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  • 1
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    Preparation of inactivated vaccine to induce resistance against white spot syndrome virus by nuclear & non nuclear techniques (2018)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    Details
    Publication Date: 2021-05-19
    Description: The inactivated of white spot syndrome virus (WSSV) by Gama and Electron radiation and chemical material such as Formalin with antigen property have been done during last year. At the first time the stock of virus prepared and purred it. Six hundred of shrimp Fenneropenaus indicus with average weight 7 to 12 gram collected from shrimp research station in Heleh area and transported to Shrimp Research Institute in Boushehr province. Vibrio parahemolyticus obtained from Veterinary University of Tehran and then lyophilized it. The bacterial V. parahemolyticus inactivated with Gamma, electron and formalin and then injected to shrimp for activated the immune system. The source of viruses was identified through PCR, TEM and histopathology methods from the shrimp infected in 2009 occurrence of boushehr province. The virus injected to crayfish and collected the hemlymph for prepared the stock of WSSV virus. The virus was lyophilized and then exposed to gamma and electron radiation and formalin with optimal dose for inactivated the virus. The shrimp divided to 25 groups and each group consist of six shrimp and the vaccine exposed to shrimp with injected and bathroom methods and the documented the result after 15 days. In vivo virus titration was performed in Penaeus indicus. Inactivation of WSSV was carried out by a gamma cell instrument Nordian, model 220 with dose rate: 4.8 Gy/sec and activity: 20469 Ci. The LD50 of live virus stock was calculated 10 5.4 / ml and the optimum dose of gamma radiation beam to inactivate WSSV was obtained 14-15 kGy. The LD50 of live virus stock was calculated 10 5.4 / ml and the optimum dose of electron beam to inactivate WSSV was obtained 12-13 kGy and and the virus inactivated by formalin 0.5% V/V during 10 minutes.The LD50 of live virus stock was calculated by Karber method 10 3.29 /ml and 10 5.35 /ml, respectively. The V. parahemolyticus was inactivated with 8 KG. The result showed all vaccine during 2 hours have a good effect to shrimp viruses with the102.4 LD50 / 50 l titre. The result showed if the Gamma and electron vaccine increase to feed of shrimp the effect of vaccine is better than when used without feed. The end of experiment our study showed that the Gamma vaccine has the better effect to control WSSV during 2 hours with comparing the others.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Vaccine ; White Spot Syndrome Virus ; Gamma irradiation ; Electron Radiation ; Formalin ; Penaeus indicus ; WSSV ; Fenneropenaus indicus ; Shrimp ; V. parahemolyticus ; Preparation
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 148pp.
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  • 2
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    Incidence of white spot disease (WSD) in Penaeus indicus farms in Bushehr Province, Iran (2018)
    Details
    Publication Date: 2021-05-19
    Description: The incidence of white spot disease in farmed Penaeus indicus in Busheher region was evaluated by analyzing 200 shrimp individuals between June and September, 2005. The samples were examined for clinical sign including observation of white spots on the cuticle and tissue. Samples were processed using polymerase chain reaction (Nested-PCR) test. The results showed that 24% of the samples examined were clinically positive having white spots on their cuticles. Also, 92% of the samples examined were positive in PCR examination. Furthermore, 70% of apparently healthy shrimp were positively identified using the by PCR test.
    Description: Published
    Keywords: Incidence ; White spot disease ; Penaeus indicus ; Nested PCR ; Clinical sign
    Repository Name: AquaDocs
    Type: Journal Contribution , Refereed
    Format: pp.15-26
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  • 3
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    Assessing pathogenesis of the white spot syndrome virus (WSSV) in the white-leged shrimp (Litopenaeus vannamei) (2018)
    Details
    Publication Date: 2021-05-19
    Description: The pathogenesis of the white spot syndrome virus (WSSV) was studied experimentally with challenge exposure of one hundred post larvae Litopenaeus vannamei each aged 40 days. The shrimp L. vannamei larvae were first examined with Shrimp PC sub(a)R sub(e) Simplex detection kit and found to be free of WSSV. The Fenerropenaeus indicus tissues that showed the clinical signs and were PCR positive for the white spot disease (WSD) were used as the source of WSSV. The challenge exposure was accomplished by feeding minced tissue of the F. indicus to L. vannamei for 24 hours. Based on the results, the L. vannamei revealed the clinical sign of WSD after three days, the PCR was positive and all the shrimp specimens were died after ten days. The control group of the shrimp did not show any sign of WSD and their PCR was negative during the experiment.
    Description: Published
    Keywords: Whiteleg shrimp ; Pathogenesis
    Repository Name: AquaDocs
    Type: Journal Contribution , Refereed
    Format: pp.1-8
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  • 4
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    Implementation biosecurity standard in shrimp specific pathogen free (SPF) research pilot study (2018)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    Details
    Publication Date: 2021-05-19
    Description: Biosecurity means” all activities that decrease the introducing and expanding the pathogen in the shrimp farm” the main activity consisit physical, chemical and biological methods. Some factores has low risck and some of them have high risck level. The level of biosecurity in the shrimp farm and hatcheries is depends the equipmemt and expense that used in the program. For each situation in biosecurity, the scientist will be prepared the standard operation program (SOP) for each risck factor.In the Specific Pathogen Free program that conduted in Bousher, 10 SOP design for different risck factor in the banderga station and Persian Golf Centre for SPF production. The SOP, detecting the main pathogen in shrimp farm in shrimp, feed, water and any equipment in the project. The SOP also consider the disinfectant water with physical and chemival methods such as different net in different size, chemical such as chlorine and ozon and biological filter. During the project studied the methods exclude all pathogen from shrimp and feed and decreased the risck factors in program. The result showed if the culturist used these methods in the production, the safty of shrimp production increased and the sustainable shrimp culture will be available.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Biosecurity ; Ciritical point ; Standard Operation Porogram ; Disaese ; SOP ; Implementation
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 65pp.
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  • 5
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    The Study on health situation of shrimp Litopenaus vannamei broodstoock production in earthen pond and comparing with fiberglass or cement tanks (2018)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    Details
    Publication Date: 2021-05-19
    Description: The investigation of health and diseas situation of shrimp broodstook (Litopenaeus vannamei) in Boushehr province in earthen pond and cancreat and fiberglas was carried out from May 2014 until July 2014 with collected 100 samples brood stook from earthen pond and 100 samples from earth and fiberglas thank.The clinical sign of samples documented in take history form and then the samples transport to Iran Shrimp Research Instituet in Bousheher. The bacterial and fungal studied was carried out with hemolymp, hepatopacreas and gill tissue and then the shrimp preserved in Davidson Fixative for histopathology. A part of uropoda also preserved in ethyl alcholo for PCR study and detecting viruses. The result showed 10 bacteria consist V.harveyii, V.parahaemolyticus, V.anguillarum, V.vulnificus, V.mimicus, V.damsela,V.nereis, plesiomonas shigelloides V.alginolyticus V.proteolyticus, in earthen pond and 5 bacteria consit ,V.alginolyticus V.proteolyticus ، V.parahaemolyticus ، V. damsela ،V.mimicus were identified in fiberglas thank. In this study 10 fungi consist Penecilium, Asp. Niger, Asp. Flavious, Asp. fomigatus, Acromonium, Ulocladium, Mucor, Cladosporium, Alternaria, Rhizopus, and 5 of them were identified in both broodstock from earthen pond and fiberglas thank. However 6 parasite consist of zoothamnium, vorticella, Acneta, Ephelota, Epistylis, Epistylis and Apastomom identified in earthen pond and three of them were identified in fiberglas thank. In histoplatholgy some tissue showed the effect of vibrio infecting in different organs as well in gill and midgut and the PCR examined were negative for all viruses. Regarding the produce healthy broodstock we need excuted the High Health procdure.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Shrimp broodstock ; Health ; Disaes ; Histopathology ; PCR ; Litopenaeus vannamei ; V.harveyii ; V.parahaemolyticus ; V.anguillarum ; V.vulnificus ; V.mimicus ; V.damsela ; V.nereis ; V.alginolyticus ; V.proteolyticus ; Earthen Pond ; Fiberglass ; Tank
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 87pp.
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  • 6
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    (Fenneropenaeus indicus) study on protozoa emerging diseases of shrimp (2018)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    Details
    Publication Date: 2021-05-19
    Description: Certain diseases such as bacterial , viruses, parasirtic ones Protozoa parasites Could severe mortalities in farmed shrimp last decades. Two hundred and seventy Fenneropenaeus indicus with the weight of 10± 1.09 were selected , transferred to Iran Shrimp Center and distributed to the treatmentsand controls (3 groups). The objective of our study was to describe the signs, prevalence, and intensity of Hemocytosis, a new disease in shrimp, Fenneropenaeus indicus. The experimental design included three groups, one treatment as infected shrimp or group B and 2 controls with non-infected but exposure to White Spot Syndrom Virus (WSSV) shrimp as group A and non-infected non- exposure to WSSV shrimp as group C in triplicate. The results revealed that Hemocytosis associated with a decrease in hyalinocytes and large-granulocytes (less than 8%) and a considerable increase in semi-granulocytes in group A and B in contrast to group C that Hyalinocyte increased. This infection made the shrimp susceptible to WSSV disease. It is believed that Hemocytosis causes a condition which can result in a rapid mortality among susceptible species, F. indicus when exposure to WSSV is occurred.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: White Spot Syndrome Virus ; Fenneropenaeus vannemai ; Challenge ; Protozoa ; Shrimp ; Fenneropenaeus indicus ; Bacterial ; Parasites ; Mortalities ; Hemocytosis ; Hyalinocyte ; Species ; F.indicus
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 56pp.
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  • 7
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    Assessing pathogenesis of the white spot syndrome virus (WSSV) in the whiteleged shrimp (Litopenaeus vannamei) (2021)
    In:  http://aquaticcommons.org/id/eprint/23622 | 18721 | 2018-07-12 19:36:06 | 23622 | Iranian Fisheries Science Research Institute
    Details
    Publication Date: 2021-07-14
    Description: The pathogenesis of the white spot syndrome virus (WSSV) was studied experimentally with challenge exposure of one hundred post larvae Litopenaeus vannamei each aged 40 days. The shrimp L. vannamei larvae were first examined with Shrimp PC sub(a)R sub(e) Simplex detection kit and found to be free of WSSV. The Fenerropenaeus indicus tissues that showed the clinical signs and were PCR positive for the white spot disease (WSD) were used as the source of WSSV. The challenge exposure was accomplished by feeding minced tissue of the F. indicus to L. vannamei for 24 hours. Based on the results, the L. vannamei revealed the clinical sign of WSD after three days, the PCR was positive and all the shrimp specimens were died after ten days. The control group of the shrimp did not show any sign of WSD and their PCR was negative during the experiment.
    Keywords: Fisheries ; Health ; Pathogenesis ; Whiteleg shrimp ; Iran
    Repository Name: AquaDocs
    Type: article , TRUE
    Format: application/pdf
    Format: application/pdf
    Format: 1-8
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  • 8
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    (Fenneropenaeus indicus) study on protozoa emerging diseases of shrimp (2021)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25494 | 18721 | 2018-10-05 07:36:19 | 25494 | Iranian Fisheries Science Research Institute
    Details
    Publication Date: 2021-07-16
    Description: Certain diseases such as bacterial , viruses, parasirtic ones Protozoa parasites Could severe mortalities in farmed shrimp last decades. Two hundred and seventy Fenneropenaeus indicus with the weight of 10± 1.09 were selected, transferred to Iran Shrimp Center and distributed to the treatmentsand controls (3 groups). The objective of our study was to describe the signs, prevalence, and intensity of Hemocytosis, a new disease in shrimp, Fenneropenaeus indicus. The experimental design included three groups, one treatment as infected shrimp or group B and 2 controls with non-infected but exposure to White Spot Syndrom Virus (WSSV) shrimp as group A and non-infected non- exposure to WSSV shrimp as group C in triplicate. The results revealed that Hemocytosis associated with a decrease in hyalinocytes and large-granulocytes (less than 8%) and a considerable increase in semi-granulocytes in group A and B in contrast to group C that Hyalinocyte increased. This infection made the shrimp susceptible to WSSV disease. It is believed that Hemocytosis causes a condition which can result in a rapid mortality among susceptible species, F. indicus when exposure to WSSV is occurred.
    Keywords: Health ; Iran ; White Spot Syndrome Virus ; Fenneropenaeus vannemai ; Challenge ; Protozoa ; Shrimp ; Fenneropenaeus indicus ; Bacterial ; Parasites ; Mortalities ; Hemocytosis ; Hyalinocyte ; Species ; F.indicus
    Repository Name: AquaDocs
    Type: monograph
    Format: application/pdf
    Format: application/pdf
    Format: 56
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  • 9
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    Implementation biosecurity standard in shrimp specific pathogen free (SPF) research pilot study (2021)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25552 | 18721 | 2018-10-05 17:30:01 | 25552 | Iranian Fisheries Science Research Institute
    Details
    Publication Date: 2021-07-16
    Description: Biosecurity means” all activities that decrease the introducing and expanding the pathogen in the shrimp farm” the main activity consisit physical, chemical and biological methods. Some factores has low risck and some of them have high risck level. The level of biosecurity in the shrimp farm and hatcheries is depends the equipmemt and expense that used in the program. For each situation in biosecurity, the scientist will be prepared the standard operation program (SOP) for each risck factor.In the Specific Pathogen Free program that conduted in Bousher, 10 SOP design for different risck factor in the banderga station and Persian Golf Centre for SPF production. The SOP, detecting the main pathogen in shrimp farm in shrimp, feed, water and any equipment in the project. The SOP also consider the disinfectant water with physical and chemival methods such as different net in different size, chemical such as chlorine and ozon and biological filter. During the project studied the methods exclude all pathogen from shrimp and feed and decreased the risck factors in program. The result showed if the culturist used these methods in the production, the safty of shrimp production increased and the sustainable shrimp culture will be available.
    Keywords: Health ; Iran ; Biosecurity ; Ciritical point ; Standard Operation Porogram ; Disaese ; SOP ; Implementation
    Repository Name: AquaDocs
    Type: monograph
    Format: application/pdf
    Format: application/pdf
    Format: 65
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  • 10
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    Preparation of inactivated vaccine to induce resistance against white spot syndrome virus by nuclear & non nuclear techniques (2021)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25345 | 18721 | 2018-09-13 12:43:08 | 25345 | Iranian Fisheries Science Research Institute
    Details
    Publication Date: 2021-07-16
    Description: The inactivated of white spot syndrome virus (WSSV) by Gama and Electron radiation and chemical material such as Formalin with antigen property have been done during last year. At the first time the stock of virus prepared and purred it. Six hundred of shrimp Fenneropenaus indicus with average weight 7 to 12 gram collected from shrimp research station in Heleh area and transported to Shrimp Research Institute in Boushehr province. Vibrio parahemolyticus obtained from Veterinary University of Tehran and then lyophilized it. The bacterial V. parahemolyticus inactivated with Gamma, electron and formalin and then injected to shrimp for activated the immune system. The source of viruses was identified through PCR, TEM and histopathology methods from the shrimp infected in 2009 occurrence of boushehr province. The virus injected to crayfish and collected the hemlymph for prepared the stock of WSSV virus. The virus was lyophilized and then exposed to gamma and electron radiation and formalin with optimal dose for inactivated the virus. The shrimp divided to 25 groups and each group consist of six shrimp and the vaccine exposed to shrimp with injected and bathroom methods and the documented the result after 15 days. In vivo virus titration was performed in Penaeus indicus. Inactivation of WSSV was carried out by a gamma cell instrument Nordian, model 220 with dose rate: 4.8 Gy/sec and activity: 20469 Ci. The LD50 of live virus stock was calculated 10 5.4 / ml and the optimum dose of gamma radiation beam to inactivate WSSV was obtained 14-15 kGy. The LD50 of live virus stock was calculated 10 5.4 / ml and the optimum dose of electron beam to inactivate WSSV was obtained 12-13 kGy and and the virus inactivated by formalin 0.5% V/V during 10 minutes.The LD50 of live virus stock was calculated by Karber method 10 3.29 /ml and 10 5.35 /ml, respectively. The V. parahemolyticus was inactivated with 8 KG. The result showed all vaccine during 2 hours have a good effect to shrimp viruses with the 102.4 LD50 / 50 l titre. The result showed if the Gamma and electron vaccine increase to feed of shrimp the effect of vaccine is better than when used without feed. The end of experiment our study showed that the Gamma vaccine has the better effect to control WSSV during 2 hours with comparing the others.
    Keywords: Health ; Iran ; Boushehr province ; Heleh area ; Vaccine ; White Spot Syndrome Virus ; Gamma irradiation ; Electron Radiation ; Formalin ; Penaeus indicus ; WSSV ; Fenneropenaus indicus ; Shrimp ; V. parahemolyticus ; Preparation
    Repository Name: AquaDocs
    Type: monograph
    Format: application/pdf
    Format: application/pdf
    Format: 148
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