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  • 1
    Electronic Resource
    Electronic Resource
    Direct shoot regeneration from excised leaf explants of in vitro grown seedlings of Alstroemeria L. (1997)
    Springer
    Plant cell reports 16 (1997), S. 770-774 
    Details
    ISSN: 1432-203X
    Keywords: Key wordsAlstroemeria ; Regeneration ; Thidiazuron ; Silver nitrate ; Ancymidol
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A two-step protocol for the induction of shoots from Alstroemeria leaf explants has been developed. Leaf explants with stem node tissue attached were incubated on shoot induction medium for 10 days, and then transferred to regeneration medium. Shoots from the area adjacent to the region between the leaf base and node tissue regenerated within 3 weeks after transfer to the regeneration medium, without a callus phase. The best induction was obtained with Murashige and Skoog medium containing 10 µm thidiazuron and 0.5 µm indole butyric acid. The regeneration medium contained 2.2 µm 6-benzylaminopurine. After several subcultures of the leaf explants with induced shoots, normal plantlets with rhizome were formed. In Alstroemeria, the percentage of responding leaf explants is more important than the number of shoots regenerated per leaf explant, because rhizome formation is the most important factor for micropropagation. The effect of other compounds in the induction medium, including glucose, sucrose, silver nitrate, and ancymidol, on regeneration was also investigated.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002990050317
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  • 2
    Electronic Resource
    Electronic Resource
    Optimization of AFLP fingerprinting of organisms with a large-sized genome: a study on Alstroemeria spp. (1999)
    Springer
    Theoretical and applied genetics 98 (1999), S. 465-471 
    Details
    ISSN: 1432-2242
    Keywords: Key words AFLP genetic marker ; Alstroemeriaceae ; Inca lily ; Multiple cascade PCR ; Preamplification
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  The recently introduced PCR-based DNA fingerprinting technique AFLP (amplified fragment length polymorphism) allows the selective amplification of subsets of genomic restriction fragments. AFLP has been used for multiple purposes such as the construction of linkage maps, marker saturation at specific genomic regions, analysis of genetic diversity and molecular phylogeny and cultivar identification. AFLP can be tailored by varying the number of selective nucleotides added to core primers and can allow accurate amplification, even in complex template mixtures generated from plant species with very large genomes. In this study Alstroemeria, a plant species with a very large genome, was tested for adapting the AFLP protocol. The results indicated that the estimated number of amplification products was close to the observed number when eight selective nucleotides were used but that seven selective nucleotides did not increase the number of amplification products fourfold. However, we found reproducibility in both +7 and +8 fingerprints. Various distributions of selective nucleotides over the various rounds of preamplifications were tested. Preamplification with four selective nucleotides followed by final amplification with eight selective nucleotides produced clear and reproducible AFLP patterns. The effects of GC content of primers and multiple preamplification steps were also discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001220051093
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