ISSN:
1435-1536
Keywords:
DNA
;
surfactant
;
complexes
;
toroidal conformation sedimentation
;
UV and CD spectroscopy
;
scanning tunneling and atomic force microscopy
Source:
Springer Online Journal Archives 1860-2000
Topics:
Chemistry and Pharmacology
,
Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
Notes:
Abstract A linear DNA can be dissolved in nonaqueous low-polar organic solvents by forming complexes between DNA and cationic surfactants. The data of high-rate sedimentation, UV-spectrometric and circular dichroism measurements show that the DNA complexes with surfactant in chloroform, heptane and cyclohexane are individual components with a 1∶1 stoichiometry. The DNA molecules passing to the organic phase retain a double-stranded helix conformation. By means of scanning tunneling and atomic force microscopy it was found that DNA-surfactant complexes in water and in chloroform exist in compact toroidal conformation. It was demonstrated that such complexes can be transferred through water/organic-phase boundaries.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF01189520
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