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  • 1
    Electronic Resource
    Electronic Resource
    Multiple reconstruction of barley karyotype resulting in complete cytological marking of the chromosome complement (1989)
    Springer
    Theoretical and applied genetics 78 (1989), S. 683-688 
    Details
    ISSN: 1432-2242
    Keywords: Reciprocal translocation ; Pericentric inversion ; Giemsa banding ; Barley
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A new reconstructed barley karyotype, PK88, which is a quadruple homozygote for three unequal translocations, 1–2, 3–4, 5–7, and one pericentric inversion in chromosome 6, was studied. As a result of these chromosome rearrangements, a complete cytological marking of the complement has been achieved. Due to the specific intra or interchromosomal transfer of particular bands, Giemsa staining of somatic chromosomes provided clear-cut indications about the localization of translocation and inversion breakpoints. It was established that the long arms of chromosomes 1, 2, 4, 5 and 7 and the short arm of chromosome 3 have been involved in interchanges 1–2, 3–4, and 5–7. The breakpoints of pericentric inversion proved to be located proximally to the short (satellite) arm and distally in the long arm of chromosome 6. PK-88 offers an essential gain in resolution power and extension of the areas of application in cytogenetics over other reconstructed karyotypes produced so far in barley.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00262564
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  • 2
    Electronic Resource
    Electronic Resource
    Further evidence for a high position-specific effect in the action of chemical mutagens on the chromosomes of barley (1991)
    Springer
    Theoretical and applied genetics 83 (1991), S. 179-184 
    Details
    ISSN: 1432-2242
    Keywords: Chromosome aberrations ; Translocation karyotypes ; Maleic hydrazide ; Ethyl methanesulphonate ; Hordeum vulgare
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Further convincing evidence for the decisive role of chromosome constitution in the processes underlying the specific induction of structural mutations by chemical mutagens is described. The most important conclusion to be drawn from the results obtained in experiments with maleic hydrazide (MH) and ethyl methanesulphonate (EMS) is that segments 39 and 47, situated next to the secondary constrictions of standard chromosomes 6 and 7, when tandemly combined by reciprocal translocation in chromosome 76 of reconstructed karyotype T-21, behave in a rather similar way. This is independent of the nature of the chemical mutagens applied, as far as the distribution pattern of induced chromatid aberrations is concerned. The phenomenon may be characterized as follows: (1) the segments in question in the new position appeared to be the most pronounced aberration “hot spots”, the expressivity in segment 39 always being higher; (2) the pronounced “hot spot” character of these segments proved to be due mainly to their preferential involvement in intercalary deletions and duplication-deletions; (3) the specific constitution of chromosome 76 resulted in the majority of cases in a marked increase of the region involved in the aberration types mentioned above in segment 39. This is one of the very few examples of true position effect in the expression of chemically induced structural mutations at the chromosome level.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00226249
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  • 3
    Electronic Resource
    Electronic Resource
    Production and identification of new structural chromosome mutations in barley (Hordeum vulgare L.) (1996)
    Springer
    Theoretical and applied genetics 92 (1996), S. 777-781 
    Details
    ISSN: 1432-2242
    Keywords: Key words Reciprocal translocations ; Pericentric inversions ; Giemsa N-banding ; Hordeum vulgare
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  A total of 52 reciprocal translocations and 9 pericentric inversions were induced and identified in both standard and cytologically marked barley karyotypes using gamma-rays as the clastogenic agent. An analysis based upon Giemsa N-banding patterns and arm length measurements of the reconstructed chromosomes enabled a rather precise cytological localization of intra- and interchange breakpoints. This analysis was significantly facilitated and improved, especially for the identification of pericentric inversions, when the reconstructed karyotype T-1586 was used as starting material. The majority, if not all, of the aberration breakpoints proved to be localized in interband regions or in medial and terminal parts of the chromosomes, i.e., in regions which are deficient in constitutive heterochromatin. A great number of the structural mutations produced in this study contain specific cytological markers covering nearly all of the chromosomes of barley karyotype. This material might be of considerable interest in solving various problems of barley cytogenetics and chromosome engineering and especially in constructing a physical map of barley genome.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001220050191
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  • 4
    Electronic Resource
    Electronic Resource
    Position-specific effects in the mutagenic action of mitomycin C on the chromosomes of Hordeum vulgare L. (1989)
    Springer
    Theoretical and applied genetics 77 (1989), S. 705-710 
    Details
    ISSN: 1432-2242
    Keywords: Chromosome aberrations ; Translocation karyotypes ; Giemsa banding ; Mitomycin C ; Hordeum vulgare
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The mutagenic action of mitomycin C (MMC) on the chromosomes of two reconstructed karyotypes of barley was studied. MMC-induced chromatid aberrations were found to be distributed non-randomly along the chromosomes. The regions situated next to the secondary constrictions of chromosomes 6 and 7 appeared to be clearly pronounced aberration “hot spots”. In these segments, intercalary deletions and duplication-deletions were the most frequently induced aberration types. The comparative analysis of the frequency and localization of MMC-induced aberrations in the chromosomes of the two karyotype variants, which differ from each other by the position of the “hot spot” segments, provided new evidence about the influence of the segment transposition on the “hot spot” expressivity. The most remarkable finding obtained in the study is that the size of the segment involved in both intercalary deletions and duplication-deletions proved to be strongly affected by the structural peculiarity of the reconstructed chromosome. The possible reasons underlying this finding are discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00261248
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  • 5
    Electronic Resource
    Electronic Resource
    Intrachromosomal mapping of chromatid aberrations induced by restriction endonucleases in barley (1997)
    Springer
    Theoretical and applied genetics 94 (1997), S. 919-924 
    Details
    ISSN: 1432-2242
    Keywords: Key words Restriction endonucleases ; Chromatidaberrations ; Intrachromosomal distribution ; DNA ; Hordeum vulgare
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  The intrachromosomal distribution patterns of chromatid aberrations induced by the restriction endonucleases HpaII, MspI and HaeIII in a multireconstructed barley karyotype were analysed. All of these endonucleases, which differ with respect to their DNA recognition sequences but produce only one type of DNA damage, showed nearly the same pattern of localized chromosome breakage. The most pronounced aberration hot spots proved to be the loci of transcriptionally active and condensed, inactive, rDNA. Possible mechanisms involved in the specific distribution of induced aberrations along the distinct chromosomal regions are discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001220050496
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  • 6
    Electronic Resource
    Electronic Resource
    Production and identification of new structural chromosome mutations in barley (Hordeum vulgare L.) (1996)
    Springer
    Theoretical and applied genetics 92 (1996), S. 777-781 
    Details
    ISSN: 1432-2242
    Keywords: Reciprocal translocations ; Pericentric inversions ; Giemsa N-banding ; Hordeum vulgare
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A total of 52 reciprocal translocations and 9 pericentric inversions were induced and identified in both standard and cytologically marked barley karyotypes using gamma-rays as the clastogenic agent. An analysis based upon Giemsa N-banding patterns and arm length measurements of the reconstructed chromosomes enabled a rather precise cytological localization of intra- and interchange breakpoints. This analysis was significantly facilitated and improved, especially for the identification of pericentric inversions, when the reconstructed karyotype T-1586 was used as starting material. The majority, if not all, of the aberration breakpoints proved to be localized in interband regions or in medial and terminal parts of the chromosomes, i.e., in regions which are deficient in constitutive heterochromatin. A great number of the structural mutations produced in this study contain specific cytological markers covering nearly all of the chromosomes of barley karyotype. This material might be of considerable interest in solving various problems of barley cytogenetics and chromosome engineering and especially in constructing a physical map of barley genome.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00226101
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