ISSN:
1432-2242
Keywords:
Vigna aconitifolia
;
Co-cultivation
;
Agrobacterium tumefaciens
;
Kanamycin resistance
;
Nopaline synthesis
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Summary A simplified protoplast regeneration system for Vigna aconitifolia was developed. A plating efficiency of 60% was obtained using mesophyll protoplasts from 10-day-old seedlings. By co-cultivation of protoplasts with Agrobacterium tumefaciens containing the Ti plasmid derivative pGV 3850∶1103 neo kanamycin-resistant colonies were obtained; 23% of the transformed lines showed expression of the nonselected co-transferred nopaline synthase gene. Transformation was confirmed by Southern blot analysis using a nonradioactive detection system. The plant cultivar used was an important factor in determining transformation frequencies since one of the cultivars had an 85 fold higher transformation rate than the other.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00249165
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