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  • 1
    Electronic Resource
    Electronic Resource
    Optimization and comparison of MAE, ASE and Soxhlet extraction for the determination of HCH isomers in soil samples (2000)
    Springer
    Fresenius' journal of analytical chemistry 367 (2000), S. 733-737 
    Details
    ISSN: 1432-1130
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract The microwave-assisted extraction (MAE), accelerated solvent extraction (ASE) and Soxhlet extraction of two isomers of hexachlorocyclohexane, α-HCH and γ-HCH, from a polluted landfill soil have been optimized following different experimental designs. In the case of microwave-assisted extraction, the following variables were considered: pressure, extraction time, microwave power, percentage of acetone in n-hexane mixture and solvent volume. When ASE extraction was studied the variables were pressure, temperature and extraction time. Finally, the percentage of acetone in n-hexane mixture and the extraction time were the only variables studied for Soxhlet extraction. The concentrations obtained by the three extraction techniques were, within their experimental uncertainties, in good agreement. This fact assures the possibility of using both ASE and MAE techniques in the routine determination of lindane in polluted soils and sediments.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002160000459
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  • 2
    Electronic Resource
    Electronic Resource
    Assessing the organizational climate and contractual relationship for perceptions of support for innovation (2004)
    Bingley : Emerald
    International journal of manpower 25 (2004), S. 167-180 
    Details
    ISSN: 0143-7720
    Source: Emerald Fulltext Archive Database 1994-2005
    Topics: Economics
    Notes: The aim of this paper is to study the relationship between organizational climate and perceptions of support for innovation, considering that the relationship may be moderated by the type of labor contract the employees have. This moderating effect may have its origins in the perception of reality and the type of knowledge applied on the job. The results drawn from empirical research among 312 observations of the employees in 80 offices of a Spanish financial company enable us to verify, on the one hand, that an organizational climate characterized by support, cohesion and intrinsic recognition favors perceptions of support for innovation; and, on the other hand, that there are differences in the dimensions of climate that favor perceptions of support for innovation depending on the employees' contractual relationship with the organization.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1108/01437720410535972
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  • 3
    Electronic Resource
    Electronic Resource
    Determinants of innovation through a knowledge-based theory lens (2002)
    Bingley : Emerald
    Industrial management & data systems 102 (2002), S. 289-296 
    Details
    ISSN: 0263-5577
    Source: Emerald Fulltext Archive Database 1994-2005
    Topics: Computer Science , Economics
    Notes: In this paper, a model for determining innovation degree in service industries is presented. Such a model is developed under the knowledge-based theory lens. So, knowledge flows and knowledge integration capabilities of the organisation's members are considered as crucial for the innovation processes to be successfully implemented. The model is tested in a sample of engineering consulting firms of Spain. Main results point out the strong explanatory power of innovation intensity with knowledge theory-based models. Final conclusions consider knowledge management policies as the main impellers of service innovation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1108/02635570210428320
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  • 4
    Electronic Resource
    Electronic Resource
    Factors affecting the relationship between total quality management and organizational performance (2003)
    Bingley : Emerald
    International journal of quality & reliability management 20 (2003), S. 189-209 
    Details
    ISSN: 0265-671X
    Source: Emerald Fulltext Archive Database 1994-2005
    Topics: Economics
    Notes: This paper aims to provide a framework for studying the relationship between total quality management (TQM) and organizational performance. TQM contents as well as TQM elements are considered. So, from a contingency approach, TQM contents have to be consistent with business orientation and environmental uncertainty in order to be effective. On the other hand, the relationship between TQM elements and performance is developed from an industrial psychology perspective. Hence, TQM elements are considered to impact both behavioural and individuals' learning processes. In the proposed model this relations are mediated by the TQM-driven cultural change acceptance. Moreover, TQM elements impact these individual processes both directly and mediated by systems and personal factors. Hence, both TQM contents and elements have to be considered to do the right things and to do it well.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1108/02656710310456617
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  • 5
    Electronic Resource
    Electronic Resource
    Mediation of sterol-induced calmodulin synthesis in Phaseolus vulgaris roots by Ca2+ and its possible relationship to plant growth regulators (1989)
    Oxford, UK : Blackwell Publishing Ltd
    Physiologia plantarum 75 (1989), S. 0 
    Details
    ISSN: 1399-3054
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Vitamin D3 and stigmasterol have been previously shown to stimulate growth, Ca2+ fluxes and calmodulin synthesis in Phaseolus vulgaris roots. In this study, these sterols (10−9M) were shown to accelerate the incorporation of [3H]-thymidine into DNA in Phaseolus vulgaris (L. cv. Contraancha) root apices, similarly to a mixture of the mitogenic plant growth factors 2,4-dichlorophenoxyacetic acid and kinetin (4.6 μM each). The effects of stigmasterol were blocked by flufenazine, a calmodulin antagonist. Analogously to stigmasterol, the plant hormones stimulated calmodulin synthesis as shown by double labeling of root proteins with [14C]-leucine and [3H]-leucine, respectively, followed by their separation on sodium dodecyl sulfate-po-lyacrylamide gels and a calmodulin affinity column, immunoblot analysis and cyclic AMP phosphodiesterase activation assays. The stimulation of root calmodulin formation by stigmasterol was abolished in the absence of Ca2+ in the incubation medium and was mimicked by the Ca2+ ionophore A–23187. The results suggest that the sterols, like plant mitogenic hormones, promote DNA synthesis, and that these compounds stimulate calmodulin synthesis as a consequence of their mitogenic activity. Ca2+ appears to mediate the action of the sterols.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1399-3054.1989.tb05615.x
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  • 6
    Electronic Resource
    Electronic Resource
    Exploring prokaryotic diversity: there are other molecular worlds (2005)
    Oxford, UK : Blackwell Publishing Ltd.
    Molecular microbiology 55 (2005), S. 0 
    Details
    ISSN: 1365-2958
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Medicine
    Notes: Prokaryotes are the major source of biological diversity on earth. This is not simply because of the large number of species present, or because of their diverse growth conditions and environmental niches populated by them, but because of the wealth of genes, metabolic pathways and molecular processes that are only found in prokaryotic cells. Therefore, Bacteria and Archaea (and their phages) cannot be considered any longer as miniaturized models of Eukaryotes, but as a genuine source of unique biological processes that are mediated by unique sets of genes and molecular devices. A true understanding of complex biological phenomena will require a deeper knowledge of this vast prokaryotic world. The second European Molecular Biology Organization (EMBO) conference on Molecular Microbiology entitled ‘Exploring Prokaryotic Diversity’ explored many aspects of this newly emerging interest in the prokaryotic world.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-2958.2004.04381.x
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  • 7
    Electronic Resource
    Electronic Resource
    Sulfur-selective desulfurization of dibenzothiophene and diesel oil by newly isolated Rhodococcus sp. strains (2002)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 215 (2002), S. 0 
    Details
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: New desulfurizing bacteria able to convert dibenzothiophene into 2-hydroxybiphenyl and sulfate were isolated from contaminated soils collected in Mexican refineries. Random amplified polymorphic DNA analysis showed they were different from previously reported Rhodococcus erythropolis desulfurizing strains. According to 16S rRNA gene sequencing and fatty acid analyses, these new isolates belonged to the genus Rhodococcus. These strains could desulfurize 4,6-dimethyldibenzothiophene which is one of the most difficult dibenzothiophene derivatives to remove by hydrodesulfurization. A deeply hydrodesulfurized diesel oil containing significant amounts of 4,6-dimethyldibenzothiophene was treated with Rhodococcus sp. IMP-S02 cells. Up to 60% of the total sulfur was removed and all the 4,6-dimethyldibenzothiophene disappeared as a result of this treatment.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1574-6968.2002.tb11385.x
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  • 8
    Electronic Resource
    Electronic Resource
    Observations of shear-wave polarizations from rockbursts in a South African gold field: an analysis of acceleration and velocity recordings (1991)
    Oxford, UK : Blackwell Publishing Ltd
    Geophysical journal international 107 (1991), S. 0 
    Details
    ISSN: 1365-246X
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Geosciences
    Notes: Rockbursts in the deep gold mines of South Africa have seismic magnitudes ranging from ML less than zero to ML more than 5. They occur in very confined volumes surrounding the working face of the mining excavations. An examination of three-component acceleration and velocity seismograms shows that the polarizations of shear waves recorded within the shear-wave window above an active mining area have a nearly uniform alignment. The polarization alignment and the measured time delays are consistent with shear waves propagating through the effective anisotropy of parallel vertical microcracks throughout the rockmass. Polarizations measured from velocity transducers were compared with polarizations measured from strong-motion acceleration recordings to show the amount of scatter in the data. We conclude that the dry fractures caused by the high stresses during normal mining processes have negligible effect at the wavelengths at which shear waves are recorded at the surface. The anisotropy observed at the surface appears to be due to microcracks aligned by the regional stress regime rather than local disturbances to the stress regime due to mining operations.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-246X.1991.tb01425.x
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  • 9
    Electronic Resource
    Electronic Resource
    Secretion and assembly of regular surface structures in Gram-negative bacteria (2000)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology reviews 24 (2000), S. 0 
    Details
    ISSN: 1574-6976
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Bacteria synthesize large-sized surface structures through the ordered polymerization of protein subunits. This results in planar or tubular regular structures that have evolved to accomplish specific functions related to the particular environment in which these bacteria are found. Tubular assemblies known as flagella are the most complex structures known in bacteria and consist of a helical rigid filament, a torsion adapter or hook and a proton-fueled rotator known as the basal body. Pili or fimbriae are less complicated helical filaments, which consist of a major subunit and 3–5 minor subunits or pilins, whose main function is the attachment to specific surfaces. Planar structures known as S-layers are the simplest of these regular assemblies and are generally made up of a single subunit packed as a bidimensional crystal around the whole cell surface. Most of the components of these structures have to be secreted through the inner membrane (IM), the periplasm and the outer membrane (OM) before reaching their final destination. The so called general secretory pathway (GSP), or type II secretion system, appears to be implicated in this process to varying degrees, depending on the structure considered. A few S-layers and pili require GSP components but also need specific terminal branches, such as the well known chaperone-usher pathway. On the other hand, only two of the nearly 40 proteins involved in flagellar assembly are dependent on the GSP, while the external components are secreted through a specific pathway similar to the type III systems identified in some pathogens. Moreover, secretion of subunits of S-layers using dedicated type I machinery, without the involvement of any GSP component, has also been observed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1574-6976.2000.tb00531.x
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  • 10
    Electronic Resource
    Electronic Resource
    Structural tolerance of bacterial autotransporters for folded passenger protein domains (2004)
    Oxford, UK : Blackwell Science Ltd
    Molecular microbiology 52 (2004), S. 0 
    Details
    ISSN: 1365-2958
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Medicine
    Notes: In this report we investigate the capacity of bacterial autotransporters (AT) to translocate folded protein domains across the outer membrane (OM). Polypeptides belonging to the AT family contain a C-terminal domain that supports the secretion of the N-domain (the passenger) across the OM of Gram-negative bacteria. Despite some controversial data, it has been widely accepted that N-passenger domains of AT must be unfolded and devoid of disulphide bonds for efficient translocation. To address whether or not AT are able to translocate folded protein domains across the OM, we employed several types of recombinant antibodies as heterologous N-passengers of the transporter C-domain of IgA protease (C-IgAP) of Neisseria gonorroheae. The N-domains used were single chain Fv fragments (scFv) and variable mono-domains derived from camel antibodies (VHH) selected on the basis of their distinct and defined folding properties (i.e. enhanced solubility, stability and presence or not of disulphide bonds). Expression of these hybrids in Escherichia coli shows that stable scFv and VHH domains are efficiently (〉99%) translocated towards the bacterial surface regardless of the presence or not of disulphide bonds on their structure. Antigen-binding assays demonstrate that surface-exposed scFv and VHH domains are correctly folded and thus able to bind their cognate antigens. Expression of scFv- or VHH-C-IgAP hybrids in E. coli dsbA or fkpA mutant cells reveals that these periplasmic protein chaperones fold these N-domains before their translocation across the OM. Furthermore, large N-passengers composed of strings of VHH domains were secreted in a folded state by AT with no loss of efficacy (〉99%) despite having multiple disulphide bonds. Thus AT can efficiently translocate toward the cell surface folded N-passengers composed of one, two or three immunoglobulin (Ig) domains, each with a folded diameter between ∼2 nm and having disulphide bonds. This tolerance for folded protein domains of ∼2 nm fits with the diameter of the central hydrophilic channel proposed for the ring-like oligomeric complex assembled by C-IgAP in the OM.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-2958.2004.04014.x
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