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  • 1
    Electronic Resource
    Electronic Resource
    The effect of ferric ion on the rate of ferrous oxidation by Thiobacillus ferrooxidans (1996)
    Springer
    Applied microbiology and biotechnology 45 (1996), S. 688-691 
    Details
    ISSN: 1432-0614
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract  In this study, the effect of ferric ion and cell concentrations on the oxidation of ferrous ion by T. ferrooxidans was investigated. Ferric ions competitively inhibited ferrous ion oxidation by the bacteria. The inhibitory effect of ferric ion was, however, reduced by increasing cell concentration. The apparent ferric ion inhibition constant did not change with increasing cell concentration. The ferrous ion oxidation kinetics in the absence and presence of ferric ion changes from the standard Michaelis-Menten type at low cell concentrations to pseudo-first-order kinetics at high cell concentration.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002530050749
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  • 2
    Electronic Resource
    Electronic Resource
    Monitoring the biological treatment of anthracene-contaminated soil in a rotating-drum bioreactor (1995)
    Springer
    Applied microbiology and biotechnology 43 (1995), S. 521-528 
    Details
    ISSN: 1432-0614
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract A 2-kg-capacity rotating-drum reactor was used for biological conversion of nearly insoluble organic contaminants in soil. The rotating motion allowed effective operation at a solids content of over 60% by weight. A mixed bacterial culture was used to degrade anthracene that had been impregnated into a representative high-clay soil. The activity of the culture was sustained over a period of months in repeated batch operation, in which fresh soil was inoculated with 20% spent slurry from the previous run. Maximum degradation rates of 100–150 mg anthracene (kg soil)−1 day−1 were achieved throughout the experiments. Evolution of carbon dioxide from the bioreactor showed that degradation and mineralization of anthracene occurred simultaneously, and that 55% of the anthracene was mineralized. When the culture was switched from anthracene as sole carbon source to a mixture of three polynuclear aromatic hydrocarbons, the culture was able to degrade each of these in the sequence: anthracene, phenanthrene and finally pyrene.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00218460
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  • 3
    Electronic Resource
    Electronic Resource
    Kinetics of chemically modified lignin peroxidase and enzymatic oxidation of aromatic nitrogen-containing compounds (1995)
    Springer
    Applied microbiology and biotechnology 42 (1995), S. 675-681 
    Details
    ISSN: 1432-0614
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract Lignin peroxidase from the white-rot fungus Phanerochaete chrysosporium was chemically modified by reductive alkylation with benzyl, naphthyl and anthracyl moieties, thereby increasing its superficial hydrophobicity. The three chemical modifications altered the kinetic behaviour of the enzyme in 10% acetonitrile with four different substrates: carbazole, pinacyanol, pyrene and veratryl alcohol. Benzyl modification of lignin peroxidase increased the catalytic efficiency (k cat/K m,app) 2.7 times for carbazole oxidation. Thirteen N-containing compounds, including pyrroles, pyridines, and aromatic amines, were tested to determine whether they could be oxidized by lignin peroxidase in 10% acetonitrile. All the pyrrole analogues and all the amines tested were oxidized, but none of the pyridine analogous reacted. Some products were isolated and analyzed by high-resolution mass spectrometry. Most were dimers or polymers and, in some cases, these contained oxygen atoms. The possibility of bitumen and petroleum modifications using this enzyme is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00171943
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  • 4
    Electronic Resource
    Electronic Resource
    Kinetics of chemically modified lignin peroxidase and enzymatic oxidation of aromatic nitrogen-containing compounds (1995)
    Springer
    Applied microbiology and biotechnology 42 (1995), S. 675-681 
    Details
    ISSN: 1432-0614
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract  Lignin peroxidase from the white-rot fungus Phanerochaete chrysosporium was chemically modified by reductive alkylation with benzyl, naphthyl and anthracyl moieties, thereby increasing its superficial hydrophobicity. The three chemical modifications altered the kinetic behaviour of the enzyme in 10% acetonitrile with four different substrates: carbazole, pinacyanol, pyrene and veratryl alcohol. Benzyl modification of lignin peroxidase increased the catalytic efficiency (k cat/K m,app) 2.7 times for carbazole oxidation. Thirteen N-containing compounds, including pyrroles, pyridines, and aromatic amines, were tested to determine whether they could be oxidized by lignin peroxidase in 10% acetonitrile. All the pyrrole analogues and all the amines tested were oxidized, but none of the pyridine analogous reacted. Some products were isolated and analyzed by high-resolution mass spectrometry. Most were dimers or polymers and, in some cases, these contained oxygen atoms. The possibility of bitumen and petroleum modifications using this enzyme is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002530050312
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  • 5
    Electronic Resource
    Electronic Resource
    Monitoring the biological treatment of anthracene-contaminated soil in a rotating-drum bioreactor (1995)
    Springer
    Applied microbiology and biotechnology 43 (1995), S. 521-528 
    Details
    ISSN: 1432-0614
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract A 2-kg-capacity rotating-drum reactor was used for biological conversion of nearly insoluble organic contaminants in soil. The rotating motion allowed effective operation at a solids content of over 60% by weight. A mixed bacterial culture was used to degrade anthracene that had been impregnated into a representative high-clay soil. The activity of the culture was sustained over a period of months in repeated batch operation, in which fresh soil was inoculated with 20% spent slurry from the previous run. Maximum degradation rates of 100–150 mg anthracene (kg soil)-1 day-1 were achieved throughout the experiments. Evolution of carbon dioxide from the bioreactor showed that degradation and mineralization of anthracene occurred simultaneously, and that 55% of the anthracene was mineralized. When the culture was switched from anthracene as sole carbon source to a mixture of three polynuclear aromatic hydrocarbons, the culture was able to degrade each of these in the sequence: anthracene, phenanthrene and finally pyrene.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002530050445
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  • 6
    Electronic Resource
    Electronic Resource
    Degradation of sulfur heterocycles in prudhoe bay crude oil by soil enrichments (1984)
    Springer
    Water, air & soil pollution 21 (1984), S. 225-230 
    Details
    ISSN: 1573-2932
    Source: Springer Online Journal Archives 1860-2000
    Topics: Energy, Environment Protection, Nuclear Power Engineering
    Notes: Abstract Soil was incubated in mineral medium with Prudhoe Bay crude oil at 20 °C under shake-flask conditions. The aromatic fractions of oil extracted from these cultures were analyzed by capillary GC using a S-specific detector. Without nutrient supplementation, these cultures degraded alkyl benzothiophenes, dibenzothiophene, the Cl-dibenzothiophenes and some C2 dibenzothiophenes after 27 days incubation. when supplemented with N and P, most of the C2 dibenzothiophenes and some C3-dibenzothiophenes were also degraded. The following orders of susceptibility to microbial attack were observed in homologous series: (a) C2 benzothiophene 〉 C3-benzothiophenes,(b)dibenzothiophene 〉 C1-dibenzothiophenes 〉 C2 dibenzothiophenes 〉 C3-dibenzothiophenes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00163626
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  • 7
    Electronic Resource
    Electronic Resource
    Degradation of aromatics and saturates in crude oil by soil enrichments (1981)
    Springer
    Water, air & soil pollution 16 (1981), S. 367-375 
    Details
    ISSN: 1573-2932
    Source: Springer Online Journal Archives 1860-2000
    Topics: Energy, Environment Protection, Nuclear Power Engineering
    Notes: Abstract Soil was incubated in the presence of Prudhoe Bay crude oil at 20°C under shake-flask conditions. With the addition of N and P to the medium, the susceptibility of aromatic compounds to microbial degradation over an 11-day period was found to be: naphthalene ≈ 2-methylnaphthalene 〉 1-methylnaphthalene 〉 dimethylnaphthalenes ≈ dibenzothiophene ≈ phenanthrene 〉 C3-naphthalenes 〉 methylphenanthrenes 〉 C2-phenanthrenes. Then-alkanes and isoprenoids (pristane and phytane) were also extensively degraded during this time. The omission of N and P from the medium reduced the extent to which the culture degraded then-alkanes but had little effect on the degradation of the aromatic fraction after a 27-day incubation period.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01046917
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  • 8
    Electronic Resource
    Electronic Resource
    Kinetics of methanogenic degradation of phenol by activated-carbon-supported and granular biomass (1992)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 40 (1992), S. 777-786 
    Details
    ISSN: 0006-3592
    Keywords: phenol degradation ; methanogensesis ; activated carbon ; Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: A continuous-feed recycle bioreactor was used to study the kinetics of methanogenic degradation of phenol at 35°C by bacteria supported on a bed of granular activated carbon (GAC). At dilution rates well above the growth rate of the culture, the cells not only populated the GAC, but also formed a layer of granular biomass. This layer was stabilized by the presence of the GAC, and accounted for over half of the phenol-degrading activity in the bioreactor. The specific phenol degradation rates for GAC-attached biomass, suspended biomass, and granular biomass were all in the range 0.15 to 0.22 mg phenol/mg volatile solids per day as measured under pseudo-steady-state conditions. © 1992 John Wiley & Sons, Inc.
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bit.260400705
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  • 9
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    Kinetics of chemically modified lignin peroxidase and enzymatic oxidation of aromatic nitrogen-containing compounds (1995)
    Springer
    Details
    Publication Date: 1995-01-01
    Print ISSN: 0175-7598
    Electronic ISSN: 1432-0614
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Published by Springer
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  • 10
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    Monitoring the biological treatment of anthracene-contaminated soil in a rotating-drum bioreactor (1995)
    Springer
    Details
    Publication Date: 1995-07-01
    Print ISSN: 0175-7598
    Electronic ISSN: 1432-0614
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Published by Springer
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