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  • 1
    Keywords: botany ; earthworms ; ectomycorrhiza ; drought ; heavy metal ; Infertile soils ; neighbour perception ; plasticity ; root systems ; root traits ; rhizobacteria ; rhizosphere ; deep roots
    Description / Table of Contents: Plant sciences research focuses predominantly on aboveground parameters. There is a scarcity of detailed information regarding the ecophysiology of root systems and the way root system functioning is affected by both internal and external factors. Furthermore, global climate change is expected to increase the intensity of climate extremes, such as severe drought, heat waves and periods of heavy rainfall; in addition other stresses such as salinization of soils are increasing world-wide. Recently an increasing awareness has developed that understanding plant traits will play a major role in breeding of future crop plants. For example, there is increasing evidence that the traits of root systems are defined by the properties of individual roots. However, further knowledge on the functional importance of root segments and the molecular/physiological mechanisms underlying root system functioning and persistence is needed, and would specifically allow modifying (crop) root system functionality and efficiency in the future. Another major gap in knowledge is localized at the root-soil interface and in regard to the potential adaptive plasticity of root-rhizosphere interactions under abiotic stress and/or competition. It is currently unknown whether adaptations in microbe communities occur, for example due to modified exudation rates, and what are the subsequent influences on nutrient mobilization and uptake. Furthermore, uncovering the mechanisms by which roots perceive neighboring roots may not only contribute to our understanding of plant developmental strategies, but also has important implications on the study of competitive interactions in natural communities, and in optimizing plant performance and resource use in agricultural and silvicultural systems. In this research topic, we aim to provide an on-line, open-access snapshot of the current state of the art of the field of root ecology and physiology, with special foci on the translation of root structure to function, and how root systems are influenced by interplay with internal and external factors such as abiotic stress, microbes and plant-plant interaction. We warmly welcome original research papers, but reviews of specific topics, articles formulating opinions or describing cutting-edge methods are also gladly accepted.
    Pages: Online-Ressource (316 Seiten)
    ISBN: 9782889192854
    Language: English
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  • 2
    ISSN: 1432-1890
    Keywords: Calcium ; Iron ; Lead ; Phosphorus ; Short roots ; X-ray microanalysis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Norway spruce [Picea abies (L.) Karst.] seedlings, nonmycorrhizal of mycorrhizal with Laccaria laccata or Paxillus involutus were grown in a quartz sand-nutrient solution system for 6 months and then treated with 5 μM Pb for 4 days. Element contents of cortex cell wall of young, medium and old short roots were determined by X-ray microanalysis of longitudinal thin sections. The Pb content was influenced neither by age nor by the distance from the root tip (up to 1.7 mm) but was significantly lower in the P. involutus mycorrhizae than in the L. laccata mycorrhizae or in nonmycorrhizal short roots. In the P. involutus mycorrhizae, the P content of the cortex cell walls was twice as high in young mycorrhizae than in old mycorrhizae. In the nonmycorrhizal short roots and the L. laccata mycorrhizae, P content was influenced neither by age nor by distance from the root tip. The Ca and Fe contents of the cortex cell walls increased with age in the nonmycorrhizal short roots and the mycorrhizae. It is concluded that the element content of the cortex cell walls of short roots is strongly influenced by age, while the distance from the root tip seems to be of minor importance.
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 1432-1890
    Keywords: Key words Calcium ; Iron ; Lead ; Phosphorus ; Short roots ; X-ray microanalysis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  Norway spruce [Picea abies (L.) Karst.] seedlings, nonmycorrhizal of mycorrhizal with Laccaria laccata or Paxillus involutus were grown in a quartz sand-nutrient solution system for 6 months and then treated with 5 μM Pb for 4 days. Element contents of cortex cell wall of young, medium and old short roots were determined by X-ray microanalysis of longitudinal thin sections. The Pb content was influenced neither by age nor by the distance from the root tip (up to 1.7 mm) but was significantly lower in the P. involutus mycorrhizae than in the L. laccata mycorrhizae or in nonmycorrhizal short roots. In the P. involutus mycorrhizae, the P content of the cortex cell walls was twice as high in young mycorrhizae than in old mycorrhizae. In the nonmycorrhizal short roots and the L. laccata mycorrhizae, P content was influenced neither by age nor by distance from the root tip. The Ca and Fe contents of the cortex cell walls increased with age in the nonmycorrhizal short roots and the mycorrhizae. It is concluded that the element content of the cortex cell walls of short roots is strongly influenced by age, while the distance from the root tip seems to be of minor importance.
    Type of Medium: Electronic Resource
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  • 4
    ISSN: 1432-0789
    Keywords: Ectomycorrhiza ; Lactarius ; Minearl nutrition ; Picea abies ; Plant growth ; Protozoa ; Rhizosphere
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Mycorrhizal (Lactarius rufus Fr.) and non-mycorrhizal Norway spruce seedlings (Picea abies Karst.) were grown in a sand culture and inoculated with protozoa (naked amoebae and flagellates) extracted from native forest soil or with protozoa grown on agar cultures. A soil suspension from which the protozoa were eliminated by filtration or chloroform fumigation was used as a control. After 19 weeks of growth in a climate chamber at 20–22°C, the seedlings were harvested. Protozoa reduced the number of bacterial colony-forming units extracted from the rhizoplane of both non-mycorrhizal and mycorrhizal seedlings and significantly increased seedling growth. However, concentrations of mineral nutrients in needles were not increased in seedlings with protozoan treatment. It is concluded that the increased growth of seedling was not caused by nutrients released during amoebal grazing on rhizosphere micro-organisms. The protozoa presumably affected plant physiological processes, either directly, via production of phytohormones, or indirectly, via modification of the structure and performance of the rhizosphere microflora and their impact on plant growth. Mycorrhizal colonization significantly increased the abundance of naked amoebae at the rhizoplane. Our observations indicate that protozoa in the rhizosphere interact significantly with mycorrhizae.
    Type of Medium: Electronic Resource
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  • 5
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Physiologia plantarum 82 (1991), S. 0 
    ISSN: 1399-3054
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Spruce seedlings (Picea abies (L.) Karst.) colonized with Lactarius rufus (Scop.) Fr. or Lactarius theiogalus Fr. were grown in an axenic silica sand culture system with frequently renewed nutrient solution. After successful mycorrhizal colonization the seedlings were exposed to 800 μM Al(NO3)3 (pH 3.9) for 13 or 17 weeks. Concentrations of Al, Mg, and Ca in the tissues of the mycorrhizal root tips were determined by X-ray microanalysis. After 13 or 17 weeks of exposure to Al, high Al concentrations were found in cell walls of all mycorrhizal tissues except the stele tissues. Compared to the controls Mg levels in most of the mycorrhizal structures were reduced by Al treatment. Calcium levels in cortex cell walls of root tips colonized with L. rufus were reduced by exposure to Al. However, in cell walls of the stele Ca levels were significantly increased. No differences in Al or Mg levels were detected in structures of mycorrhizal and non-mycorrhizal root tips from the same individual seedlings. These results suggest that (1) the endodermis is the primary barrier to radial Al transport and (2) the presence of a hyphal sheath did not prevent Al from reaching the root cortex and from displacing Mg and Ca.
    Type of Medium: Electronic Resource
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  • 6
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Physiologia plantarum 81 (1991), S. 0 
    ISSN: 1399-3054
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: A sand culture system was developed for growth of mycorrhizal seedlings under monoxenic conditions, with frequently renewed nutrient solution The composition of the nutrient solution resembled that of a forest soil solution, based on long-term measurements from forest sites at Soiling, northern Germany. Seedlings of Picea abies (L.) Karst. inoculated with Lactarius rufus (Scop.) Fr. were grown in this culture system. Plants developed rapidly, having almost totally mycorrhizal root systems. Nitrate at 2.7 mM in the nutrient solution and applied over a 13 week period had no negative effect on mycorrhizal development. Ammonium at 2.7 mM reduced the degree of mycorrhizal infection slightly, in such a way that the degree of mycorrhizal infection was reduced to a much less extent than the total number of root lips. Hence, the impact of NH+4 may be primarily on root development and not on mycorrhizal fungal colonization. When the concentrations of NO-3 and NH+4 used in the present study are compared to those found in forest soil solutions, NO-3 and NH4+ would not appear to influence mycorrhizal development negatively under natural conditions. Aluminium at 0.8 mM and applied over a 13 week period reduced Mg uptake into roots and needles by 52 and 64%, respectively, resulting in needle chlorosis and strongly reduced photosynthetic activity. From a comparison of this study with others, no major difference in physiological response to aluminium exposure between non-mycorrhizal seedlings and seedlings colonized with Lactarius rufus was found.
    Type of Medium: Electronic Resource
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  • 7
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Science Ltd
    Global change biology 9 (2003), S. 0 
    ISSN: 1365-2486
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Energy, Environment Protection, Nuclear Power Engineering , Geography
    Notes: A fast growing high density Populus plantation located in central Italy was exposed to elevated carbon dioxide for a period of three years. An elevated CO2 treatment (550 ppm), of 200 ppm over ambient (350 ppm) was provided using a FACE technique. Standing root biomass, fine root turnover and mycorrhizal colonization of the following Populus species was examined: Populus alba L., Populus nigra L., Populus x euramericana Dode (Guinier). Elevated CO2 increased belowground allocation of biomass in all three species examined, standing root biomass increased by 47–76% as a result of FACE treatment. Similarly, fine root biomass present in the soil increased by 35–84%. The FACE treatment resulted in 55% faster fine root turnover in P. alba and a 27% increase in turnover of roots of P. nigra and P. x euramericana. P. alba and P. nigra invested more root biomass into deeper soil horizon under elevated CO2. Response of the mycorrhizal community to elevated CO2 was more varied, the rate of infection increased only in P. alba for both ectomycorrhizal (EM) and arbuscular mycorrhizas (AM). The roots of P. nigra showed greater infection only by AM and the colonization of the root system of P. x euramericana was not affected by FACE treatment. The results suggest that elevated atmospheric CO2 conditions induce greater belowground biomass investment, which could lead to accumulation of assimilated C in the soil profile. This may have implications for C sequestration and must be taken into account when considering long-term C storage in the soil.
    Type of Medium: Electronic Resource
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  • 8
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Physiologia plantarum 97 (1996), S. 0 
    ISSN: 1399-3054
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Copper and cadmium uptake and distribution in 2-week-old seedlings of stone pine (Pinus pinea L.), maritime pine (Pinus pinaster Ait.) and ash (Fraxinus angustifolia Vahl.) were investigated. Seedlings were grown in culture solution at increasing concentrations of CuSO4 (0.012–5 μM) and CdSO4 (0.0–5 μM). All species accumulated copper in a larger amount than cadmium. Translocation to the shoot was strongly restricted for both metals, though higher cadmium mobility within the plant could be evidenced. A strong relationship between root content and growth inhibition was detected in the roots in response to copper but not to cadmium treatments. Among species P pinea seemed to be more tolerant to cadmium, whereas F. angustifolia was highly sensitive to both cadmium and copper. X–ray microanalysis of root tip sections showed that copper and cadmium only accumulated in the root tips of F. angustifolia. copper mostly in the cell walls of the cortex layer, cadmium in the inner compartments of the cortex cells. It is suggested that in the two Pinus species the well developed root cap plays a protecting role against metal uptake at the root–tip zone.
    Type of Medium: Electronic Resource
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  • 9
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Physiologia plantarum 92 (1994), S. 0 
    ISSN: 1399-3054
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Heavy metal loads in forest soils have been increasing over time due to atmospheric inputs. Accumulation in the upper soil layers could affect establishment of seedlings and forest regeneration. Mediterranean species show a high initial root development, allowing seedlings to reach the moisture of deeper soil layers. In the present work seedlings of stone pine (Pinus pinea L.) and maritime pine (Pinus pinaster Ait.), were grown in culture solution supplied with 0.0, 0.1, 1 or 5 μM CdSO4 or with 1 μM CdSO4 and 1 μM CuSO4 combined. In both species tap-root elongation was drastically reduced in the 5 μM Cd2+ and in the (Cd2++ Cu2+) treatments. A supply of 0.1 or 1 μM Cd2+, however, enhanced root elongation in Pinus pinea without significantly influencing root elongation in Pinus pinaster. In both species the root density (weight per unit length) and the width of the cortex increased in response to Cd2+ exposure. In Pinus pinaster the mitotic index decreased at the higher Cd2+ concentrations and when Cd2+ and Cu2+ were combined. The data suggest that cell elongation is more sensitive to Cd2+ than cell division. The number and length of the lateral roots were also affected by Cd2+ treatment to a higher degree in Pinus pinaster than in Pinus pinea, reflecting the different Cd- tolerance of the two species.
    Type of Medium: Electronic Resource
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  • 10
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Physiologia plantarum 81 (1991), S. 0 
    ISSN: 1399-3054
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Non-mycorrhizal spruce seedlings(Picea abies Karst.) and spruce seedlings colonized with Lactarius rufus (Scop.) Fr. or two strains of Paxillits involutus (Batsch) Fr. were grown in an axenic silica sand culture system with frequently renewed nutrient solution. After successful mycorrhizal colonization, the seedlings were exposed to 1 μM PbCI2 for 19 weeks. The degree of infection in all of the mycorrhizal treatments approached 100% during the experiment and was not affected by exposure to Pb. However, the number of root tips per root dry weight and the shoot: root ratio, both in the non-mycorrhizal and the mycorrhizal seedlings, had decreased after the 19 week treatment with PbCl2 Using X-ray microanalysis, the distribution and concentration of Pb in the tissues of mycorrhizal and non-mycorrhizal root tips were compared. In the mycorrhizae of seedlings exposed to Pb no significant accumulation of Pb in the hyphal mantle or in fungal cell walls of the Hartig net were detected. Lead accumulated primarily in the cortex cell walls both of non-mycorrhizal and mycorrhizal root tips. No significant difference of Pb concentrations in root cortex cell walls of non-mycorrhizal and mycorrhizal seedlings was found; except for seedlings colonized with Paxillus involutus strain 537. However, at the endodermis no effect of mycorrhizal fungal colonization on the Pb tissue concentration was detected. The presence of the fungal sheath did not prevent Pb from reaching the root cortex. The endodermis acted as a barrier to Pb radial transport in both mycorrhizal and non-mycorrhizal seedling roots.
    Type of Medium: Electronic Resource
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