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  • 1
    Electronic Resource
    Electronic Resource
    Altered growth of Fusarium oxysporum f. sp. chrysanthemi in an in vitro dual culture system with the vesicular arbuscular mycorrhizal fungus Glomus intraradices growing on Daucus carota transformed roots (1995)
    Springer
    Mycorrhiza 5 (1995), S. 431-438 
    Details
    ISSN: 1432-1890
    Keywords: Pathogen ; Biological control ; Spore ; Conidia ; Germination ; VAM
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Vesicular arbuscular mycorrhizal fungi can reduce plant disease symptoms and populations of pathogens through mechanisms that are not well understood. Glomus intraradices was grown on Daucus carota transformed roots in a two-compartment in vitro system. One compartment contained mycorrhizal roots on a complete growth medium, while the other contained a medium lacking sugar on which only mycelial growth was allowed. The direct interaction between G. intraradices and Fusarium oxysporum f. sp. chrysanthemi was studied in the compartment lacking sugar during a 5-day period. G. intraradices hyphal density and spore number were estimated along with F. o. chrysanthemi conidial germination, mycelial growth and sporulation. Five hours after inoculation, germination of F. o. chrysanthemi conidia doubled in the presence of G. intraradices. Radial growth of F. o. chrysanthemi colonies was always slightly but significantly enhanced in the presence of G. intraradices. No correlation was obtained between G. intraradices hyphae or spore densities and F. o. chrysanthemi hyphal growth. Overall sporulation of the 5-day-old F. o. chrysanthemi colonies was not influenced by the presence of G. intraradices. However, significant negative correlations were found between F. o. chrysanthemi conidia production and G. intraradices hyphae or spore concentrations. G. intraradices increased F. o. chrysanthemi conidial germination and slightly stimulated its hyphal growth in dual culture without any root influences. No antibiosis was observed between the fungi. The significance of the results and their potential implication for rhizosphere biology are discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00213444
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  • 2
    Electronic Resource
    Electronic Resource
    Colonization potential of in vitro-produced arbuscular mycorrhizal fungus spores compared with a root-segment inoculum from open pot culture (1999)
    Springer
    Mycorrhiza 8 (1999), S. 335-338 
    Details
    ISSN: 1432-1890
    Keywords: Key words Inoculum ; Co-culture ; Spore ; Soil ; Glomales ; AM
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  A reliable inoculum, free from other microorganisms, to produce arbuscular mycorhizal (AM) plants is of the greatest importance when studying the interaction between AM plants and soil microorganisms. We investigated the colonization of leeks from monoxenic in vitro-produced Glomus intraradices spores. The isolated spores were produced using a two-compartment in vitro growth system previously described. A spore suspension was used as inoculum and was compared to the inoculum potential of endomycorrhizal root segments of pot-grown leek (Allium porrum L.) plants. The leeks were grown in a controlled environment and two types of sterilized growth media were tested: calcined montmorillonite clay and a soil mix. Root colonization progressed faster in the soil mix than in the clay. However, in this medium, after an initial delay, root colonization from in vitro-produced spores was essentially the same as that observed with the root-segment inoculum, reaching 44% and 58% respectively, after 16 weeks. Leek roots colonized by the monoxenically-produced spores harbored only the studied AMF fungi while the roots colonized from the root segments were substantially contaminated by other fungi.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s005720050255
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  • 3
    Electronic Resource
    Electronic Resource
    Effects of N source on pH and nutrient exchange of extramatrical mycelium in a mycorrhizal Ri T-DNA transformed root system (1996)
    Springer
    Mycorrhiza 6 (1996), S. 247-251 
    Details
    ISSN: 1432-1890
    Keywords: Key words Glomus intraradices ; Nitrogen source ; Extramatrical mycelium ; Mycorrhizal transformed roots ; pH
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  The influence of different N sources on medium pH variation and the effect of the external mycelium of arbuscular mycorrhizal fungi on nutrient dynamics were studied using a two-compartment, aseptic Petri plate system. VA mycorrhizal, transformed roots of carrot (Daucus carota L.) were cultured in the proximal compartment and external mycorrhizal mycelium in the distal compartment. The medium in the distal compartament contained N either as NO3 – or as NH4 +. The pH and the anion and cation concentrations were measured every 15 days in filtrates prepared from the distal compartments. Thirteen weeks after colonization, there was a significant basification or a light acidification of the NO3 – and NH4 + medium, respectively. There was no change in NO3 – concentration but a significant decrease in NH4 + concentration. Treatments containing N as NO3 – showed no variation in cations such asCa2+ and Mg2+ or anions such as PO4 2–, and SO4 2– but showed significant increases in the concentration of K+. Treatments containing N as NH4 + showed no variation in cations or anions, except for increases in the concentrations of K+ and Cl–.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s005720050132
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  • 4
    Electronic Resource
    Electronic Resource
    Altered growth of Fusarium oxysporum f.sp. chrysanthemi in an in vitro dual culture system with the vesicular arbuscular mycorrhizal fungus Glomus intraradices growing on Daucus carota transformed roots (1995)
    Springer
    Mycorrhiza 5 (1995), S. 431-438 
    Details
    ISSN: 1432-1890
    Keywords: Key words Pathogen ; Biological control ; Spore ; Conidia ; Germination ; VAM
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  Vesicular arbuscular mycorrhizal fungi can reduce plant disease symptoms and populations of pathogens through mechanisms that are not well understood. Glomus intraradices was grown on Daucus carota transformed roots in a two-compartment in vitro system. One compartment contained mycorrhizal roots on a complete growth medium, while the other contained a medium lacking sugar on which only mycelial growth was allowed. The direct interaction between G. intr aradices and Fusarium oxysporum f. sp. chrysanthemi was studied in the compartment lacking sugar during a 5-day period. G. intraradices hyphal density and spore number were estimated along with F. o. chrysanthemi conidial germination, mycelial growth and sporulation. Five hours after inoculation, germination of F. o. chrysanthemi conidia doubled in the presence of G. intraradices. Radial growth of F. o. chrysanthemi colonies was always slightly but significantly enhanced in the presence of G. intraradices. No correlation was obtained between G. intraradices hyphae or spore densities and F. o. chrysanthemi hyphal growth. Overall sporulation of the 5-day-old F. o. chrysanthemi colonies was not influenced by the presence of G. intraradices. However, significant negative correlations were found between F. o. chrysanthemi conidia production and G. intraradices hyphae or spore concentrations. G. intraradic es increased F. o. chrysanthemi conidial germination and slightly stimulated its hyphal growth in dual culture without any root influences. No antibiosis was observed between the fungi. The significance of the results and their potential implication for rhizosphere biology are discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00213444
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  • 5
    Electronic Resource
    Electronic Resource
    Rapid Prediction of Maple Syrup Grade and Sensory Quality by Estimation of Microbial Quality of Maple Sap Using ATP Bioluminescence (2002)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of food science 67 (2002), S. 0 
    Details
    ISSN: 1750-3841
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: : ATP bioluminescence was evaluated as a method for assessing the level of microbial contamination in sap and predicting maple syrup characteristics. This approach provided results that were strongly correlated with the standard plate count and took less time than the modified resazurin technique. ATP bioluminescence measurement of sap proved to be reliable for predicting physicochemical and sensory characteristics as indicated by the color and flavor of maple syrup. Most of the syrups made from saps with higher ATP bioluminescence values were darker in color and presented off-flavors. Based on these results, ATP bioluminescence could be used to improve sanitary practices associated with collecting and storing maple sap.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-2621.2002.tb08734.x
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  • 6
    Electronic Resource
    Electronic Resource
    Differential Diagnosis between Learning-disabled and Dysphasic Children (1993)
    Oxford, UK : Blackwell Publishing Ltd
    Annals of the New York Academy of Sciences 682 (1993), S. 0 
    Details
    ISSN: 1749-6632
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Natural Sciences in General
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1749-6632.1993.tb22990.x
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  • 7
    Electronic Resource
    Electronic Resource
    Chemiluminescent and Bioluminescent Reporter Gene Assays
    Amsterdam : Elsevier
    Analytical Biochemistry 219 (1994), S. 169-181 
    Details
    ISSN: 0003-2697
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology , Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1006/abio.1994.1254
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  • 8
    Electronic Resource
    Electronic Resource
    Synthesis of macromolecules in epithelial cells of the cultured amphibian lens - I. DNA and RNA
    Amsterdam : Elsevier
    Experimental Cell Research 44 (1966), S. 303-311 
    Details
    ISSN: 0014-4827
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology , Medicine
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1016/0014-4827(66)90436-8
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  • 9
    Electronic Resource
    Electronic Resource
    Interaction entre Basidiomycètes mycorrhizateurs et racines de pin en presence d'acide indol-3yl-acetique (1970)
    Oxford, UK : Blackwell Publishing Ltd
    Physiologia plantarum 23 (1970), S. 0 
    Details
    ISSN: 1399-3054
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Interaction between mycorrhizal Basidiomycetes and pine roots in presence of indol-3yl-acetic acid. — La croissance de deux Basidomycètes mycorrhizateurs, Suillus variegatus (Sw. ex Fr.) et S. granulatus (L. ex Fr.) O. Kuntze a été rendue possible dans un milieu contenant au départ des quantités d'acide indol-3yl-acétique (AIA) inhibitrices pour ces champignons. Pour obtenir ce résultat, il a suffi d'ajouter au miliceu, des racines isolées et vivantes de pin (Pinus sylvestris L. et P. resinosa Ait.).Plus la quantité d'AIA ajoutée au départ était grande, plus il fallait de racines pour renverser l'inhibition. L'exsudat des racines n'a produit aucun effet comparable.Des mesures effectuées sur une solution d'IAI ont montré que les racines isolées de pin peuvent accumuler rapidement cette substance. Le taux d'accumulation est maximum aux valeurs de pH inférieures à 4.0 et devient très faible au voisinage de la neutralité.L'auteur formule l'hypothèse qu'au cours de la formation des mycorrhizes ectotrophes chez le pin, la racine en accumulant l'AIA favorise la croissance du champignon en prévenant l'auto-inhibition de son mycélium par l'AIA qu'il produit.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1399-3054.1970.tb06426.x
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  • 10
    Electronic Resource
    Electronic Resource
    Action inhibitrice de l'acide 3-indolyl-acétique sur la croissance de quelques Basidiomycètes mycorhizateurs (1967)
    Oxford, UK : Blackwell Publishing Ltd
    Physiologia plantarum 20 (1967), S. 0 
    Details
    ISSN: 1399-3054
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: La croissance en milieu liquide de quelques champignons mycorhizateurs a étéétudiée en présence d'acide 3-indlolyl-acétique (AIA). Les champignons suivants ont été utilisés: Amanita muscaria (L. ex Fr.) Quél., A. rubscens (Pers. ex Fr.) Quél., Suillus granulatus (L. ex Fr.) O. Kuntze et S. variegatus (Sw. ex Fr.) O. Kuntze. Tous ont été inhibés, à des degrés divers, par l'AlA à la concentration do 1.75 mg/l.Soumis à diverses concentrations d'AIA, S. variegatus a montré une croissancc légèrement accrue au voisinage de 0.2 mg/l de cette substance. Cependant, pour des concentrations supérieures à celle-ci, l'inhibition de la croissancc a été proportion-nelle à la concentration en AIA; elle a été de 50 pour-cent en présence de 2.0 mg/l d'AIA.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1399-3054.1967.tb07193.x
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