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  • 1
    ISSN: 1432-1211
    Keywords: Key words Aotus nancymaae ; Immunoglobulin kappa light chain (IGK) genes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract  Sequences of Aotus nancymaae immunoglobulin kappa light-chain rearrangements were analyzed after reverse transcription polymerase chain reaction. Among 22 in-frame rearrangements analyzed, 12 IGKV genes belonging to the families 1, 2, or 3 were identified. Aotus counterparts for all five human IGKJ genes were found. The identity of the deduced human and Aotus amino acid sequences was between 83% and 92% for junctional regions and 74% for the constant region. Sequence comparisons between rearrangements indicated that somatic mutations, the addition of nongermline-encoded nucleotides, and exonuclease trimming contribute to the generation of diversity of Aotus immunoglobulin kappa chains. The high identity of Aotus and human IGK genes is comparable to that of T-cell receptor genes and further supports the proposal to use the Aotus Plasmodium falciparum infection model for the evaluation of malaria vaccine candidates.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Journal of the American Water Resources Association 32 (1996), S. 0 
    ISSN: 1752-1688
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Architecture, Civil Engineering, Surveying , Geography
    Notes: : The ability to predict how streams and wetlands retain phosphorus (P) is critical to the management of watersheds that contribute nutrients to adjacent aquatic systems such as lakes. Field and laboratory experiments were conducted to determine the P assimilatory capacity of a stream (Otter Creek) in the Taylor Creek/Nubbin Slough Basin located north of Lake Okeechobee, Florida. Dominant soils in this basin are sandy Spodosols; landuse is primarily dairy farms and beef cattle pastures. Estimates of P assimilation show that sediments assimilate approximately 5 percent of the P load. Phosphorus assimilation rates in the stream were estimated using first-order relationships based on the total P concentration of the water column as a function of distance from the primary source. This method assumes minimal lateral inputs. Stream lengths required for one turnover in P assimilation were estimated to be in the range of 3–16 km. Laboratory studies using intact sediment cores indicated a P assimilation rate of 0.025 m day−1, and equilibrium P concentration of 0.16 ± 0.03 mg L−1 in the water column. Dissolved P concentration gradients in the sediments showed upward flux of P at water column P concentration of 〈0.16 mg L−1. Approximately 56–77 percent of the P assimilated in the above-ground vegetation during active growth was released or translocated within six months of senesence, suggesting short-term storage in above-ground vegetation. Bottom sediments and recalcitrant detrital plant tissue provide for long-term P assimilation in the creek. Although stream sediments have the potential to adsorb P, high flow rate and low contact period between water and sediment limits this process.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Amsterdam : Elsevier
    Meat Science 34 (1993), S. 205-216 
    ISSN: 0309-1740
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Aquaculture research 29 (1998), S. 0 
    ISSN: 1365-2109
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: An allozyme survey was conducted in three naturalized populations of rainbow trout, Oncorhynchus mykiss (Walbaum), from rivers in two Andean lakes of southern Chile. The analysis was based on 32 presumed loci, 10 of which were variable, and revealed that the mean percentage of polymorphic loci (P= 21.8%; range = 15.6-28.1%), the mean number of alleles per locus (n=1.26; range = 1.2-1.3) and the mean observed heterozygosity (HO=7.0%; range = 6.0-8.2%) are high, although within the range reported for rainbow trout. Although the mean genetic distance between the samples was low (D = 0.003), and only 5.2% of the total observed genetic variation can be accounted by interpopulation differences (FST= 0.052), eight out of 10 polymorphic loci exhibited significant allelic heterogeneity. Some loci, notably EST-1*, IDHP-3,4*, MEP-1* and PGM-1*, showed considerable discriminatory capacity among the samples. Rainbow trout has a longer history in Chilean waters since salmonid introduction started earlier this century. Under current intensive farming practices, the preliminary information provided in the present paper is likely to be helpful in setting up a baseline for further characterization and/or monitoring of naturalized populations.
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  • 5
    ISSN: 0308-8146
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Type of Medium: Electronic Resource
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  • 6
    Electronic Resource
    Electronic Resource
    Springer
    Theoretical and applied genetics 88 (1994), S. 717-721 
    ISSN: 1432-2242
    Keywords: Maize ; Linkage map ; Sequence tagged sites ; Expressed sequence tags
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract We report here the results of mapping a set of 92 leaf cDNA clones in maize. The ends of each of these cDNA clones have previously been partially sequenced, and the sequence comparison has revealed the putative function for 28 clones. It is expected that the RFLP map developed using these expressed sequence tags will be of great importance for future maize genome analysis, such as for PCR-based gene mapping or gene function identification.
    Type of Medium: Electronic Resource
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  • 7
    Electronic Resource
    Electronic Resource
    Springer
    Theoretical and applied genetics 99 (1999), S. 210-217 
    ISSN: 1432-2242
    Keywords: Key words Triticeae ; Elymus alaskanus ; Allozymes ; Genetic differentiation ; Diversity
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  To gain information on the extent and nature of genetic variation in Elymus alaskanus, levels and distribution of genetic variation were assessed within and among 13 populations originating from Iceland, Norway, Sweden and Russia using allozymes. The results showed that four (30.7%) of the 13 loci were polymorphic within the species, while the mean percentage of polymorphic loci within the populations was 1.9%. The mean number of alleles per locus for the species was 1.8 and 1.02 across the populations. Genetic diversity at the species level was low (H es=0.135), and mean population diversity was notably lower (H ep=0.005). A high degree of genetic differentiation was observed among populations. The salient points emerging from this study are: (1) statistically significant differences were found in allele frequencies among populations for every polymorphic locus (P〈0.001), (2) the high mean coefficient of gene differentiation (G ST) showed that 95% of the total allozyme variation was attributable to differences among populations, and (3) relatively high genetic distances between the populations were obtained (mean D=0.16). The Norwegian populations had the highest genetic diversity as compared with the other populations. Geographical comparisons revealed three different groups of populations clearly differentiated, i.e. Scandinavia (Norway and Sweden), Iceland and Russia. Cluster and principal coordinates analyses revealed the same genetic patterns of relationships among populations. Generally, this study indicates that E. alaskanus contains low allozymic variation in its populations. The implications of these results for the conservation of the species are discussed.
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  • 8
    ISSN: 1572-8927
    Keywords: Activity coefficients ; NaBr+Na-Formate ; Scatchard's, Pitzer's and Lim's methods
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract Activity coefficients for NaBr in the system NaBr+NaFormate+H2O at 25°C were determined from emf measurements at different total ionic strengths. At each total ionic strength, the measurements were carried out at different Na-Formate/NaBr ionic strength ratios. The experimental activity coefficients were comparatively analyzed using Scatchard's, Pitzer's and Lim's methods. Although all these models can be successfully applied to the analysis of this mixed system, the All Mixing Coefficients (LA) and Consistency Test (LT) models lead to better fittings than the others.
    Type of Medium: Electronic Resource
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  • 9
    Electronic Resource
    Electronic Resource
    Springer
    Journal of solution chemistry 24 (1995), S. 551-563 
    ISSN: 1572-8927
    Keywords: Activity coefficients ; ethanol-water mixtures ; NaBr ; emf ; association
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract Activity coefficients for NaBr in ethanol-water mixtures with 0, 20, 40, 60, 70, 80, 90 and 99.9 weight% of ethanol have been determined at 25°C from the emf measurements of the galvanic cells $$Na - glass/NaBr(m),H_2 O(100 - Y),EtOH(Y),AgBr_{(S)} /Ag$$ The results obtained were analyzed by using the Pitzer equation. Those for the mixtures with ethanol content higher than 60 wt% were also analyzed by using a chemical model and their ion pair formation constants, K A 0 , determined and examined. The results were compared with those for NaCl reported in a previous paper.
    Type of Medium: Electronic Resource
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  • 10
    ISSN: 1573-5060
    Keywords: Apion godmani ; bean pod weevil ; common bean ; Phaseolus vulgaris ; insect resistance ; breeding
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The larva of the bean pod weevil (BPW), Apion godmani Wagner (Coleoptera: Curculionidae), causes serious yield losses in common bean (Phaseolus vulgaris L.) in Mexico and Central America, by consuming the seed as it develops in the immature pod. Resistance to the BPW was identified in bean germplasm of highland Mexican origin, and these sources of resistance were incorporated into a pedigree breeding program to recover locally adapted lines resistant to Bean Common Mosaic Virus and BPW, with commercial grain for Guatemala, Honduras and El Salvador. These lines yielded as well as or better than local cultivars in the absence of the insect, and better than local cultivars when the BPW was present. Resistance appeared to be governed by several genes, and was stable across geographic areas, seasons and planting systems.
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