ISSN:
1432-2242
Keywords:
Key words Pisum sativum
;
Linkage mapping
;
DNA markers
;
Expressed sequence tags
;
Sequence-tagged sites
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract A linkage map of the pea (Pisum sativum L.) genome is presented which is based on F2 plants produced by crossing the marrowfat cultivar ‘Primo’ and the blue-pea breeding line ‘OSU442-15’. This linkage map consists of 209 markers and covers 1330 cM (Kosambi units) and includes RFLP, RAPD and AFLP markers. By mapping a number of anchor loci, the ‘Primo’בOSU442-15’ map has been related to other pea linkage maps. A feature of the map is the incorporation of 29 loci representing genes of known function, obtained from other laboratories. The map also contains RFLP loci detected using sequence-characterized cDNA clones developed in our laboratory. The putative identities of 38 of these cDNA clones were assigned by examining public-sequence databases for protein or nucleotide-sequence similarities. The conversion of sequence-characterized pea cDNAs into PCR-amplifiable and polymorphic sequence-tagged sites (STSs) was investigated using 18 pairs of primers designed for single-copy sequences. Eleven polymorphic STSs were developed.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/s001220050695
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