Solid-phase peptide synthesis
Disperse Red 1
Wiley InterScience Backfile Collection 1832-2000
Chemistry and Pharmacology
A new simple and efficient method for the detection of incomplete coupling reactions during solid-phase peptide synthesis is decribed. Using p-nitrophenyl ester 1 (NF31), free amino groups can be visually detected on the resin by direct coloring of the beads. A specific feature of the assay resides in the possibility of detection of sterically hindered primary amines.
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