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  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Molecular genetics and genomics 107 (1970), S. 39-49 
    ISSN: 1617-4623
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The alteration of the component upon mutation from spectinomycin sensitive (spc s) to resistant (spc r) was found only on a specific 30s ribosomal protein component, 30-4. in E. coli strain K 12 (Dekio and Takata, 1969), or on its alleic component, 30-4 (B) in strain B or 30-4 (R) in strain IAM 1182. Similarly, mutation from erythromycin-sensitive (ery s) to resistant (ery r) accompanied the alteration of a specific 50s ribosomal protein component, 50-8, in E. coli strain Q 13 (Otaka Teraoka, Tamaki, Tanaka and Osawa, 1969), or its counterpart, 50-8 (R), in strain IAM 1132.
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Molecular genetics and genomics 109 (1970), S. 131-141 
    ISSN: 1617-4623
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Salmonella typhimurium L+15-11 was shown to have four 30s and six 50s ribosomal protein components which were chromatographically distinguishable from their counterparts of Escherichia coli K-12 JC411E01. A hybrid strain between E. coli K-12 JC411E01 and S. typhimurium L+15-11, having ribosomes of S. typhimurium-type protein composition and being sensitive to phage Plkc was isolated by conjugation between these two species. Transduction experiments by phage Plkc between the above hybrid strain and E. coli K-12 JC411ES01 showed that characters of all the S. typhimurium-type ribosomal protein components could be cotransduced with the streptomycin or spectinomycin resistance marker, indicating that the genes specifying these ten ribosomal protein components were located in the str region on the chromosome.
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Molecular genetics and genomics 107 (1970), S. 32-38 
    ISSN: 1617-4623
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The comparative chromatographic investigations into the ribosomal proteins of various strains of E. coli have demonstrated that most of the strains including three strains of E. coli subsp. communior had ribosomes with the same protein compositions (C-type). The ribosomes from strain B are different from the C-type ribosomes in having the specific 30-4 (B) component in place of 30-4 (B-type), while those from strains K 12 may be distinguished from the type-C ribosomes by the presence of the specific 30-7 (K) component in place of 30-7 (K-type) or, in addition to 30-7 (K), the presence of 30-9 (W3637) in place of 30-9 (K-3637 type). Two strains, IAM 1132 and IAM 1182, have R-type ribosomes, in which at least six 50s proteins and four 30s protein components are distinct from the corresponding protein components in the C-type ribosomes.
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  • 4
    Electronic Resource
    Electronic Resource
    Springer
    Molecular genetics and genomics 105 (1969), S. 219-224 
    ISSN: 1617-4623
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Spectinomycin resistant (spc r) mutants were obtained by treating the cells of E. coli K12, W3637 with nitrosoguanidine. The compositions of ribosomal proteins were analyzed for six out of eleven such spc r-mutants with chromatography on a carboxymethyl cellulose (CMC) column. The 30s ribosomal subunit from all of the spc r-mutants was found to contain the altered 30-4 protein component, while no difference was detected in 50s ribosomal proteins between spc r and spc s bacteria.
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  • 5
    Electronic Resource
    Electronic Resource
    Springer
    Molecular genetics and genomics 105 (1969), S. 113-121 
    ISSN: 1617-4623
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Two 30s ribosomal protein components, 30-4K and 30-7K, from E. coli K12 strain were clearly distinguished on the CMC column chromatogram from the corresponding protein components, 30-4B and 30-7B, from B strain. The 30-7K component was shown to correspond to the K-character. A mutant strain of K12, W3637, had an altered 30s ribosomal protein component, 30-9W3637. The characters of 30-4K, 30-7K, 30-9W3637 and str r were found to be cotransduced from W3637 to B strain by Plke phage in 16 out of 20 str r transductants. The 30-9W3637 and 30-4K components were separated from str r in 4 str r transductants. These results indicate that (1) neither 30-4 nor 30-9 is the protein whose mutational change leads to str r, and (2) the genes specifying the 30s ribosomal proteins, 30-4, 30-7, 30-9 and str are linked on the chromosome.
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