: application/zip, 34.0 kBytes
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  • 10
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    PANGAEA
    In:  Supplement to: Lehmann, Nadine; Granger, Julie; Kienast, Markus; Brown, Kevin S; Rafter, Patrick A; Martínez Méndez, Gema; Mohtadi, Mahyar (2018): Isotopic evidence for the evolution of subsurface nitrate in the Western Equatorial Pacific. Journal of Geophysical Research: Oceans, 123(3), 1684-1707, https://doi.org/10.1002/2017JC013527
    Publication Date: 2020-02-22
    Description: Subsurface waters from both hemispheres converge in the Western Equatorial Pacific (WEP), some of which form the Equatorial Undercurrent (EUC) that influences equatorial Pacific productivity across the basin. Measurements of nitrogen (N) and oxygen (O) isotope ratios in nitrate (d15N-NO3 and d18O-NO3), the isotope ratios of dissolved inorganic carbon (d13C-DIC), and complementary biogeochemical tracers reveal that northern and southern WEP waters have distinct biogeochemical histories. Organic matter remineralization plays an important role in setting the nutrient characteristics on both sides of the WEP. However, remineralization in the northern WEP contributes a larger concentration of the nutrients, consistent with the older "age" of northern thermocline- and intermediate-depth waters. Remineralization introduces a relatively low d15N-NO3 to northern waters, suggesting the production of sinking organic matter by N2 fixation at the surface - consistent with the notion that N2 fixation is quantitatively important in the North Pacific. In contrast, remineralization contributes elevated d15N-NO3 to the southern WEP thermocline, which we hypothesize to derive from the vertical flux of high-d15N material at the southern edge of the equatorial upwelling. This signal potentially masks any imprint of N2 fixation from South Pacific waters. The observations further suggest that the intrusion of high d15N-NO3 and d18O-NO3 waters from the eastern margins is more prominent in the northern than southern WEP. Together, these north-south differences enable the examination of the hemispheric inputs to the EUC, which appear to derive predominantly from southern hemisphere waters.
    Type: Dataset
    Format: text/tab-separated-values, 88998 data points
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