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  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Plant cell reports 10 (1991), S. 152-155 
    ISSN: 1432-203X
    Keywords: Daucus ; Regeneration ; Ethylene ; Protein ; Isozymes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Cultures derived from domestic carrot (Daucus carota L.) inbreds were found to vary with respect to regeneration potential as measured by the production of somatic embryos in suspension cultures. A number of biochemical parameters previously reported to distinguish embryogenic from non-embryogenic cultures of other species were measured in these carrot cell lines. Ethylene production was found to be inversely related to regeneration potential. The cell line producing the greatest number of somatic embryos exhibited the lowest rate of ethylene biosynthesis, even when grown on 2, 4-D-containing maintenance medium. A specific isozyme of acid phosphatase was associated with embryogenic calli. Proteins visualized by SDS-PAGE did not discriminate between embryo-forming and proliferating calli in all inbreds.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Plant cell reports 8 (1989), S. 207-209 
    ISSN: 1432-203X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Triglycerides in developing zygotic embryos of Norway spruce and loblolly pine were found to accumulate continuously during the course of development, comprising nearly 50% of the fresh weight of a mature embryo. Embryogenic calli of these two species contained dramatically lower levels of triglycerides. Abscisic acid treatments promoted both embryo production and triglyceride accumulation in Norway spruce cultures. A method used to determine triglyceride levels in human serum, commercially available in kit form, was adapted for use with plant tissues. Low levels of triglycerides in the cultured tissues may be related to difficulties in the development and germination of conifer somatic embryos.
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Plant growth regulation 3 (1985), S. 319-327 
    ISSN: 1573-5087
    Keywords: Aspen ; DCHA ; embryogenesis ; MGBG ; polyamines ; spermidine ; wild carrot
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The spermidine synthesis inhibitors methylglyoxal bis-(guanylhydrazone) (MGBG) and dicyclohexylammonium sulfate (DCHA) were found to reduce growth and embryogenesis in wild carrot cultures. Cellular polyamine levels were also affected by the inhibitors, with spermidine levels being especially reduced by DCHA. Similarly, MGBG reduced organogenetic development of shoots on excised aspen hypocotyls. These data suggest that the polyamines, especially spermidine, play an important role in the growth and development of plants.
    Type of Medium: Electronic Resource
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