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  • 1
    Electronic Resource
    Electronic Resource
    Scanning-beam electron microscopy of the conidia of the brown and albino filamentous varieties of Histoplasma capsulatum (1973)
    Springer
    Mycopathologia 49 (1973), S. 185-191 
    Details
    ISSN: 1573-0832
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Aspects of the surface appearance and external morphology of the conidial forms of the albino and brown filamentous varieties ofHistoplasma capsulatum as seen by scanning-beam electron microscopy are described and illustrated by electron micrographs. Septal areas between the hyphal cells of the supporting mycelium are seen as slightly elevated annular rings or ridges. The smooth micro- and macroconidia of the albino filamentous variety show a fine wrinkling and delicate irregularities of surface texture. Macroconidia of the brown filamentous variety are illustrated showing variations in numbers and respective length of the conspicuous wall projections or tubercles. The 3-dimensional perspective, unusual depth of focus, and high resolving power of the stereoscan technique permitted observations of external conidia morphology unattainable by other methods of study.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02050863
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  • 2
    Electronic Resource
    Electronic Resource
    Book reviews (1982)
    Springer
    Mycopathologia 79 (1982), S. 125-128 
    Details
    ISSN: 1573-0832
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00468090
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  • 3
    Electronic Resource
    Electronic Resource
    Aerobic respiratory activity of the yeastlike phase of Sporotrichum schenckii as affected by high hydrogen ion concentrations (1966)
    Springer
    Mycopathologia 28 (1966), S. 177-184 
    Details
    ISSN: 1573-0832
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Six strains ofSporotrichum schenckii were studied in regard to the effect of hydrogen ion concentration on certain aspects of the aerobic respiratory activity of the yeastlike phase. Optimal oxygen uptake of endogenous respiration occurred at pH 2.0, although no effects were observed on the oxygen-carbon dioxide exchange ratio (respiratory quotient) at this pH value when compared to the R.Q. obtained at pH 7.0. Endogenous respiratory activity at both pH 2 and 7 was markedly sensitive to the presence of certain respiratory inhibitors. Optimal respiratory activity using glucose as substrate occurred at pH 7.0 .On the other hand, oxidation of pyruvate as substrate proceeded at significant rates only at pH values below pH 4.0. With decreasing hydrogen ion concentration, accumulation of this organic acid occurred when glucose was employed as substrate. With the exception of acetate, none of the organic acid respiratory intermediates were found to stimulate respiration. The results reported herein suggest that the respiratory activity of the yeastlike phase ofS. schenckii differs in several respects from that observed for the yeastlike phases ofHistoplasma capsulatum andBlastomyces dermatitidis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02276049
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  • 4
    Electronic Resource
    Electronic Resource
    The possible role of uric acid in the ecology of Histoplasma capsulatum (1968)
    Springer
    Mycopathologia 35 (1968), S. 377-388 
    Details
    ISSN: 1573-0832
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The results of this study indicate thatHistoplasma capsulatum in its saprophytic form is able to utilize the major nitrogenous constituent of avian manure as a nitrogen source. In addition, the enzymes responsible for the pathway of uric acid degradation to inorganic nitrogen have been demonstrated in cell-free systems. These enzymes include uricase, allantoinase, allantoicase, and urease. The uricase ofHistoplasma appears to be a cell wall or cell membrane-associated enzyme, while the other enzymes were located in the soluble portion of cell-free extracts. Cell-free extracts ofCryptococcus neoformans are actively uricolytic. It is suggested that this ability ofH. capsulatum hyphae to utilize uric acid and related compounds as growth substrates may in part explain the indisputable ecologic association of this pathogenic fungus with avian and possibly chiropteran-associated soils and habitats in those areas endemic for histoplasmosis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02050753
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  • 5
    Electronic Resource
    Electronic Resource
    Electronmicroscopy of self-parasitism by Histoplasma capsulatum and Blastomyces dermatitidis (1970)
    Springer
    Mycopathologia 40 (1970), S. 271-276 
    Details
    ISSN: 1573-0832
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Zusammenfassung InHistoplasma capsulatum so wie auch inBlastomyces dermatitidis wurden in Hyphen und Zellen der Hefe- so wie auch der Mycelphase intracellular Hyphen durch Elektronmikrokopie nachgewiesen, wenn sie in flüssigen Schüttelkulturen gezüchtet worden sind. Diese Strukturen waren in Zellen der Hefephase, die zur Überleitung in die Myzelphase angeregt worden sind, sehr häufig. In vielen Fällen war eine sehr gute Bewahrung der ultrastrukturalen Einzelheiten der “parasitierten” Zelle, die es nahegelegt hat, dass die Zelle noch immer lebendig ist.
    Notes: Abstract Intrahyphal as well as intrayeast hyphae were demonstrated by electron-microscopy in bothHistoplasma capsulatum andBlastomyces dermatitidis yeastlike and mycelial phase cells grown in agitated liquid media. These structures appeared to be rather common in cultures of yeastlike cells induced to convert to the mycelial phase. In many cases there was excellent preservation of ultrastructural detail of the “parasitized” cell which suggested that the cell may still be viable.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02051780
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  • 6
    Electronic Resource
    Electronic Resource
    Yeastlike to mycelial phase transformation of Histoplasma capsulatum as observed by scanning electron microscopy (1971)
    Springer
    Mycopathologia 43 (1971), S. 185-193 
    Details
    ISSN: 1573-0832
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Details of the sequential events occurring during the critical phases of yeast to mold morphogenesis of the dimorphic fungal pathogenHistoplasma capsulatum as seen by the new technique of scanning electron microscopy are described and illustrated by electron micrographs. No conspicuous surface sculpturing was observed for the normal yeastlike cell immediately before or the newly formed hyphal cell after the critical period of transformation. However, both the parent yeastlike cell as well as the intermediate conversional cell shows a furrowing of the external cell surface which is due possibly to changes in internal cell pressure resulting from the migration of cell contents into the newly forming hyphal cell.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02051720
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  • 7
    Electronic Resource
    Electronic Resource
    Electron microscopy of the transitional conversion cell of Histoplasma capsulatum (1971)
    Springer
    Mycopathologia 44 (1971), S. 121-129 
    Details
    ISSN: 1573-0832
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Aspects of the fine structure of the transitional conversion cell formed during the early stages of the yeast to mold morphogenesis ofHistoplasma capsulatum as seen in ultrathin sections are described and illustrated by electron micrographs. Formation of the transitional cell was observed to occur with the highest degree of frequency between the 18th and 24th hr following induction of the conversional stimulus, although many yeastlike cells were observed to undergo degeneration or to initiate conversion only to abort the process. Cytoplasmic streaming and organelle migration from the parent yeast to the transitional cell was observed to occur prior to septation. The cell wall of the transitional form is thinner than that of the yeast and appears to arise from the inner portion of the laminated cell wall adjacent to the plasma membrane of the converting yeastlike cell. Interseptal or Woronin bodies were observed in association with the septal pore of the completed septum and were observed in the cytoplasm of both the yeastlike and transitional cell. The presence of these structures support strongly the pre-hyphal character of the converting cell complex.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02051880
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  • 8
    Electronic Resource
    Electronic Resource
    Role of the conidium in dimorphism of Blastomyces dermatitidis (1978)
    Springer
    Mycopathologia 64 (1978), S. 29-33 
    Details
    ISSN: 1573-0832
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Fine details of yeastlike cell development of Blastomyces dermatitidis from its conidium are described and illustrated by electron micrographs. When cultured in an enriched medium at 37 °C, conidia of two strains of B. dermatitidis readily underwent ultrastructural changes consistent with mycelial to yeast dimorphism. Although hyphal cells contained in the conversion cultures were observed consistently to undergo profound degenerative changes, the conidia rapidly germinated to give rise to short germ tubes which subsequently enlarged to form intermediate yeast mother cells (YMC). The wall of the germ tube arose from the innermost layer of the wall of the germinant. During the transition globoid osmiophilic inclusions of unknown origin and function were observed in vacuolated areas of the germ tube and YMC cytoplasm. Yeastlike daughter cells then budded from the intermediate YMC. Since transformation was readily accomplished under in vitro conditions favoring mycelial to yeast dimorphism, it is suggested that the conidium of B. dermatitidis represents the primary infective unit of this pathogenic fungus.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00443085
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  • 9
    Electronic Resource
    Electronic Resource
    Spontaneous feline sporotrichosis: A fine structural study (1979)
    Springer
    Mycopathologia 69 (1979), S. 57-62 
    Details
    ISSN: 1573-0832
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Fine structural details of the parasitic yeastlike phase of Sporothrix schenckii contained in biopsy tissue from a naturally-occurring case of disseminated feline sporotrichosis are described and illustrated by electron microscopy. Both free and phagocytosed fungal cells were observed. The fungal cells were contained within an extracellular, electron transparent vacuolar area which was bounded by a limiting membrane of probable host origin. The yeastlike cells were characterized by a conspicuous layer of osmiophilic microfilaments which occurred along the outermost surface of the cell wall. In many yeastlike cells, scattered, membranebound vacuoles containing electron opaque material were observed in the cytoplasm. Asteroid bodies were not observed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00428603
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  • 10
    Electronic Resource
    Electronic Resource
    The fine structure of the microconidium of blastomyces dermatitidis (1974)
    Springer
    Mycopathologia 52 (1974), S. 93-100 
    Details
    ISSN: 1573-0832
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Aspects of the fine structure of the microconidium of the mycelial phase of the dimorphic fungal pathogenBlastomyces dermatitidis as seen by techniques of scanning and transmission electron microscopy are described and illustrated by electron micrographs. The conidia ofB. dermatitidis undergo changes in the ultrastructural appearance of the cell wall as the spore matures. The cell wall becomes irregular in its thickness and possesses two distinct layers. No discrete or unique surface spines or projections were evident when the conidium ofB. dermatitidis was viewed by scanning electron microscopy. Upon maturity there is a marked deposition of lipid material in large, multiple storage bodies which occupy much of the cytoplasmic area. However, the cytoplasmic organelles appear to retain their structural integrity.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02128051
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