ISSN:
1432-072X
Keywords:
Growth yield
;
Energetics (of growth)
;
Salmonella typhimurium
;
Glucose uptake
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract We have studied the energetics of glucose uptake in Salmonella typhimurium. Strain PP418 transprots glucose via the phosphoenolpyruvate: glucose phosphotransferase system, while strain PP1705 lacks this system and can only use the galactose permease for glucose uptake. These two strains were cultured anaerobically in glucose-limited chemostats. Both strains produced ethanol and acetate in equimolar amounts but a significant difference was observed in the molar growth yield on glucose (Y Glc). It is suggested that this difference is due to a difference in the energetics of the glucose uptake systems in the two strains. Assuming an equal Y ATP for both strains, we could calculate that uptake of 1 mole of glucose via the galactose permease consumes the equivalent of 0.5 mole of ATP. With the additional assumption that one proton is transported in symport with one glucose molecule, these results imply a stoichiometry of two protons per ATP hydrolysed.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00410936
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